Sven Klumpe
CryoFIB Development and In situ structural biology. NPC and TE enthusiast. Drosophilist but ready to switch to wherever biology takes us
Getting to enjoy science with a group at IMBA/IMP of the Vienna BioCenter imp.ac.at/groups/sven-klumpe
- Reposted by Sven KlumpeNew Article! Integrated fluorescence light microscopy-guided cryo-focused ion beam-milling for in situ montage cryo-ET
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- Reposted by Sven Klumpe#Zebrafish 🐟 meeting klaxon🧨 The registration period for the 1st German Zebrafish Meeting (16-18 Sept) is open www.germanfishmeeting.org with Sessions on Development, Pharma, Behavior, Omics, and more. Deadline for Abstracts at the end of April. Hope to see y'all there! 🧪 (1/n)
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- We are very excited to see this work out in its final form at Ultramicroscopy! Knife edge measurements of beam shapes and improved scanned versus shaped beam material removal comparisons. Find the OA article here: doi.org/10.1016/j.ul...
- Thrilled to share these experiments on shaping focused ion beams to explore different ways of milling. Here, we explore using different beam geometries to generate cellular thin sections/lamellae. The video shows elongating the beam on a charging spot burn www.biorxiv.org/content/10.1...
- Reposted by Sven KlumpeAre you an early-stage graduate student (2nd or 3rd year) or early-stage postdoc based in the US or Canada, working primarily in Drosophila? Would you like to help improve the experience of all trainees working in Drosophila research? If so, read on. (Please repost to reach a broad audience.)
- Join us at the Vienna BioCenter and @impvienna.bsky.social! Full disclaimer: I’m obviously biased but: mind-blowing facilities (!!!) and a great collaborative research culture make this an ideal place for anyone; especially suited to build up a lab. Plus Vienna is an amazing city to live in 😊
- 🌍Open call: Junior Group Leader positions! Join a world-class biomedical research institute at the heart of the Vienna BioCenter, where curiosity drives discovery. Lead your own lab, pursue bold ideas, and shape the future of science at the IMP: www.imp.ac.at/career/open-...
- Reposted by Sven KlumpeJust published! We share an open-source workflow to measure membrane thickness from tomograms, including a tutorial with 3D visualizations. We analyze thickness variations across organelles and reflect on where to define a membrane boundary. @becklab.bsky.social #teamtomo rupress.org/jcb/article/...
- Reposted by Sven KlumpeExcited to share our new @narjournal.bsky.social paper! Using ReLo and AlphaFold, we described a network of interactions between Drosophila piRNA pathway factors suggesting new links how they coordinate to ensure transposon silencing and genome integrity. academic.oup.com/nar/article/...
- Really awesome findings, congrats!
- 1/10 Genome maintenance by telomerase is a fundamental process in nearly all eukaryotes. But where does it come from? Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria. www.biorxiv.org/content/10.1...
- Congrats, Ulrich! Looking forward to see the amazing science your lab will do. Highly recommended for anyone searching for a PhD position!
- Thrilled to share that I’ll be joining @imbmainz.bsky.social in February 2026 to start my own group! We will explore new mechanisms in eukaryotic gene expression, leveraging ‘evolutionary play’ to uncover how regulation, repurposing, and hijacking shape RNA biology. PhD positions available!
- So good I’m almost worried it is fake but anyhow an amazing message to start the week to 😊
- Reposted by Sven Klumpe🎉Congrats to our @vdeneke.bsky.social, postdoc in the @pauligroup.bsky.social, who has received the 2025 Life Sciences Research Award Austria from the ÖGMBT! She's recognised for her groundbreaking work on the molecular mechanisms of fertilisation: www.imp.ac.at/news/article...
- Reposted by Sven KlumpeCongratulations to @vdeneke.bsky.social & Andreas Blaha @impvienna.bsky.social for their research on vertebrate fertilization, which was awarded this year's ÖGMBT Life Science Research Award in Austria for basic research!
- 🎉Congrats to our @vdeneke.bsky.social, postdoc in the @pauligroup.bsky.social, who has received the 2025 Life Sciences Research Award Austria from the ÖGMBT! She's recognised for her groundbreaking work on the molecular mechanisms of fertilisation: www.imp.ac.at/news/article...
- Reposted by Sven KlumpeInitial attempt at replicating in relion (parameters in next post). This is for Aca2-RNA, using a 100k subset of the 2D-classified particles (no prior 3D cleanup). A 1-class ab initio in relion, then local refinement in relion (1.8deg searches+blush) gives a nominally 3.3Å map; 3.5 Å w/out blush.
