Charlie Pyle
Duke University | Tobin Lab Postdoc | PharmD, PhD
Translational Scientist interested in Cell Biology — Genetics — Microbiology — Pharmacy — Innate Immunity — Zebrafish — Tuberculosis
Opinions are my own & don't represent those of my employer.
- Reposted by Charlie Pyle#Microbiology The anti-tuberculosis drug bedaquiline targets the mycobacterial ATP synthase, but how that leads to bacterial death is unclear Here the authors clarify how bedaquiline works @jnblab.bsky.social @morwan.me #TBSky #antibiotics #AMR dlvr.it/TQjZ0W
- Reposted by Charlie PyleGiven the weather today, it’s time to repost my favorite phase diagram: Nakaya’s snow crystal morphology. We are towards the bottom right today. 🧪 ⚛️ #cryoEM
- Reposted by Charlie PyleExcited to share work led by the wonderful Gopi Viswanathan examining roles of granuloma neutrophils &defining bacterial transcriptional profiles within mycobacterial granulomas. Cool collab with Qingyun Liu at UNC for evolutionary analysis of granuloma-specific genes www.science.org/doi/10.1126/...
- Reposted by Charlie PyleHuman rights activist Rigoberta Menchú was born #OTD in 1959. She has dedicated her life to publicizing the rights of #Guatemala's Indigenous peoples & to promoting Indigenous rights globally. Deservingly, she was awarded the #Nobel Peace Prize in 1992 & is a UNESCO Goodwill Ambassador.
- Reposted by Charlie PyleIn a new study from Mbano, Liu, Leslie et al., single-cell and spatial transcriptomics of human #TB lung tissues from individuals in South Africa revealed that MMP1⁺CXCL5⁺ #fibroblasts & SPP1⁺ #macrophages are linked to TB disease & TB lung #granuloma rupress.org/jem/article/... #InfectiousDisease
- Reposted by Charlie PyleConversations are a key tool for reimagining the world we want together, and curious exploration can make those conversations successful even when people don't agree
- Reposted by Charlie PylePublished version up at @natcomms.nature.com New data with Aspergillus getting into tuberculous granulomas and growing like it's a member of the extended Myco family doi.org/10.1038/s414...
- Why are prior and concurrent #TB such huge risk factors for M. abscessus infection? #zebrafish preprint lead by our research officer Denise Wee discovers tuberculous granulomas (yellow blob) act as a safe haven for opportunistic mycobacterial pathogens (magenta blobs).
- Reposted by Charlie PyleInterested in Zebrafish brain imaging? We released a 3D printed mold that's is super helpful for us. Check it out Customizable FDM-based zebrafish embryo mold for live imaging www.biorxiv.org/content/10.1... Work by Marcela and Jaakko in the lab
- Reposted by Charlie PyleA new study led by Harvard Chan School and @busph.bsky.social projects that U.S. funding cuts to global health aid will have a catastrophic effect on pediatric #tuberculosis.
- Reposted by Charlie Pyle
- Reposted by Charlie PyleFibronectin, a key component of the vessel wall, triggers smooth muscle cells to release collagen-rich extracellular vesicles that guide their movement during vessel repair and atherosclerosis. buff.ly/YOkDI4a
- Proud to present our latest work! @tobinlab.bsky.social @wallacewly.bsky.social @gregorylab.bskyverified.social Human TB granuloma transcriptional blueprints show they are dominated by SPP1 macrophages. spp1 is essential for granuloma formation in zebrafish. journals.asm.org/doi/10.1128/...
- An image I took for this project – of a granuloma Langhans MGC expressing OPN – is featured on the cover of the October 2025 issue of mBio! journals.asm.org/toc/mbio/16/10
- Reposted by Charlie PyleImportant piece from Sarah Stanley at Berkeley on new NIH definitions that can be construed to arbitrarily halt research that is both safe and important to public health and future cures www.statnews.com/2025/10/06/g... via @statnews.com
- Reposted by Charlie Pyle@kirstin-rollins.bsky.social, @mmorrissey.bsky.social et al show that #macrophages attack adherent cells using #trogocytosis instead of #phagocytosis. Reducing adhesion increased phagocytosis. Mitotic cells, which disassemble adhesions, are more vulnerable to phagocytosis rupress.org/jcb/article/...
- Reposted by Charlie PyleNew Element in the Philosophy of Biology series—free to download until October 6! Walter Veit examines the role of models in evolutionary biology: their types, testability, and interrelations 👇📕 www.cambridge.org/core/element... #philsci #HPBio #evobio
- Reposted by Charlie PyleReally interesting work from our neighbor Clare Smith @dukemedschool.bsky.social starting from CC mice and linking Ctsz to TB infection outcome as well as functional variation in human populations (UW, Case Western, Uganda) and expression in human granulomas (@charliejpyle.bsky.social)
- Reposted by Charlie PyleWatch @maxgg.bsky.social, Group Leader at the Crick, as he explains what happens to cells that are infected with tuberculosis and how his lab is studying our bodies’ response to this infection. www.youtube.com/watch?v=VWCt...
- You can now explore the mRNA expression of your favorite TB gene of interest in our recently published human pulmonary M.tb granuloma single-cell dataset at the Broad Single Cell PORTAL! @broadinstitute.org @wallacewly.bsky.social singlecell.broadinstitute.org/single_cell/...
- Reposted by Charlie PyleSymptomatic primary EBV infection generates B cell subsets that gain access to the CNS, attract T cells and thereby initiate multiple sclerosis @nature.com www.nature.com/articles/s41...
- Reposted by Charlie PyleA review emphasizes the advantages of using zebrafish in research, outlines key differences in experimental approaches, and offers guidelines for designing studies in a way that enhances experimental rigor and reproducibility. www.nature.com/articles/s42...
- Reposted by Charlie PyleChoose Science. Choose Europe. A new Marie Skłodowska-Curie Actions Postdoctoral Fellowships 2025 call is now open. With a budget of €404.3 million, it will support around 1,650 researchers from Europe and beyond. Apply by 10 September → europa.eu/!fBTMgF
- Reposted by Charlie PyleWhen a person is diagnosed with #tuberculosis, one of their first questions is often, “Why did I get tuberculosis? Why me?” 💬 Explore a Seminar which aims to help healthcare providers identify those at risk of TB & address patient concerns: tinyurl.com/zb2bwces #MedSky
- Reposted by Charlie PyleResearchers pinpoint the genes responsible for the final three pea traits studied by Gregor Mendel more than 160 years ago go.nature.com/3EC3eqT
- Reposted by Charlie PyleToday is a great day to highlight what an incredible, extensive, and diverse learning resource MIT OpenCourseWare is! 🧪 #AcademicSky 🔗 ocw.mit.edu
- Reposted by Charlie Pyleborn too late to get a two Western blot Nature paper, born too early to be a science TikTokker, born just in time to start a postdoc during a global pandemic and look for faculty positions during a historic hiring freeze
- Reposted by Charlie PyleInspiring speakers highlighting the importance to our collective health as well as economic return on investment of basic, curiosity-driven research at yesterday’s @standupforscience.bsky.social Frightening to see active dismantling of decades of public investments in our health and futures.
- Reposted by Charlie PyleNancy By Ernie Bushmiller February 26,1954
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