Jan Philipp Kreysing
in cell cryo-ET and image processing enthusiast #teamtomo
PhD student in Martin Beck's group (@becklab.bsky.social) at the Max Planck Institute of Biophysics (@mpibp.bsky.social)
- Reposted by Jan Philipp KreysingWe are excited to share our new preprint which is now available to read on biorXiv: doi.org/10.64898/202... 🎉🎉🎉
- #teamtomo strikes again! Check out our new preprint on the enigmatic vault and its newly discovered association with membranes in situ.
- It’s out! Using cryo-ET in Dicty cells, we take a fresh in situ look at vaults. Surprisingly, we uncover vaults associated with ER and NE membranes, and find that many vaults enclose ribosomes in defined orientations, opening new avenues to their cellular function! www.biorxiv.org/lookup/conte...
- Reposted by Jan Philipp KreysingNew paper out! How can we force HIV genomes out of their capsid shells and what can we learn about HIV biology doing so? Hint: capsid inhibitors actively re-route nuclear HIV complexes, and expose genomes via intriguing structural effects! open access: link.springer.com/article/10.1... read below
- Exciting new paper from my colleague @deglushk.bsky.social in the @becklab.bsky.social! It introduces a fast, robust tool for measuring membrane thickness in tomograms #TeamTomo.
- Just published! We share an open-source workflow to measure membrane thickness from tomograms, including a tutorial with 3D visualizations. We analyze thickness variations across organelles and reflect on where to define a membrane boundary. @becklab.bsky.social #teamtomo rupress.org/jcb/article/...
- Reposted by Jan Philipp KreysingHave you registered to the next EMBO Practical Course on In-situ structural biology by Cryo-FIB and Cryo-ET? 🧊 Join us in Frankfurt for hands-on training on cryo-techniques and networking with experts! 🔗 Apply by Dec 8, 2025: meetings.embo.org/event/26-in-... 📍 Frankfurt | 📆 April 12–20, 2026
- Reposted by Jan Philipp KreysingOur colleagues Vinith Kishore and Valentin Debarnot from the @ivandokmanic.bsky.social lab have come up with an amazing deep learning tool for denoising and filling the missing wedge in #cryoET data. I'm pleased to introduce Icecream🍧
- Reposted by Jan Philipp KreysingCheck out our preprint! With new molecular mechanisms, 140 subtomogram averages, and ~600 annotated cells under different conditions, we @embl.org were able to describe bacterial populations with in-cell #cryoET. And there’s a surprise at the end 🕵️ www.biorxiv.org/content/10.1... #teamtomo
- ❓What if you could peek between your tilt series ❓ We are proud to introduce cryoTIGER -- deep-learning based tilt interpolation for enhanced reconstruction in cryo-ET. A great collaboration between Turoňová lab, @becklab.bsky.social, @hummerlab.bsky.social #teamtomo www.nature.com/articles/s42...
- The software acts as a computational shortcut to denser sampling, which is often not feasible due to radiation damage limits. → Better angular sampling, sharper segmentation, improved particle localization.
- More great cryo-ET benchmarking work from my colleagues Maarten Tuijtel, Tomáš Majtner, Beata Turoňová, & @becklab.bsky.social: Systematic study of how tilt increment choice affects downstream TM & STA. Preprint has the optimally balanced tilt increment. #TeamTomo www.biorxiv.org/content/10.1...
- Reposted by Jan Philipp KreysingWe’re hosting the @embo.org Practical Course on In-situ structural biology by CryoFIB and CryoET in April 2026! 🧬❄️ You can expect hands-on training, cutting-edge methods, and a chance to work alongside leading experts in structural biology. 🔗 Apply by Dec 8, 2025: meetings.embo.org/event/26-in-...
- Reposted by Jan Philipp KreysingAI for Cryo-ET 🌟 Our new paper presents a deep-learning framework that cleans up faulty tilt series in a more automated, precise and powerful way! 💪 Read more here: www.sciencedirect.com/science/arti...
- New from my colleagues Markus Schreiber & Beata Turoňová: TANGO, an #opensource tool for more holistic spatial analysis of cryo-ET particles. It can clean or cluster particles, analyze lattices, and identify patterns. #TeamTomo www.biorxiv.org/content/10.1...
- Reposted by Jan Philipp KreysingHey #TeamTomo, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why @florentwaltz.bsky.social and I made this website! tomoguide.github.io Follow the thread 1 /🧵 #CryoET #CryoEM 🔬🧪
- Reposted by Jan Philipp Kreysing🦠🧠 MemBrain update! 🧠🦠 We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics. 🔗 Preprint: doi.org/10.1101/2024... 🔗 Code: github.com/CellArchLab/... 🧵(1/6) #TeamTomo
- Looking for a tool to measure membrane thicknesses in tomograms (#TeamTomo)? Or curious about thickness differences within and across organelles? Check out my fellow @becklab.bsky.social PhD student @deglushk.bsky.social's excellent preprint: www.biorxiv.org/content/10.1...
