Florent Waltz
SNSF Ambizione Project Leader
Looking at tiny frozen mitochondria with big microscopes 🔬 cryoEM/cryoET
Green organisms 🌿 & evolution 🦠
Biozentrum, hosted in the Engel Lab, CH
🇫🇷 -> 🇩🇪 -> 🇨🇭
Website: waltzresearch.com
- Reposted by Florent WaltzFor #ECR’s: DBG’s Hot Tpoic Workshop on Cryo-Electron Tomography + FIB-milling 13 – 15 April 2026 In @cellarchlab.com 's lab @biozentrum.unibas.ch Organised by @tamb-o.bsky.social, Karen Zinzius, @fannyleblanc.bsky.social Details: www.deutsche-botanische-gesellschaft.de/en/about-us/...
- Reposted by Florent WaltzAm I the only one increasingly annoyed by cryo-EM papers not showing *any* experimental density in figures? Sometimes not even in the supplementary material??
- Check this new study on carbon concentration mechanisms! See how the pyrenoid changes by modifying Rubisco linkers in our favorite alga, Chlamy 💚! My desk mate @phaips.vd.st spent a lot of time collecting & carefully analyzing these beautiful tomograms 🔬. Congrats to all the authors!
- How does molecular valency shape condensate assembly and function? We used the CO2-fixing organelle in algae—the pyrenoid—to find out… 🧵 Preprint here!: www.biorxiv.org/content/10.6... @cellarchlab.com @phaips.vd.st @biologyatyork.bsky.social #Rubisco #PhaseSeparation #Condensates #Pyrenoid
- Reposted by Florent WaltzYou like #LLPS? #TeamTomo? #Rubisco? Check out our latest preprint! What a great collaboration this has been!
- How does molecular valency shape condensate assembly and function? We used the CO2-fixing organelle in algae—the pyrenoid—to find out… 🧵 Preprint here!: www.biorxiv.org/content/10.6... @cellarchlab.com @phaips.vd.st @biologyatyork.bsky.social #Rubisco #PhaseSeparation #Condensates #Pyrenoid
- Reposted by Florent WaltzThis one was quite the journey! The paper describing the #ChlamyDataset is finally out and on the cover of Mol Cell! This beautiful rendering made by co-author @jessheebner.bsky.social and Holly Peterson shows an instance of mitochondrial fission found in the dataset 😍 [Maybe long thread ahead]
- The latest issue is now online www.cell.com/molecular-ce...
- Reposted by Florent WaltzThe latest issue is now online www.cell.com/molecular-ce...
- Reposted by Florent WaltzOnline Now: Toward community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii Online now:
- Reposted by Florent WaltzJapan is joining Horizon Europe, the EU’s flagship research and innovation programme. Openness and international cooperation can shape a bright future for science and technology. Through science, we can build bridges, strengthen competitiveness, and accelerate the green and digital transitions.
- Reposted by Florent WaltzGiving the gift of negative data 🥰 Since I joined @lsajournal.org I have handled several stellar manuscripts that report null results. These papers do very well and reviewers almost always appreciate them! Thanks @rockefeller.edu news team for the article! www.rockefeller.edu/news/38829-t...
- Reposted by Florent Waltz
- Final version is out! Our large-scale cryo-ET dataset 🔬 of Chlamydomonas reinhardtii 🦠 is now published in @cp-molcell.bsky.social Huge collaborative effort! So glad to see the community already using it to develop new resources & tools. Check it out here: shorturl.at/z4i4c #CryoEM #CryoET
- If you want to learn how to process cryo-ET data using the Chlamy dataset don't forget to check : tomoguide.github.io @phaips.vd.st
- And one more story 🎩 and surprise 📔 to come in January, so stay tuned.
- Reposted by Florent WaltzStructural insights into cauliflower mitoribosome in translation state and in association with a late assembly factor pubmed.ncbi.nlm.nih.gov/41330901/ #cryoEM
- Reposted by Florent WaltzPreprint alert! It is my great pleasure to announce the first manuscript from the lab, a story that started @gmivienna.bsky.social and was mainly accomplished by the intrepid @gesahoffmann.bsky.social at @mpi-mp-potsdam.bsky.social. A brief thread with our findings www.biorxiv.org/content/10.1...
- Yeah super cool! Look at these beautiful cells, tons of eukaryotes 🦠 images using expansion microscopy 🔬 What's next? Expand the world 🌎 ?! Congrats !
