Florian Wollweber
Imaging Asgard archaea and other strange organisms @embl.org Grenoble | 🦠❄️🔬#teamtomo
embl.org/wollweber
- Reposted by Florian Wollweber[Not loaded yet]
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- Reposted by Florian Wollweber🌀 How do spirochete bacteria swim through thick fluids like champions? We solved T. denticola flagella structure - asymmetric proteins expand one side, compress the other for perfect corkscrew motion! @debnathghosal.bsky.social 🔗 doi.org/10.64898/202... #StructuralBiology #CryoEM #Microbiology
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- Reposted by Florian WollweberI've been working on Surface Morphometrics for a new version (coming soon), and made some significant (AI-assisted) performance improvements, especially in pycurv (20-50x!). If you've had technical issues or just felt like it was too slow, give it another try! #teamtomo github.com/GrotjahnLab/...
- Reposted by Florian WollweberExtremophile alert! Registration is OPEN for the 2026 EMBO Archaeal workshop in Cambridge. Spaces are limited, so register soon with an abstract if you’d like to give a talk. If you can’t come in person, we are welcoming virtual attendees. So join us online where all talks will be screened!
- Sign up now for the EMBO Workshop "#MolecularBiology of #Archaea: Life Through the Prism of Archaea" in Cambridge, UK, 6–10 July 2026. Deadline: 15 April meetings.embo.org/event/26-archaea #EMBOArchaeaBiology #EMBOevents #CellBiology #StructuralBiology #ecology #evolution #biophysics 🧪
- Reposted by Florian WollweberThx for @mollyherring.bsky.social for featuring our recent work with @centriolelab.bsky.social and our quest with @gautamdey.bsky.social & the support of the @moorefound.bsky.social to look for the beauty in every #eukaryote out there using #Expansion #Microscopy #UExM
- Even with the largest zoom on a classic compound optical system, scientists struggle to make sense of finer details. Microbiologists are turning to an unexpected source to clear things up: a moisture-absorbing material found in diapers. www.quantamagazine.org/expansion-mi...
- Reposted by Florian WollweberWe are hiring for group leaders again — EBI is a great place to start your research group! embl.wd103.myworkdayjobs.com/EMBL/job/Hin...
- Reposted by Florian WollweberExcited to share this detailed biophysical study of #PhaseSeparation in the Pyrenoid, an algal microcompartment that fixes a third of the Earth's #CO2 🌏. We combine methods across scales, in vitro 🧪 and in vivo 🦠 Thanks for the very fun collaboration! 🤗 #PlantScience 🌾 #TeamTomo 🔬 #LivingPhysics 🧬🧶
- How does molecular valency shape condensate assembly and function? We used the CO2-fixing organelle in algae—the pyrenoid—to find out… 🧵 Preprint here!: www.biorxiv.org/content/10.6... @cellarchlab.com @phaips.vd.st @biologyatyork.bsky.social #Rubisco #PhaseSeparation #Condensates #Pyrenoid
- Reposted by Florian WollweberFoldMason is out now in @science.org. It generates accurate multiple structure alignments for thousands of protein structures in seconds. Great work by Cameron L. M. Gilchrist and @milot.bsky.social. 📄 www.science.org/doi/10.1126/... 🌐 search.foldseek.com/foldmason 💾 github.com/steineggerla...
- Reposted by Florian WollweberLove seeing the archaeal work at EMBL highlighted! 🥰 Feels great to be part of a growing community pushing this fascinating biology forward! #teamArchaea #EMBL 🧫🤓🧪🌏
- Archaea may not be well known, nor well studied, but these microorganisms can live in the most extreme environments, but also right on our skin. They’re everywhere. EMBL researchers are now exploring their unique ecosystem adaptability and link to evolution. www.embl.org/news/science...
- Reposted by Florian WollweberWhen you forget to turn on high-res interpolation in 3dmod
- Reposted by Florian WollweberArchaea may not be well known, nor well studied, but these microorganisms can live in the most extreme environments, but also right on our skin. They’re everywhere. EMBL researchers are now exploring their unique ecosystem adaptability and link to evolution. www.embl.org/news/science...
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- Reposted by Florian WollweberThe origin of eukaryotic cells continues to be one of the greatest mysteries in biology. EMBL Grenoble is excited to welcome Florian Wollweber, who will use cutting-edge imaging techniques to study Asgard archaea — microbes closely related to our earliest ancestors. 🦠🔬 www.embl.org/news/people-...
- Reposted by Florian WollweberNew on the CryoET Data Portal: ~27,000 tomograms of affinity-captured lysosomes from HEK293T cells across 4 physiological states. Includes raw data, AreTomo3 reconstructions & Membrain-Seg annotations. Openly available for reuse! cryoetdataportal.czscience.com/depositions/...
- Reposted by Florian WollweberSAVE THE DATE! The next instalment of the long-running Bacterial and Archaeal Cell Division Conference will take place at www.marriott.com/en-us/hotels..., Italy from the 20th to 23rd of April 2027. Organisers: me, @s-lab.bsky.social, @awehenkel.bsky.social. More info soon. #MicroSky
- Reposted by Florian WollweberA reminder to sign up for the 2026 EMBO archaeal meeting im Cambridge: meetings.embo.org/event/26-arc.... Registration is open. We are looking forward to hosting you!