- Reposted by Sven KlumpeI am excited to share our new preprint on the CAGE complex, a mysterious hollow protein complex that I first saw years ago while surveying Tetrahymena ciliary lysate www.biorxiv.org/content/10.1... #cilia #protistsonsky 🧬🧪
- Reposted by Sven KlumpeThis one is a bit of a departure from the usual and definitely a work in progress! We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below. Read on for details... 1/x
- High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles biorxiv.org/content/10.1101/202…
- Reposted by Sven KlumpeBIG NEWS!! We will be hiring a new professor with 25 years of endowed funding at the @biozentrum.unibas.ch @unibas.ch, focused on using biophysics and structural biology to explore climate-related fundamental science topics. Come start an innovative research cluster with us! 🧪🔬🧶🧬🌾🌍
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- If you are thinking about the future of structural biology, we are too! Our two cents (Jürgen Plitzko and I) just got published online here: authors.elsevier.com/sd/article/S...
- So awesome this is out in its final form. Congrats @jennysachweh.bsky.social , @becklab.bsky.social and team. Happy I could contribute a little to the story 😊
- My PhD paper is in press @cellpress.bsky.social and now online: www.sciencedirect.com/science/arti...! Contents: weird #NPCs in yeast and flies, #CLEM, in situ #cryoET and more! Made possible by the amazing @becklab.bsky.social team at @mpibp.bsky.social and collaborators! #TeamTomo
- Extremely excited for the second version of this workshop at the MPI of Biophysics l, Frankfurt, that will happen 12-20. April 2026. Absolutely amazing lineup of speakers, I am very much looking forward to learning from everyone! Apply here: meetings.embo.org/event/26-in-...
- Reposted by Sven KlumpeFlyBase, a Drosophila database, will lose a third of its team in early October because the Harvard grant that covered the employees’ salaries was canceled. Scientists warn that losing FlyBase could devastate fly research. By @claudia-lopez.bsky.social www.thetransmitter.org/community/ha...
- Behind the paper article by Manel Llado Santaeularia on our manuscript on the in cell structure of a transposable element with @juliusbrennecke.bsky.social. Looking forward to exciting years @viennabiocenter.bsky.social Special thanks @sramundo.bsky.social for making this interaction possible!
- Reposted by Sven KlumpeExactly two weeks left to submit your abstract to the EMBO Mobile Genome Workshop. ☝️this year we will select from abstracts 2 “long” talks to highlight last minute breakthrough from early career scientists. Apply! July 29th
- ⏰ Abstract deadline for 'The mobile genome' is 29 July! 👉 s.embl.org/mge25-01-bl Join us 4–7 Nov 2025 at EMBL Heidelberg (or online) to explore the impact of TEs across biology. 🧬🔍 ⭐🧑🏼🔬 24 talks + 15 flash talks from posters – don't miss out! #EMBOMobileGenome
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- Reposted by Sven KlumpeWe’ve uncovered Asgard chromatin structures formed by a Hodarchaeal histone : closed hypernucleosome conserved in archaea and an open form resembling the H3-H4 eukaryotic octasome. Fantastic work by @harshranawat.bsky.social! www.biorxiv.org/content/10.1... #Chromatin #Asgard #Archaea #cryoEM
- Reposted by Sven KlumpeHey #TeamTomo, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why @florentwaltz.bsky.social and I made this website! tomoguide.github.io Follow the thread 1 /🧵 #CryoET #CryoEM 🔬🧪
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- Thrilled to share these experiments on shaping focused ion beams to explore different ways of milling. Here, we explore using different beam geometries to generate cellular thin sections/lamellae. The video shows elongating the beam on a charging spot burn www.biorxiv.org/content/10.1...
- Currently in FIB milling, a focused probe is used to scan an area that is supposed to be removed in order to create a thin section. However, the geometry of the thin section is very particular: ~8-20 µm wide and, in comparison to the width, extremely thin (~200 nm) Image from doi.org/10.1016/j.sb...
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- Reposted by Sven Klumpe🤯 cryo-ET of copia viral-like particles lurking at the nuclear pore of intact Drosophila eggs! Amazing work by @svenklumpe.bsky.social & more cool findings in this collaborative paper illuminating the replication cycle of an iconic retrotransposon! www.cell.com/cell/fulltex...
- Reposted by Sven KlumpeExcited to share our latest work, led by the incredible @automnenine.bsky.social (now an Ass. Prof. at Princeton)! We uncover key insights into the retrotransposition mechanisms of LINE-1 ORF2p, the enzyme responsible for writing a significant portion of our genome. www.science.org/doi/10.1126/...
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