Juan C. Landoni 🔬
Mitochondriac 🔬 Postdoc @EPFL Manley lab of Experimental Biophysics 🧪 Ph.D. @HelsinkiUni 🇦🇷🇫🇮🇪🇺🇨🇭🏳️🌈 (he/él)
jclandoni.github.io
- Reposted by Juan C. Landoni 🔬Organelles do NOT have a single uniform pH. And if you think they must, because “protons diffuse fast,” this paper is for you. A thread on why that assumption is wrong; and what we found instead. 🧵 1/n
- Organelles harbour pH gradients biorxiv.org/content/10.64898/20…
- Reposted by Juan C. Landoni 🔬🎸Why this is exciting!!🦖 Quantitative super-resolution often requires genetically encoded tags to get labeling sufficient for quantitative imaging. Here with qExM we use commercial antibodies for endogenous targets. This opens HUGE opportunities to count in tissues, organisms, or clinical samples!
- Reposted by Juan C. Landoni 🔬We then used qExM to measure the abundance of mitochondrial respiratory chain complexes in situ! We measured the abundances for Complex I, III, and IV. How they differ in absolute abundances in metabolically distinct subpopulations And even how T-cell activation changes supercomplex density
- Reposted by Juan C. Landoni 🔬How does qExM work? We use cryo-fixation + GMA anchoring to boost antibody labeling efficiency, then apply capture-recapture statistics to dual-labeled samples. The expansion makes epitopes more accessible while reducing label size relative to targets - perfect for quantitative imaging!
- Reposted by Juan C. Landoni 🔬We validated qExM first on nuclear pore complexes, attempting to quantify the 8 fold symmetry without using this prior knowledge. We achieved a mean error of just 9.4% 😍🔬🎸 With these tools in place we moved to tackle protein complex abundances that were unknown....
- Reposted by Juan C. Landoni 🔬🔬🚨New preprint alert! 🚨🔬 We developed quantitative expansion microscopy (qExM) - a method to accurately count proteins in situ by combining expansion microscopy's improved labeling with statistical estimators borrowed from ecology www.biorxiv.org/content/10.6... #SuperResolution #CellBiology
- #Mitochondria or Alien script? 🧪🔬👽
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Accidentally festive #Celegans gonad while trying out some awesome LUTs by @kwolbachia.bsky.social for #FluorescenceFriday 🧪🔬
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Preprint alert 🚨 Ever wondered what role fast #mitochondria trafficking plays in the simple cells we mostly use? In @julius-w.bsky.social et al. we use mitodynamics + simulations to identify the importance of outwards transport to distribute new material and keep network balance! Link below!
- Tune in! 🔬
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- It's not #FluorescenceFriday but unexpectedly beautiful data should always be celebrated 💚 #PKMO -stained #mitochondria cristae in #STED
- Another year and another group of brilliant young students preparing for the International Biology Olympiad #IBO 💪☘️ Thank you for making teaching so fun and inspiring, bon courage à tous, and most importantly: enjoy it!
- Smartest booth luring strategy I've seen: a pro barista on site 🤯 Drop the free pens. Science runs on espresso ☕️
- That's a wrap on a much-needed family vacay, thank you Costa Rica for your incredible nature 🌋🦥🏞️🐠🐡🐒🦈, now back to our regularly scheduled programming 🔬
- #Writing with a view 🤩
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Still buzzing from the incomparable @embl.org #SeeingisBelieving conference! 🔬 Great science and truly amazing people, thanks everyone! #EESImaging
- Who's coming next week? 🤩🔬 #EESImaging #SeeingisBelieving
- Reposted by Juan C. Landoni 🔬How do #stemcells integrate information to coordinate fate decisions? Delighted to finally see our work showing how growth factors regulate the mechano-osmotic state of the #nucleus and #chromatin to control #pluripotency exit out! www.nature.com/articles/s41... see 🧵 👇
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- "Who needs a 30x zoom anyway?" I just found out the answer 🌙🤩
- Proof that I also care about other organelles 😁🔬 🟢 #ER 👉👈 #Mitochondria 🟣
- Trying my hand at HTML/CSS using @github.com hosting for a new personal website 💻 Highly recommend! jclandoni.github.io Comments/tips welcome (should look nicer on desktop but mobile should work too).