- 🚨Our collaboration with @centriolelab.bsky.social & @gautamdey.bsky.social is out today in @cp-cell.bsky.social We show that #Expansion #Microscopy is a broad-spectrum modality for Euks, enabling 3D phenotypic maps rooted to phylogeny. #ProtistsOnSky #SciComm #SciSky www.cell.com/cell/fulltex...
- Reposted by Florent WaltzWe’re back with the next DinoSphere Online Seminar! Join us on Nov 4th, we're hosting: Edmée Royen (ULiège) - Symbiodinium Nicolas dos Santos Pacheco (Cambridge) - Perkinsids 📅 Tue, Nov 4 - 4 PM CET/3 PM GMT 💻 Zoom link: sites.google.com/view/dinosphere Please spread the word! #protistonsky
- Last Friday (17th), we had an amazing first Young Swiss Tomo meeting in Bern, organized with Elda Bauda, Zanetta Kechagia, and @leonieanton.bsky.social! Over 70 people from 🇨🇭 gathered to talk about cryo-electron tomography 🔬. Huge thanks to the DCI for making the event possible! See you next year! 👋
- Reposted by Florent Waltz✨Excited that the main project of my PhD is now available as a pre-print on #bioRxiv Here, we used #CryoET to visualise mitochondrial proteostatic stress and together with SPA #CryoEM shed light into the functional cycle of the Hsp60:10 chaperone system. #TeamTomo 🔗 www.biorxiv.org/content/10.1...
- Check this out! The mysterious widespread CAGE protein complex found by my colleague @computingcaitie.bsky.social ! Cool stuff there 🥨 Shows you how much we still can and have to discover !
- I am excited to share our new preprint on the CAGE complex, a mysterious hollow protein complex that I first saw years ago while surveying Tetrahymena ciliary lysate www.biorxiv.org/content/10.1... #cilia #protistsonsky 🧬🧪
- I had a wonderful time in Japan 🗼! Huge thanks to @ken-wakaba.bsky.social for inviting me to Sangyo University in Kyoto. Thanks to Takashi Yamano (and Ken-ichi) for organizing the Beyond the Green 🍀 session at the Japanese Botanical Society meeting! Great science and discussions!
- Mitochondria finally made the headlines, yay! Oh wait, ... oh no ....
- via @acyn.bsky.social RFK JR: I’m looking at kids as I walk through the airports today...and I see these kids that are just overburdened with mitochondrial challenges, inflammation—you can tell from their faces, movements, and lack of social connection
- Reposted by Florent WaltzOur #CryoET story on how a top antimalarial drug candidate perturbs the native malarial translation machinery is out @natsmb.nature.com🥳 rdcu.be/eBbrH A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo
- Very cool! How do cytosolic ribosomes discriminate between ER and mitochondria targeted proteins? NAC recognizes mitochondrial targeting sequences! Very important since over 95% of the mitochondrial proteome is translated in the cytosol and then imported to mitochondria. Congrats!
- Check out our new preprint on the discovery of a molecular switch in NAC that mediates nascent chain sorting on the ribosome and prevents mitochondrial protein mistargeting by SRP. A great collaboration with the Shan Lab @Caltech and the Qi Lab @UVA: www.biorxiv.org/content/10.1...
- A step towards easier control over mitochondrial gene expression: Silencing mitochondrial gene expression in living cells | Science www.science.org/doi/10.1126/...
- Cell-to-cell connections in plants 🌿! Using cryo-ET 🔬 to have a look at plasmodesmata. Congrats Marcel, Matthias and co! You managed to push it far beyond what I thought was possible !
- New preprint: In situ Architecture of #Plasmodesmata. Using #cryoET, #AlphaFold & proteomics, we uncover the native organization of plant intercellular nanopores. 🔗 doi.org/10.1101/2025... 🧪🧵1/n #teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM
- Reposted by Florent WaltzNew preprint: In situ Architecture of #Plasmodesmata. Using #cryoET, #AlphaFold & proteomics, we uncover the native organization of plant intercellular nanopores. 🔗 doi.org/10.1101/2025... 🧪🧵1/n #teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM
- Cool paper from cool people 😎 Expand all the things!
- Excited to share the lab's 1rst preprint! Rapid high-res immunofluorescence is now possible in cultured & environmental diatoms thanks to 4-fold expansion microscopy. A step-change for comparative cell biology in one of the most important phytoplankton groups on the planet 🥳 tinyurl.com/m9s5su7s 1/2
- Curious about dinoflagellates? Tiny water bugs with super cool cellular architecture? Check out this cool initiative from my colleague @manondemulder.bsky.social and her friends! Their 1st online seminar is next month, don't miss it!
