Dimitrios L. Wagner
Physician & science enthusiast @BCMHouston @ChariteBerlin @BIHregeneration // Genome Editing and Immunology // 50% of @immunoboys.bsky.social / @dlwagnerlabs.bsky.social
- Reposted by Dimitrios L. WagnerRotation PhD student @emiliedevet.bsky.social in our lab made the Dish soap protocol work (on the first attempt) for a nuclear target with Dawn (the US version of dreft/fairy ) and thereby extended the dish soap protocol to a version for across the atlantic 🤩 @tothovalab.bsky.social #hemesky
- For our #flowcytometry peeps, would you like to have a single fix/perm protocol that is optimised for everything? One that preserves fluorophores while allowing simultaneous TF and cytokine staining? How about 100-fold cheaper? You got it: currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...
- Reposted by Dimitrios L. WagnerDistinct EBV-Associated Phenotypes Due to a Novel Homozygous Missense Variant in CD27. biorxiv.org/content/10.64898/20…
- Reposted by Dimitrios L. WagnerTogether with @ronghuizhu.bsky.social, we are thrilled to present our new perturb-seq study of 22M primary CD4+ T cells, across donors and timepoints – the result of a decade-long collaboration between the Marson @marsonlab.bsky.social and Pritchard @jkpritch.bsky.social labs 🧵 tinyurl.com/gwt2025
- Reposted by Dimitrios L. WagnerTerrific to have our paper, led by @tjflemin.bsky.social, featured as a plenary article in @bloodjournal.bsky.social today: ashpublications.org/blood/articl...
- Reposted by Dimitrios L. Wagner🚨This week in @natcomms.nature.com: We resolve an enduring enigma in T cell biology—how TCR binding to p/HLA triggers intracellular signaling. CryoEM in native environment reveals a 'jack-in-the-box' mechanism! Collab btw @rockefeller.edu @mskcancercenter.bsky.social rdcu.be/eUPPl
- Reposted by Dimitrios L. WagnerClinical development of gene edited tacrolimus-resistant Treg (FKBP12KO-Treg) to enable simultaneous immunosuppression and support of immune regulation biorxiv.org/content/10.64898/20…
- Reposted by Dimitrios L. WagnerOriginal work here ⬇️, led by the inimitable Lorenzo Brunetti and Michael Gundry. This work became foundational in the field.
- Reposted by Dimitrios L. WagnerToday's the print release of a #RibackLab and #GoodellLab collaboration— we’re excited to share the visual story behind the science, including the dramatic artwork that inspired how we thought about aberrant function of NPM1c - which occurs in about 1 in 3 adult acute myeloid leukemias (#AML).
- Reposted by Dimitrios L. WagnerA big advance, folks, for off-the-shelf, universal, engineered (base-edited) T cells achieving complete response vs incurable leukemia in several patients www.nejm.org/doi/full/10....
- Reposted by Dimitrios L. WagnerOur case report of a patient developing drug-induced systemic sclerosis after bleomycin exposure – still showing antibodies after 5 years. Nicely done Philipp 🎉 #rheumsky authors.elsevier.com/sd/article/S...
- Reposted by Dimitrios L. WagnerExtrusive and cohesive cohesin cooperate to repair double-strand breaks in DNA @science.org @albertomarin.bsky.social @gerlichlab.bsky.social @imbavienna.bsky.social www.science.org/doi/10.1126/... www.science.org/doi/10.1126/... www.science.org/doi/10.1126/...
- Reposted by Dimitrios L. WagnerBig news! Our team developed Neuro-PERC, a non-viral CRISPR delivery platform for genome editing of the brain. In our new preprint - bit.ly/NeuroPERC_pre - we report efficient editing in multiple models & species, including field-first results in large animals (pigs) 🧵👇
- Reposted by Dimitrios L. WagnerOn this #WorldAIDSday @cgaebler.bsky.social, Olaf Penack & teams at #CharitéBerlin report the #SecondBerlinPatient cured of #HIV after heterozygous CCR5Δ32 HSCT. The study opens exciting perspectives for immune-based #HIVCure strategies for longterm ART-free viral control and ultimately cure.