- Still not a bean 🫘 #Mitochondria (and friends) in #retina cells 👀 EM tomography 🔬🧪
- So excited to follow the opening symposium of #SWAN 🦢[Sustainable Wellbeing Across Lifespan] at @helsinki.fi today! Congrats to @awartiovaara.bsky.social and all members for the exciting ongoing and future reseach! #mitochondria #metabolism #aging #health
- Order, chaos & complexity #Mitochondria in #muscle cells 💪🔬, color-coded by 3D depth 🧪
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- In case you haven't heard, #Argentinian scientists are live-streaming their deep-sea mission with real-time interactive commentary. It's genius, mesmerising, and triggering huge public interest and support for #science funding 🐙🧪 And the memes are great! #CONICET #MarDelPlataCanyon
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Is there still a reason for dumping figures at the end of manuscripts instead of in-text, even for #BioRxiv preprints? My scroll-finger is exhausted... 🙏🧪📜
- Want to capture elusive events without stressing out your cells? Check out @wl-stepp.bsky.social hybrid-EDA! A smart microscopy approach to detect events in phase, and only image when it matters! 🔬🧫🧪 www.biorxiv.org/content/10.1... @sulianamanley.bsky.social @maweigert.bsky.social
- Reposted by Juan C. Landoni 🔬A key insight was building temporal information and memory into the ANN architecture. A huge thanks to all, especially our ‘smart’ (in so many ways) partner @maweigert.bsky. We could now detect local depolarization following constriction, using commonly used (bleachy TMRE) fluorescent biosensors.
- Reposted by Juan C. Landoni 🔬Our latest preprint www.biorxiv.org/content/10.1..., describes Willi Stepp’s project to make smart microscopy even gentler by doing event detection in phase contrast. We developed neural networks to detect mito-LD and mito-lysosome contacts, as well as mitochondrial pre-fission constrictions.
- Reposted by Juan C. Landoni 🔬Combining bacterial end joining with programmable nucleases, we induced mtDNA deletions in human cells at varying heteroplasmy and examined their metabolic and cellular effects. Simple tool to apply across any cell type and engineer pathogenic mtDNA deletions. www.sciencedirect.com/science/arti...
- The magic of the live #mito straight from the microscope 🔬 for #FluorescenceFriday - brought to you by #SYBRgold 🟢 and #PKMO 🟠 @spirochrome.com @carolinklose.bsky.social @lycasworks.bsky.social
- Fresh new paper alert 🚨 by @marcellharhai.bsky.social @jourdainlab.bsky.social et al. expanding the inventory of potential #mitodisease genes and a herculean deep-dive into FAM136A across models and human disease! 🧪🧫🐭👂 Really happy to see it out and to have played a small part 🔬 #mito #sciencesky
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Reposted by Juan C. Landoni 🔬Welcome immigrants from X interested in science to the friendlier skies of @bsky.app, as documented by a new @nature.com survey (but you already knew that 😉) "Bluesky is much better for science. There is much less toxicity, misinformation, and distractions." www.nature.com/articles/d41...
- Thank you for the awesome probe, and big shout-out to @lycasworks.bsky.social for the STED wizardry ✨
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- New #mito peeps keep joining! Don't miss out on their awesome stuff! go.bsky.app/J6Bu5fL
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Reposted by Juan C. Landoni 🔬Nucleoids are known to be regularly spaced along mitochondria, important for their inheritance and maintenance of the local proteome. We identified pearling, a Plateau-Rayleigh type instability described since the 1980s, to be responsible: t.ly/KKmGK. Collab with JLS lab @hhmijanelia.bsky.social
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Reposted by Juan C. Landoni 🔬Stoked to share TWO preprints on mitochondrial pearling! 🎉 We uncover how spontaneous #mitochondria pearling drives #mtDNA nucleoid distribution (doi.org/10.1101/2024...), and @gavsturm.bsky.social et al. dissect the biophysics behind it (doi.org/10.1101/2024...). Details below! 🔬🧵🧪
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Reposted by Juan C. Landoni 🔬[Not loaded yet]
- Reposted by Juan C. Landoni 🔬Mitochondria consist of networks of cylindrical tubes, right? Not necessarily! - in our new preprint, @gavsturm.bsky.social investigates how mitochondria transiently adopt a beads-on-a-string morphology www.biorxiv.org/content/10.1...
- And here's the thread by @gavsturm.bsky.social explaining their herculean and fascinating work on #pearling #biophysics!
- Reposted by Juan C. Landoni 🔬I’m ultra excited about our preprints on mitochondrial pearling. In two concurrent collaborative studies with @wallaceucsf.bsky.social, we investigated its consequences on mtDNA nucleoids (led by @jclandoni.bsky.social) & its biophysical causes (led by @gavsturm.bsky.social). Funders HFSP, ERC, SNSF
