Josh Riback
Assistant professor @BCMHouston. Interested in IDPs, RNPs, and condensates in cells viewed from biophysical chemistry and polymer biophysics. www.RibackLab.com
- Check out this fun thread on the visual story of #RibackLab 's collaborative work with #Goodell_Lab. Excited to see it in print today in @cp-cell.bsky.social. Thanks again to all team members, especially @gandhardatar.bsky.social and @sciencyelmira.bsky.social .
- Today's the print release of a #RibackLab and #GoodellLab collaboration— we’re excited to share the visual story behind the science, including the dramatic artwork that inspired how we thought about aberrant function of NPM1c - which occurs in about 1 in 3 adult acute myeloid leukemias (#AML).
- It’s hard to imagine the lab without you @evdokiiap.bsky.social. As my first postdoc, you built the cell-biology foundation and pushed into new biophysics to reveal how nucleolar thermodynamics is regulated. Can’t wait for your manuscript to be released. Wishing you the best in what’s next!
- Last week marked the end of my postdoc at #RibackLab. I spent three exciting and productive years at BCM, but it’s now time for the next chapter. Thank you to @superscijew.bsky.social for being a great mentor and to my colleagues for all the fun and science we shared together!
- Setting up tomorrow’s phase transition. Holiday lab party imminent! #RibackLab #CellularPhysicalChemistryGroup
- Reposted by Josh RibackNew publication! How to read the curves in biomolecular phase diagrams! A collaboration between the Schmit Group and Jonathon Ditlev, Les Loew, and @ani-chattaraj.bsky.social. 1/7 pubs.acs.org/doi/10.1021/...
- Reposted by Josh Riback🧪 Geometry beats chemistry: we show that self-assembly can be controlled by tuning large-scale geometric parameters rather than molecular binding energies. PNAS: doi.org/10.1073/pnas... Video summary: www.youtube.com/watch?v=6FS3... #SelfAssembly #Biophysics #PhysicsOfLife
- Reposted by Josh RibackOur review on the physics of phase separation in cells has been published by Rep. Prog. Phys. 🎉 doi.org/10.1088/1361... We hope that the text and citations are helpful for anyone interested in physical descriptions of condensates in cells!
- We wrote a review on the "Physics of droplet regulation in biological cells": arxiv.org/abs/2501.13639 Beside the basic #physics of phase separation, we discuss three aspects that separate cellular from traditional droplets:
- Thank you @leukemiaresearch.bsky.social ! This discovery would not have been possible without your support!
- Reposted by Josh RibackNow online! Disparate leukemia mutations converge on nuclear phase-separated condensates
- Reposted by Josh RibackScientists uncover nuclear droplets that link multiple #leukemias revealing new #therapeuticTarget. @gandhardatar.bsky.social @sciencyelmira.bsky.social @goodell-lab.bsky.social @superscijew.bsky.social et al @cellcellpress.bsky.social @bcmhouston.bsky.social www.bcm.edu/news/scienti...
- Reposted by Josh RibackThrilled to share that my third year as a postdoc in the Goodell Lab at BCM begins with a paper published in @cellpress.bsky.social. Grateful for an opportunity to be a part of this incredible multidisciplinary project.
- (1/10) How do diverse leukemia mutations converge on the same molecular program? In #RibackLab first manuscript @cp-cell.bsky.social, collaboration with @goodell-lab.bsky.social shows that disparate mutations rewire shared protein networks to form nuclear condensates called C-bodies.
- (1/10) How do diverse leukemia mutations converge on the same molecular program? In #RibackLab first manuscript @cp-cell.bsky.social, collaboration with @goodell-lab.bsky.social shows that disparate mutations rewire shared protein networks to form nuclear condensates called C-bodies.
- (2/10) We found that disparate drivers of acute myeloid leukemia (AML) – including NPM1c, KMT2A-r, and nucleoporin oncofusions – form nuclear condensates with a shared set of proteins including XPO1 and MENIN to drive leukemic gene expression (e.g. HOXA). www.cell.com/cell/fulltex...