- Reposted by Dimitrios L. Wagner[This post could not be retrieved]
- Reposted by Dimitrios L. WagnerAdapting the 10x workflow to detect EBV reads, Younis et al show that EBV-transcribing B cells are increased in SLE patients, especially among CD27+CD21low B cells. They have autoreactive BCRs and are more poised for antigen-presentation. 2025 Sci Transl Med 🧪 ⑂ www.science.org/doi/10.1126/...
- Reposted by Dimitrios L. WagnerWhat mechanisms drive checkpoint inhibitor-associated interstitial nephritis (ICI-AIN)? In our preprint, we identify CD8 T cells as a central player, inducing a proinflammatory myeloid cell state by IFNg and structuring lymphoid aggregates by CXCL13. www.biorxiv.org/content/10.1... 1/n #Nephsky 🧪
- Reposted by Dimitrios L. WagnerA monoclonal antibody that inhibits the shedding of CD16a and CD16b and promotes antibody-dependent cellular cytotoxicity against tumors @natcomms.nature.com www.nature.com/articles/s41...
- Reposted by Dimitrios L. Wagner(1/10) How do diverse leukemia mutations converge on the same molecular program? In #RibackLab first manuscript @cp-cell.bsky.social, collaboration with @goodell-lab.bsky.social shows that disparate mutations rewire shared protein networks to form nuclear condensates called C-bodies.
- Reposted by Dimitrios L. WagnerUbiquitinylation is more complex than just a signal for proteasomal degradation: Linear ubiquitinylation of Methionine-1 is important for NFkB signalling. LOF of the Deubiquitinylase OTULIN leads to an autoinflammatory disease with a TNF positive feedback loop. 🧪 ⑂ 1/2
- Reposted by Dimitrios L. WagnerEver noticed that #CRISPR editing results differ between cells? Awesome PhD student Moritz Schlapansky developed "scOUT-seq" to measure single cell transcriptomes + editing. 1.2 million cells, 74 cell types, living 🐭. Cell subtypes differ wildly from bulk average! www.biorxiv.org/content/10.1...
- Reposted by Dimitrios L. WagnerA new subspecialty takes off! Astroimmunology is focused on the effects of #spaceflight on the #immunesystem and defines avenues for countermeasures. A bonus: the work will also inform research on #aging @natrevimmunol.nature.com evimmunol.nature.com www.buckinstitute.org/news/a-hitch...
- Reposted by Dimitrios L. WagnerBIG ANNOUNCEMENT📣: I haven’t been this excited to be part of something new in 15 years… Thrilled to reveal the passion project I’ve been working on for the past year and a half!🙀🥳 (thread 👇)
- Reposted by Dimitrios L. WagnerWhat is a promoter? And how does it work? We very happy to share our latest work trying to understand enhancer-promoter compatibility. I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters www.biorxiv.org/content/10.1...
- Reposted by Dimitrios L. WagnerHonored to be part of the 2025 #STATWunderkinds! Grateful for the many supportive mentors, colleagues, and friends with special shoutout to @bkleinstiver.bsky.social. Full list: www.statnews.com/wunderkinds/ @statnews.com @mitdeptofbe.bsky.social @mgbresearch.bsky.social @harvardmed.bsky.social
- “ ‘Off the shelf’ Treg may soon be… on the shelf. “ - awesome quote by Prof. Fadi Issa. Check out our new story: 👇 www.nature.com/articles/s41... One week after the Nobel prize for Treg 🍀
- “Good things take time” Allogeneic Treg do not prevent their own rejection in immunocompetent hosts - HLA matching or HLA editing required for optional function! A beautiful collaboration with Fadi Issa & Joanna Hester’s team @ox.ac.uk. @dlwagnerlabs.bsky.social
- HLA matching or CRISPR editing HLA-I/II enables engraftment & function of allo human Treg therapy in a humanized mouse transplantation model @natcomms.nature.com @dlwagner.bsky.social @bcmhouston.bsky.social www.nature.com/articles/s41...
- Reposted by Dimitrios L. WagnerHLA matching or CRISPR editing HLA-I/II enables engraftment & function of allo human Treg therapy in a humanized mouse transplantation model @natcomms.nature.com @dlwagner.bsky.social @bcmhouston.bsky.social www.nature.com/articles/s41...