- Reposted by Florian Wollweber🔬🚨New preprint alert! 🚨🔬 We developed quantitative expansion microscopy (qExM) - a method to accurately count proteins in situ by combining expansion microscopy's improved labeling with statistical estimators borrowed from ecology www.biorxiv.org/content/10.6... #SuperResolution #CellBiology
- Reposted by Florian WollweberThe @dmf-unil.bsky.social is supporting the application of a candidate for the SNSF/ERC Starting Grant (deadline 5th of May). Reach out to me if you are interested. We are collecting CV’s until the 8th of Feb. We can offer a stimulating scientific environment and help with the application/interview.
- Reposted by Florian Wollweber🔬🔦Our Imaging spotlight from @hiralshah.bsky.social & team highlights their U-ExM atlas of planktonic eukaryotes, including details on how you can explore this wonderful resource. Plus, check out Hiral’s top tips on getting started with expansion microscopy. focalplane.biologists.com/2026/01/20/i...
- Reposted by Florian WollweberLLOMe has long been used to study lysosomal damage, yet how it works has remained a mystery. Using cryo-electron tomography, we show it forms amyloid structures inside lysosomes that mechanically rupture membranes – revealing a new paradigm for lysosomal failure. 🔗 doi.org/10.64898/202... #CryoET
- Reposted by Florian Wollweber🔬 Postdoc position – Infection Biology & Cryo-ET (Zurich) 🇨🇭 We are looking for a motivated Postdoc to join our interdisciplinary team. 📩 Interested? Check out the job advert: www.imm.uzh.ch/dam/jcr:cb17... 👉 More info on the lab: www.weiss-laboratory.com Please share with interested colleagues!
- Reposted by Florian WollweberWe resolved the molecular architecture of a condensate—inside cells. 🤯 By combining in situ cryoET with multiscale simulations, we show how a single point mutation modifies molecular interactions, shifts the condensate’s material properties, and alters its biological function. Preprint 👇
- We are excited to share our new preprint which is now available to read on biorXiv: doi.org/10.64898/202... 🎉🎉🎉
- Reposted by Florian WollweberWe are excited to share our new preprint which is now available to read on biorXiv: doi.org/10.64898/202... 🎉🎉🎉
- Reposted by Florian WollweberOne of the best #TeamTomo studies of phase-separated condensates I’ve seen. I am reminded of Rubisco condensates (pyrenoids) for the ability to make such quantitative measurements of molecular organization. It really helps to have intrinsic ~500 kDa fiducials. Awesome mechanistic stuff!! 🧪 🧶🧬 🔬
- We are excited to share our new preprint which is now available to read on biorXiv: doi.org/10.64898/202... 🎉🎉🎉
- Reposted by Florian WollweberThis large-scale open dataset and the continued community development around it exemplify the spirit of #TeamTomo 🧪 🔬 👨🔬👩💻
- Excited to see this work on the cover of #MolecularCell. A massive, open #cryoEM #cryoET dataset capturing molecular organization inside intact cells, pushing what’s possible for #visualproteomics, method development, and computational reuse. Congratulations to the authors. ter.li/of4cgh
- Reposted by Florian WollweberWe are very excited to see this work out in its final form at Ultramicroscopy! Knife edge measurements of beam shapes and improved scanned versus shaped beam material removal comparisons. Find the OA article here: doi.org/10.1016/j.ul...
- Thrilled to share these experiments on shaping focused ion beams to explore different ways of milling. Here, we explore using different beam geometries to generate cellular thin sections/lamellae. The video shows elongating the beam on a charging spot burn www.biorxiv.org/content/10.1...
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- Reposted by Florian WollweberThe #ChlamyDataset is on the cover of @cp-molcell.bsky.social 🖼️🥰! Read @lifeonthewedge.bsky.social's great thread🧵 for the inside scoop🍨 on all the #TeamTomo developments already made possible since these 1829 tomos hit EMPIAR 🧪 🧶🧬 For more, here's the old preprint thread: bsky.app/profile/cell...
- This one was quite the journey! The paper describing the #ChlamyDataset is finally out and on the cover of Mol Cell! This beautiful rendering made by co-author @jessheebner.bsky.social and Holly Peterson shows an instance of mitochondrial fission found in the dataset 😍 [Maybe long thread ahead]
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- Reposted by Florian WollweberI'm so happy to have gotten the opportunity to work on this project and I'm SO excited that they selected our image for the cover!
- This one was quite the journey! The paper describing the #ChlamyDataset is finally out and on the cover of Mol Cell! This beautiful rendering made by co-author @jessheebner.bsky.social and Holly Peterson shows an instance of mitochondrial fission found in the dataset 😍 [Maybe long thread ahead]
- Reposted by Florian WollweberThis one was quite the journey! The paper describing the #ChlamyDataset is finally out and on the cover of Mol Cell! This beautiful rendering made by co-author @jessheebner.bsky.social and Holly Peterson shows an instance of mitochondrial fission found in the dataset 😍 [Maybe long thread ahead]
- The latest issue is now online www.cell.com/molecular-ce...