- (3/10) We focused on NPM1c – the most common driver of AML – which is aberrantly exported to the cytoplasm. Endogenous protein tagging showed NPM1c in nuclear condensates distinct from WT NPM1, despite few amino acid differences.
-
View full thread(10/10) Thank you to everyone in the #RibackLab and @goodell-lab.bsky.social lab, especially to @gandhardatar.bsky.social and @sciencyelmira.bsky.social for this amazing work, and to NCI, CPRIT, @leukemiaresearch.bsky.social, Searle Scholars, and Ted Nash Long Life Foundation.
- Reposted by Josh RibackPatrick's paper is finally out, a label-free method to measure the composition of multicomponent biomolecular condensates!
- New findings out today in Nature Chemistry from the Brugués @brugueslab.bsky.social at PoL, alongside colleagues at @mpi-cbg.de and the IPF Dresden 🧪 The authors have discovered a new method to identify the molecular makeup of biomolecular condensates Read our press release here: tud.link/ajwtaz
- BRISKET IN - 24 hours to the summer lab party!!! #RibackLab #CellularPhysicalChemistryGroup
- 12 hours brisket wrapping at 4AM!
- Brisket served! Look at that smoke ring; Physical Chemistry is in action!
- Reposted by Josh RibackHow do chromatin remodelers use #IDRs to find TF binding partners? In our new Molecular Cell paper, we show that β-catenin is an adaptor that links SWI/SNF (cBAF) subunit ARID1A with binding partners via IDR-domain interactions. www.cell.com/molecular-ce...
- Reposted by Josh RibackOur new preprint 🧬🔗 www.biorxiv.org/content/10.1... BRD4-NUT forms liquid-like condensates that locally constrain nucleosomes via BRD4-mediated crosslinking— physical control of #chromatin by #LLPS transcription condensates. @semeigazin.bsky.social @katsuminami.bsky.social @masaashimazoe.bsky.social
- Reposted by Josh RibackVery excited to share our latest work in #MolCell showing NPM1 stabilizing the association of nucleolus associated domains (NADs) and recruiting G9a to establish their repressive chromatin states. www.sciencedirect.com/science/arti...
- Reposted by Josh RibackI am excited to share the latest paper from my lab where we leverage the selection bias of condensate-promoting oncofusions to uncover molecular rules governing condensate specificity and function. www.cell.com/cell/fulltex....
- Reposted by Josh RibackNow published! Big congrats to first author @gginell.bsky.social We are actively working improving/updating various aspects of FINCHES; don't hesitate to reach out if you run into issues, have questions. www.science.org/doi/10.1126/...
- Reposted by Josh RibackStm1 regulates Ifh1 activity revealing crosstalk between ribosome biogenesis and ribosome dormancy: Molecular Cell www.cell.com/molecular-ce...
- Reposted by Josh RibackA Perspective by @stephanieaw.bsky.social and @fraserlab.com discusses ways macromolecules use conformational entropy to control binding, catalysis, and allostery www.nature.com/articles/s41...
- Reposted by Josh RibackNew(ish) preprint! 📰What whole-cell patterns emerge from the arrangement, morphologies, and interactions of organelles in the 3D space of the cell? What would we see if we could gather ALL the whole-cell reconstruction data that's out there in one place? www.biorxiv.org/content/10.1... 🧵 1/11
- Reposted by Josh RibackExcited to share the @goodell-lab.bsky.social News&Views on 3 exciting studies by Vassiliou Lab, Chan Lab, and @trowbridgelab.bsky.social in @nature.com and @natcomms.nature.com, showing that #DNMT3A mutant (major driver of Clonal Hematopoiesis) blood cells depend on mitochondrial respiration.
- In lab’s #FIRSTPREPRINT, we present methods to measure nanometer-scale organization around & between specific proteins in condensates in live cells. We uncover unexpected heterogeneity for a liquid-like phase with local meshwork spanning 10-50nm, stemming from ribosome biogenesis in the nucleolus.