- New preprint on 3D heterochromatin architecture in human cells! Great collab with @sergiocruzleon.bsky.social & @johannesbetz.bsky.social from @hummerlab.bsky.social, @marinalusic.bsky.social & the Turoňová lab. Many thanks to my supervisor @becklab.bsky.social. bioRxiv: tinyurl.com/3a74uanv 🧵👇
- We investigated the molecular architecture of heterochromatin using cryo-ET (#TeamTomo) with enhanced template matching, subtomogram averaging, DNA linker predictions, and MD simulations.
- First, we enhanced template matching for nucleosomes by generating interpolated tilts between existing ones to reconstruct higher-contrast tomograms. Check out www.biorxiv.org/content/10.1... for method details. #TeamTomo
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View full threadFinally, we selected a representative fragment of chromatin, modelled it including histone tails, and performed all-atom MD simulations on it. The simulation showed an overall stable molecular arrangement with H4 tails mediating a stacking interaction.
- Also, check out @sergiocruzleon.bsky.social's post for a cool video summary about our new heterochromatin preprint.
- Excited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social, @marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social 🔗 Preprint here tinyurl.com/3a74uanv
- Excited to share our latest work on nuclear pore complex scaffold integrity and its perturbation. Now out in Nature Cell Biology: rdcu.be/eg4i9 Great work by Reiya Taniguchi who just opened his own research group back in Japan! 🥳
- Reposted by Jan Philipp KreysingAutomated Removal of Corrupted Tilts in Cryo-Electron Tomography biorxiv.org/content/10.1101/202…
- Reposted by Jan Philipp KreysingHello world!
- Reposted by Jan Philipp KreysingFresh out of the press! ✨Check out our new paper on the violent entry of the HIV capsid into the nuclear pore — and how it cracks the gate! 🤯 💥 Incredible work from @becklab.bsky.social and @hummerlab.bsky.social 🤩 More here: www.biophys.mpg.de/2842024/hivcapsid-cracks-the-npc?c=2011019 🔗 #teamTOMO
- So happy that my main PhD project on HIV-1 nuclear entry is now out in Cell. I want to especially thank my supervisor Martin @becklab.bsky.social and our amazing collaborators @maziarheidari.bsky.social from @hummerlab.bsky.social, Vojtech Zila from the Kräusslich lab and many others.
- In a great collaboration with @hummerlab.bsky.social and the Kräusslich lab: HIV capsid doesn't break at the NPC; instead, it cracks open the NPC itself! Details in Cell: authors.elsevier.com/sd/article/S... @mpibp.bsky.social @uniheidelberg.bsky.social A thread below:
- Reposted by Jan Philipp KreysingcryoTIGER: Deep-Learning Based Tilt Interpolation Generator for Enhanced Reconstruction in Cryo Electron Tomography biorxiv.org/content/10.1101/202…
- Exciting times in HIV research and prevention! HIV capsid is a great drug target because it's not just a protective shell for the viral genome but also a key facilitator for HIV entering the nucleus via the nuclear pore (NPC).
- Science’s 2024 Breakthrough of the Year is lenacapavir, an injectable drug that demonstrated remarkable success at preventing HIV infection with one shot every 6 months. Learn more about this year's #BOTY and other big advances in science: scim.ag/3BrCtUn
- Check out our latest preprint if you want to find out what happens when capsid and NPC interact (spoiler: it doesn't seem to end well for the NPC) #teamtomo www.biorxiv.org/content/10.1...
- Reposted by Jan Philipp KreysingCelebrating Dr. Huaipeng Xing from #molecularsociology on successfully defending his #PhD! 👏🥳 He studied the visualization of protein synthesis and folding inside human cells by #cryoET #teamTOMO. Congrats, Huaipeng, and best wishes for your future! 🤩
- Reposted by Jan Philipp KreysingDo not miss our latest #cryoET work! 🕵️ Xing et al. present the dynamic architecture of TRiC in human cells! The study of this protein-folding chaperonin opens the door to understanding its dysfunction in diseases like cancer and neurodegeneration 🧠🧪 #teamTOMO @becklab.bsky.social
- Reposted by Jan Philipp KreysingAre you already on track to apply to our #gradschool? 👣 This is a little map of our application process! We hope, you don´t get lost on the way 😊 If you want to start your #phdjourney with IMPRS-CBP #applynow Don’t miss the chance and apply by February 7th. 👉 imprs-cbp.mpg.de 👈 #imprs_cbp
- Reposted by Jan Philipp KreysingHello 🦋! We’re excited to announce that applications for our #PhDprogram open in TWO weeks! 🤯 Join our #IMPRS and take your first steps toward groundbreaking research in Cellular Biophysics. 📅 Mark your calendar & stay tuned: imprs-cbp.mpg.de #PhDPositions #GradSchool #MaxPlanckSociety
- Reposted by Jan Philipp KreysingFaster and more accurate protein modeling with CryFold. By focusing on nearby points (Local Attention) and employing map masking, CryFold excels in generating models from challenging maps regions. It enables a more cost-efficient model building. 🧵 💾 github.com/SBQ-1999/Cry...
