Beck Laboratory
- Reposted by Beck LaboratoryIt’s out! Using cryo-ET in Dicty cells, we take a fresh in situ look at vaults. Surprisingly, we uncover vaults associated with ER and NE membranes, and find that many vaults enclose ribosomes in defined orientations, opening new avenues to their cellular function! www.biorxiv.org/lookup/conte...
- The vault associates with membranes in situ biorxiv.org/content/10.64898/20…
- Reposted by Beck Laboratory🎉 We’re absolutely thrilled: SCALE has been selected as a DFG Excellence Cluster! A huge thank you to our incredible team—this would not have been possible without your dedication and talent. 🙌 @dfg.de #ClustersOfExcellence #SCALEcluster 🧵
- (1/3) Together with the labs of Valerie Doye @vdoye.bsky.social and Hans-Georg-Kräusslich, we analyzed NPC structure in Nup133 null mESCs. We found heterogenous NPCs featuring non-canonical symmetries and missing subunits. Now online @naturecellbiology.bsky.social. www.nature.com/articles/s41...
- (2/3) Nup133 is a conserved component of the Y-complex, its deletion however is surprisingly is not lethal, but causes tissue and cell-type specific defects. We found that NPCs in Nup133-/- cells over-stretch and disintegrate upon induction of neuronal differentiation.
- (3/3) We propose a model, where the NPC dynamically acts to buffer tension applied to the nuclear envelope, thereby protecting it from potential rupture. Aberrations of the NPC scaffold would cause a reduced buffering capacity and result in NPC disintegration and potential rupture.
- Reposted by Beck LaboratoryExcited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social, @marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social 🔗 Preprint here tinyurl.com/3a74uanv
- Reposted by Beck LaboratoryNew preprint on 3D heterochromatin architecture in human cells! Great collab with @sergiocruzleon.bsky.social & @johannesbetz.bsky.social from @hummerlab.bsky.social, @marinalusic.bsky.social & the Turoňová lab. Many thanks to my supervisor @becklab.bsky.social. bioRxiv: tinyurl.com/3a74uanv 🧵👇
- In a great collaboration with @hummerlab.bsky.social and the Kräusslich lab: HIV capsid doesn't break at the NPC; instead, it cracks open the NPC itself! Details in Cell: authors.elsevier.com/sd/article/S... @mpibp.bsky.social @uniheidelberg.bsky.social A thread below:
- @jpkreysing.bsky.social, @maziarheidari.bsky.social, Vojtech Zila, and others studied HIV-1 capsid nuclear entry in primary human macrophages using STED, CLEM-ET, cryo-ET (#TeamTomo), and MD simulations. First, 3D STED revealed capsid signal accumulation at nuclear pores.
- The HIV capsids were detected on the cytoplasmic side, inside the central channel, and on the nuclear side of the NPCs. These data suggest that passage through the NPC is a significant hurdle for the capsid.
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View full threadSummarized, our data suggest a HIV-1 nuclear entry model where first a CypA coat around the capsid gets stripped away while the capsid is drawn into the FG-Nup mesh of NPC. Then the compression of the FG-Nups forces the NPC scaffold to crack allowing further progression of the capsid.
- Reposted by Beck LaboratoryFresh out of the press! ✨Check out our new paper on the violent entry of the HIV capsid into the nuclear pore — and how it cracks the gate! 🤯 💥 Incredible work from @becklab.bsky.social and @hummerlab.bsky.social 🤩 More here: www.biophys.mpg.de/2842024/hivcapsid-cracks-the-npc?c=2011019 🔗 #teamTOMO