- Nanometer condensate organization in live cells derived from partitioning measurements biorxiv.org/content/10.1101/202…
- How? We design 150 protein probes and use thermodynamic principles to interpret their partitioning. In method (1), "Local Size Exclusion," variable-length amino-acid chains are attached to nucleolar proteins, expelling them based on their local mesh size. Basic principles allow precise distances.2/4
- In method (2), "Length-Dependent Spatial Coupling," two proteins are linked by variable-length chains to evaluate their spatial preferences. We determine that scaffold protein NPM1 is positioned 10nm away from ribosome biogenesis, facilitating condensate formation without impairing function. 3/4
- What nanometer structure-function relationships exist in your favorite condensate? We hope you will try to measure it! Congrats to Kris, @evdokiiap.bsky.social, Abby, Archish, Gandhar, and @schmitbiophysics.bsky.social . 4/4
- Reposted by Josh RibackCALVADOS-RNA is now published doi.org/10.1021/acs.... This is a simple model for flexible RNA that complements and works with the CALVADOS protein model. Work led by Ikki Yasuda who visited us from Keio University. Try it yourself using our latest code for CALVADOS github.com/KULL-Centre/...
- Reposted by Josh RibackCuts in NIH research grants will have a lasting negative impact on science and medicine in the U.S. Grants will continue to fund specific research costs, but essential infrastructure like labs, advanced equipment and support staff are crucial for groundbreaking work.
- Reposted by Josh RibackOur #CellLineOfTheWeek is a 3-for-1 and fluoresces fibrillarin, nucleophosmin, and dCas9-KRAB! 🦀🧫 Our cell lines & plasmids are available for just the cost of shipping to support discovery: www.allencell.org/cell-catalog... 📦 Distributed by @coriellinstitute.bsky.social & @addgene.bsky.social
- Reposted by Josh RibackGreat article (in which I am quoted) about biomolecular condensates, the cellular phenomena I work on in my day job! 🧪 www.scientificamerican.com/article/myst...
- Reposted by Josh RibackThis is a textbook example (condensates are only just reaching textbooks!) of why condensates matter, and how they tend to work. Here these liquid-like blobs create a microenvironment in the nucleus of cancer cells within which chromatin sustains a conformation accessible to transcription...
- Reposted by Josh RibackMicroscopy Nodes is now up on bioRxiv! 🚀 This is a Blender extension that seamlessly integrates and visualizes 3D microscopy data (TIF & @zarr.dev). High-quality volume rendering for anyone, in both EM and fluorescence, regardless of computational expertise! 🔬 www.biorxiv.org/content/10.1...
- Reposted by Josh RibackCurious how biomolecular condensates can localize ions? Using NMR, we found that condensates selectively bind and localize both chaotropic anions and kosmotropic cations. This alters the condensate composition, interface potential and RNA duplex stability inside. By @irissmokers.bsky.social
- Reposted by Josh RibackmRNA display continues to astound. Fantastic collaboration with Alex Buell, led by the peerless @rasmusnorrild.bsky.social. ~100,000 peptides and their propensity to join the phase separation of a model disordered protein. doi.org/10.1101/2024...
- Nice to see CALVADOS agrees with the nu (0.51) from their data fit with the MFF! www.nature.com/articles/s41...
- Reposted by Josh Riback
- Reposted by Josh RibackMeet the CALVADOS RNA model Ikki Yasuda, Sören von Bülow & Giulio Tesei have parameterized a simple model for disordered RNA. Despite it's simplicity (no sequence, no base pairing) we find that it captures several phenomena that depend on the charge, stickiness and polymer properties of RNA 🧬🧶🧪
- Reposted by Josh RibackCulture of mouse Embryonic Stem Cells, stained with SPY555-DNA from @spirochrome.com and recorded on an iSIM scope from @visitech.bsky.social. With ultra-sensitive Hamamatsu ORCA-Quest and recent Large Field-of-View upgrade, we can detect mitosis in 3D, for almost an hour, with 1-min resolution.
- 🎉 Thrilled to share my lab's first PhD defense! Huge congrats to Gandhar Datar and co-advisor Peggy Goodell! 🎓🔬 Gandhar's work in biophysics and leukemia led to the discovery of a new phase-separated condensate that drives leukemias. Stay tuned for more insights!
