Matt Lycas
Neuroscientist and Super-Resolution Microscopist
- Reposted by Matt Lycas🧪 We are excited to share this novel open access paper on Phasor Mixing Coefficient to analyze colocalization, developed by folks @i2janelia.bsky.social and @aicjanelia.bsky.social. This is also the first technical paper jointly published with our sister imaging center @malacridalab.bsky.social!
- 🔬🚨New preprint alert! 🚨🔬 We developed quantitative expansion microscopy (qExM) - a method to accurately count proteins in situ by combining expansion microscopy's improved labeling with statistical estimators borrowed from ecology www.biorxiv.org/content/10.6... #SuperResolution #CellBiology
- Reposted by Matt LycasFound the unbroken link: www.biorxiv.org/content/10.6...
- Reposted by Matt Lycas@lycasworks.bsky.social developed quantitative expansion (qExM) to estimate endogenous protein quantities in situ. His ExM protocols offer epitope preservation and accessibility so that standard Western Blot antibodies can be used. Also, check out the cristae, supercomplexes, specialized mitos 🤩
- 🔬🚨New preprint alert! 🚨🔬 We developed quantitative expansion microscopy (qExM) - a method to accurately count proteins in situ by combining expansion microscopy's improved labeling with statistical estimators borrowed from ecology www.biorxiv.org/content/10.6... #SuperResolution #CellBiology
- Reposted by Matt LycasPIs & Group Leaders, do your trainees need to learn light microscopy and image analysis? Send them to us! Financial aid is available! Applications due Friday 1/30. meetings.cshl.edu/courses.aspx...
- Reposted by Matt LycasI finally got around to writing the discussion section of our lab's basic training course on live cell #microscopy. I explain the tradeoffs involved in designing an imaging experiment and the art of thinking of the experiment as an optimization problem. leb-epfl.github.io/basic_traini...
- Reposted by Matt Lycas🔬 🖥️ Applications are open for the CSHL course Quantitative Imaging: From Acquisition to Analysis (April 6–21, 2026)! An intensive, hands-on course covering advanced fluorescence microscopy and quantitative image analysis using open-source tools. 🗓️ Apply online by Jan 30, 2026
- Seal taking a nap off the coast of Cape Town
- Reposted by Matt LycasA super detailed protocol + video on Cryo-ExM - Cryo-Expansion Microscopy, led by the labs of our former postdocs @marinelap.bsky.social & @ebertiaux.bsky.social. Clear, practical, and very useful for anyone doing nanoscale imaging 🚀 app.jove.com/t/68595/expa...
- Reposted by Matt Lycas✨ Blinking #nanobodies that work for single-molecule localization 🔬 Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!
- Reposted by Matt LycasThrilled to see our review article "The evolutionary origins of synaptic proteins" highlighted on the cover of Nature Reviews Neuroscience 🤩. www.nature.com/articles/s41... @msarscentre.bsky.social 🧠✨🧬🌊🪼🧽
- First neurons didn’t appear overnight. We trace their roots to ancient secretory cells - showing how lifestyle & behavior shaped the evolution of first synapses.🧠🌊 #Evolution #Neuroscience Our latest in @natrevneuro.nature.com Link: rdcu.be/eMX3E @jeffcolgren.bsky.social @msarscentre.bsky.social
- Reposted by Matt LycasNew preprint showing how the membrane-associated periodic skeleton (MPS) restricts endocytosis in neurons: www.biorxiv.org/content/10.6... It nicely confirms our work from last year (science.org/doi/10.1126/science.ado2032) and extends it to other compartments in more mature neurons
- Reposted by Matt LycasNew paper from @zhixingchen2.bsky.social's lab! It turns out that, in addition to its very low phototoxicity, PKmito Deep Red (PKMDR) directly reports on mitochondrial membrane potential (MMP) in live cells through its lifetime! www.nature.com/articles/s41...
- FLIM at the Biozentrum 🔬
- Tardigrades looking BIG
- Reposted by Matt LycasWe are highlighting Jennifer Waters, a @focalplane.bsky.social Scientific Advisory Board member, Director of CITE, Harvard Medical School, and creator of MicroList (now featured in FocalPlane) and Microtutor, as an extraordinary biologist. #100biologists @jencwaters.bsky.social
- Expansion microscopy of a tardigrade I caught outside with the objective I got on eBay
- Bought more objectives on eBay
- Putting the eBay objective to good use
- Super resolution is super T cell Mitochondria labelled for MT-CO1
- Shout out eBay, part 1 of the home microscope
- Tardigrades in the mail
- Silver metal at Swiss BJJ Championship
- So happy to be at #GEF25 thank you to the organisers and I can’t wait for all our future developments in expansion microscopy
- #GEF2025 Such a fantastic conference 🥳🥳🥳
- More Iterative expansion for a specific inner mitochondrial membrane protein
- Iterative expansion microscopy for protein on the mitochondria inner membrane
- A specific protein *
- Great Cormorant, Preverenges Switzerland. Go birds
- Happy Physio!
- Go Physio!!!
- Happy Physio!
- New tattoo: Cajal‘s microscope. His illustrations of cortical neurons, hippocampal neurons, synapses, and the brain. Shout out to Phil from the tattoo Lab for his brilliant work
- Cristae
- Mitochondria!
- Phase! Contrast!
- Just testing out DIC on my cheek cells for a writing break
- Reposted by Matt Lycas
- Happy Expansion Microscopy Day at Quantitative Imaging @qiatcshl.bsky.social
- Reposted by Matt LycasOur latest preprint www.biorxiv.org/content/10.1..., describes Willi Stepp’s project to make smart microscopy even gentler by doing event detection in phase contrast. We developed neural networks to detect mito-LD and mito-lysosome contacts, as well as mitochondrial pre-fission constrictions.
- Great imaging today at Quantitative Imaging at CSHL @qiatcshl.bsky.social #expansion
- Expansion microscopy of a tardigrade
- Demonstrating how local environment impacts fluorescence 🔬🧪🥼@qiatcshl.bsky.social
- Reposted by Matt LycasThe final version of our work on the in-cell architecture of the mitochondrial respiratory chain is now online in @science.org 🎉 You can find the full story here www.science.org/doi/10.1126/... @verenaresch.bsky.social did a wonderful job on the cover and animation, she really brought it to life 👩🎨
- In a new Science study, cryo–electron tomography captures the in-cell architecture of the mitochondrial respiratory chain, illuminating how the coordinated action of molecular machines drives life’s fundamental energy conversion. Learn more in this week's issue: scim.ag/3FA3Ygq
- Reposted by Matt LycasIf you are looking to do GPU deconvolution in Fiji checkout the new updates to CLIJ (OpenCL for ImageJ) deconvolution. @nkiaru.bsky.social has done updates to the tiled deconvolution and integrated with BigDataViewer, and I've added a new script for batch processing. forum.image.sc/t/clij-openc...
- Reposted by Matt Lycas@science.org A subcellular map of translational machinery composition and regulation at the single-molecule level | Science www.science.org/doi/10.1126/...
- Fluorescence Freitag!
- Tardigrade
- Tardigrades and Rotifers