- We’re kicking off the DinoSphere Online Seminar Series! Join us for our first session with Karel Mockaer (Heidelberg) & Yong Heng Phua (OIST) 📅 1 July 9AM CEST 🔗 tinyurl.com/4mjaverj Spread the word! @protistwtmostest.bsky.social @ehehenberger.bsky.social @chandnibhickta.bsky.social&Norico Yamada
- Re-redeading this one 📖. Great book for evolution and bioenergetics aficionados. The view is a bit distracting though ⛵
- Reposted by Florent Waltz[This post could not be retrieved]
- Reposted by Florent Waltz
Happy to present the lab latest work, today from the green-side. The ultimate PSII assembly intermediate that directly precedes incorporation of the Mn4O5Ca cluster and formation of the OEC. Psb32 binds to PsbV, challenging the assumption the extrinsic subunits all associate spontaneously in-vivo. - Reposted by Florent Waltz“The Website” is here! It can be intimidating getting into analysis of #CryoET data, and software is evolving all the time👩💻🚀. @phaips.vd.st & @florentwaltz.bsky.social put our lab’s workflows into an online guide. We hope this helps lower the bar to joining #TeamTomo. Feedback much appreciated! 🧪🧶🧬🔬
- Hey #TeamTomo, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why @florentwaltz.bsky.social and I made this website! tomoguide.github.io Follow the thread 1 /🧵 #CryoET #CryoEM 🔬🧪
- You want to start tomography? Solve structures inside cells? Reach Nyquist 😳 ? @phaips.vd.st and I have a website for you! tomoguide.github.io You'll find a tutorial on how to reconstruct tomograms, pick particles and do subtomogram averaging, using different software! Hope it will be useful !
- Hey #TeamTomo, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why @florentwaltz.bsky.social and I made this website! tomoguide.github.io Follow the thread 1 /🧵 #CryoET #CryoEM 🔬🧪
- Reposted by Florent WaltzHey #TeamTomo, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why @florentwaltz.bsky.social and I made this website! tomoguide.github.io Follow the thread 1 /🧵 #CryoET #CryoEM 🔬🧪
- Reposted by Florent WaltzAlso want to highlight that the script is based on the awesome aretomo3torelion5 from @phaips.vd.st 🤩 github.com/Phaips/areto...
- Reposted by Florent WaltzTo all #ChlamyDataset enthusiasts, here is a script that lets you directly import the pre-processed results from EMPIAR-11830 into #RELION v5 for subtomogram averaging: github.com/Chromatin-St... #Chlamydomonas #TeamTomo #CryoET #OpenSoftwareAcceleratesScience
- Reposted by Florent Waltz1/5: Over the last few months, I have been working on an update for the #FollowRelionGracefully dashboard, which offers improved real-time job previews for #cryoEM #Relion jobs.
- Check out the latest version of MemBrain, spearheaded by computation superstar @lorenzlamm.bsky.social ! It can segment, pick particles and give you metrics on everything!
- 🦠🧠 MemBrain update! 🧠🦠 We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics. 🔗 Preprint: doi.org/10.1101/2024... 🔗 Code: github.com/CellArchLab/... 🧵(1/6) #TeamTomo
- Reposted by Florent Waltz🦠🧠 MemBrain update! 🧠🦠 We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics. 🔗 Preprint: doi.org/10.1101/2024... 🔗 Code: github.com/CellArchLab/... 🧵(1/6) #TeamTomo
- Reposted by Florent WaltzFrom my colleague @cellarchlab.com and his lab at the @biozentrum.unibas.ch I continue to be amazed by what cyro-electron tomography can do...
- Reposted by Florent WaltzCongratulations Florent and coauthors!!! I'm very excited to continue diving into diversity and beauty of mitochondrial respiratory complexes in algae 😍
- The final version of our work on the in-cell architecture of the mitochondrial respiratory chain is now online in @science.org 🎉 You can find the full story here www.science.org/doi/10.1126/... @verenaresch.bsky.social did a wonderful job on the cover and animation, she really brought it to life 👩🎨
- Reposted by Florent WaltzOut just now @naturecomms.bsky.social: using cryo-EM and cryo-electron tomography, we provide evidence that structurally heterogeneous ribosomes can cooperate in protein synthesis in bacterial cells: www.nature.com/articles/s41...