- Reposted by Florian WollweberMedina, Chang et al. of the @nanigrotjahn.bsky.social lab introduce a new method based on the Surface Morphometrics pipeline to measure global and local thicknesses of organellar #membranes from cellular cryo-electron tomography data. rupress.org/jcb/article/... #CryoET
- Reposted by Florian WollweberA new year brings new opportunities: we are looking to fill a permanent professorship position with a Microbiologist preferably with expertise on archaeal biology, RNA biology or imaging techniques to unravel cell biology in prokaryotes! Please share and/or apply :)! www.nature.com/naturecareer...
- Reposted by Florian WollweberThanks @jcellsci.bsky.social for this opportunity to contribute to your centenary collection with our take on the state of the field - 10 years after its modern reincarnation 🧪🌍 W/ @alebenoit.bsky.social @eelcotromer.bsky.social @fritzlaylin.bsky.social journals.biologists.com/jcs/article/...
- Reposted by Florian WollweberFinal version is out! Our large-scale cryo-ET dataset 🔬 of Chlamydomonas reinhardtii 🦠 is now published in @cp-molcell.bsky.social Huge collaborative effort! So glad to see the community already using it to develop new resources & tools. Check it out here: shorturl.at/z4i4c #CryoEM #CryoET
- Reposted by Florian WollweberJOB ALERT: Operations Director (DCI–Lausanne). The DCI, a joint initiative of EPFL, UNIL, UNIGE and UNIBE, is recruiting an Operations Director to lead the cryo-EM facility in Lausanne. 📩 Questions: CEO of the DCI: @chgenoud.bsky.social 🔗 Apply here: careers.epfl.ch/job/Lausanne...
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- Reposted by Florian WollweberThrilled to share the preprint from my PhD! 🥳 Where I characterised epithelial cell extrusion in a sea anemone 🔗 www.biorxiv.org/content/10.6... Big thanks to my supervisor @prhsteinmetz.bsky.social and coauthors @noahbruderer.bsky.social @lionlchristiaen.bsky.social at🇳🇴 @msarscentre.bsky.social ⬇️🧵
- Reposted by Florian WollweberAre you a long-time fan of Archaea, an extremophile-phile, or are you simply curious? Either way, we have good news. We’re delighted to announce the 2026 EMBO Workshop on Archaea, 6–10 July. Sign up: meetings.embo.org/event/26-arc... We look forward to seeing you in Cambridge, UK. Please repost!
- Reposted by Florian WollweberIt’s out! Using cryo-ET in Dicty cells, we take a fresh in situ look at vaults. Surprisingly, we uncover vaults associated with ER and NE membranes, and find that many vaults enclose ribosomes in defined orientations, opening new avenues to their cellular function! www.biorxiv.org/lookup/conte...
- The vault associates with membranes in situ biorxiv.org/content/10.64898/20…
- Reposted by Florian WollweberVaults. They are cell biology's greatest puzzle! This preprint from Martin Beck's lab shows them docked on ER membranes with a ribosome inside. What on earth is going on there?? #CellBiology #WTFology www.biorxiv.org/content/10.6...
- Reposted by Florian WollweberMicrobial eukaryotes keep challenging assumptions about eukaryotic cell and genome biology. Our upcoming workshop explores the frontier of protist genomics and how it shapes cell biology, ecology and evolution. Please save the date! Talks, posters and ECR events Websites and details coming soon
- Reposted by Florian Wollweber“Cultivation of Methanonezhaarchaeia, the third class of methanogens within the phylum Thermoproteota” by @kohtzarchaeota.bsky.social, Sylvia Nupp, and myself is out in Science Advances. 90% enriched culture of a methyl-dismutating thermoproteotal methanogen. #Microsky 🧪 tinyurl.com/bdcc3uzs
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- Reposted by Florian WollweberLove the caption of Fig. 3 in this impressive preprint: "A Goodsell-esque 3D orthogonal rendering of an IsoNet2-processed FIB-milled tomogram of C. reinhardtii" doi.org/10.64898/202... Since I am no expert, what does the cryoET community think of IsoNet? @cellarchlab.com doi.org/10.1038/s414...
- Reposted by Florian WollweberHoly cow the Zhou lab at UCLA is cooking with Isonet 2 - www.biorxiv.org/content/10.6... Averaging-free direct visualization of ribosome translational state! #teamtomo #cryoet
- Reposted by Florian WollweberVery excited to share that our project SPRING has been selected for funding in the ERC CoG 2025 grant competition. Many thanks to my fantastic team, colleagues @embl.org and all collaborators for their support.
- Here are some facts and figures from the 2025 Consolidator Grant call: 🔶 €728 million in funding 🔶 349 mid-career researchers from Europe and beyond 🔶 44 different nationalities All of them pushing the frontiers of knowledge. Congratulations! #ERCCoG #FrontierResearch #EUfunded #HorizonEurope