- @dlwagnerlabs.bsky.social on tour! 🌍🇩🇪🇺🇸 We had a great time at the Annual Congress of the European Society for Gene and Cell Therapy #ESGCT2025 in Sevilla. 🇪🇸 Super proud of everyone who presented their projects & grateful for working with this amazing team: science and good vibes! 🧬🧪💙
- Reposted by Dimitrios L. Wagner
- Reposted by Dimitrios L. WagnerTCR activation impairs CAR-T cytotoxicity against separate target cells biorxiv.org/content/10.1101/202…
- Reposted by Dimitrios L. WagnerOur team is on their way to the ESGCT conference 2025! Excited for this upcoming week full of science, talks, and inspiring discussions with the cell & gene therapy community! Starting with the HDR workshop - talks by Isabell (‘one-pot’ PASTA) & Viktor (BEKI) + posters by Ana, Lily, Jonas and Laura
- Reposted by Dimitrios L. WagnerExcited to share our preprint📯: We introduce #IntegrateALL, an open-source RNA-seq pipeline for interpretable molecular diagnostics in acute lymphoblastic leukemia!🧬 Big thanks to Nadine Wolgast & team @ www.uksh.de , catchall-kfo5010.com! See: doi.org/p7k5 and 🧵
- Reposted by Dimitrios L. WagnerBig shoutout to the whole group who made #IntegrateALL possible - especially @alinamh.bsky.social, Thomas Beder and Claudia Baldus at www.catchall-kfo5010.com
- Reposted by Dimitrios L. WagnerPotentiating CAR-T bystander killing by enhanced Fas/FasL signaling mitigates antigen escape in heterogeneous tumors biorxiv.org/content/10.1101/202…
- Reposted by Dimitrios L. WagnerCheck out one of our latest stories about combining our non viral knock-in strategy with integrases for an efficient, targeted integration of transgenes >8kb in human T cells! ‘One-pot’ PASTA: programmable and site specific transgene addition - all in just one reaction 🫕
- You like engineering T cells, but you are missing the right flavor? Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1 We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt 𝐏rogrammable 𝐀nd 𝐒ite-specific 𝐓ransgene 𝐀ddition 🧪
- Reposted by Dimitrios L. WagnerThis has been such a great collaboration with @dlwagnerlabs.bsky.social! You really need to check it out One-pot PASTA 🍝 - a one-step protocol to knock-in large transgenes. I feel tempted to think about one-pot PIZZA 🍕 next! First authors Isabell and @therealjonaskath.bsky.social were amazing!!
- You like engineering T cells, but you are missing the right flavor? Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1 We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt 𝐏rogrammable 𝐀nd 𝐒ite-specific 𝐓ransgene 𝐀ddition 🧪
- Reposted by Dimitrios L. WagnerSuper-large targeted Knock-Ins in primary T cells, with multigene inserts >8kb, in a single reaction. HOW?? 🙀 Combine simple CRISPR-HDR & Integrases for one-pot PASTA🍝 = Programmable And Site-specific Transgene Addition. 😎
- You like engineering T cells, but you are missing the right flavor? Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1 We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt 𝐏rogrammable 𝐀nd 𝐒ite-specific 𝐓ransgene 𝐀ddition 🧪
- You like engineering T cells, but you are missing the right flavor? Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1 We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt 𝐏rogrammable 𝐀nd 𝐒ite-specific 𝐓ransgene 𝐀ddition 🧪
- 'one-pot' PASSTA builds on 4 simple reagents that are easy to source & GMP compatible! 1. CRISPR-cas nuclease 2. HDR template (for landing pad insertion) 3. serine integrase mRNA 4. plasmid with cargo -> a single electroporation in proliferating T cells = ✨ Efficient integration of XL-genes ✨
- Super versatile; exchanging just nuclease + template enables you to put your cargo in a different place. 📍🗺️
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View full threadLed by the integrase lover Isabell Kassing (lawyer turned biologist) and our one and only @therealjonaskath.bsky.social. Amazing collab with @danielibrahim.bsky.social who pushed this idea. Lots of help from the @dlwagnerlabs.bsky.social and Alessa Ringel! ❤️
- 🚀 In preparation for releasing two new stories, @therealjonaskath.bsky.social is revisiting the journey so far—sharing the lab’s last six papers. What a ride it’s been… 🙌🧬🧪🧫
- Reposted by Dimitrios L. Wagner🚨 Paper alert! "Next-gen multiplex-edited CAR-NK cells: more edits, more power?" @jitc @bmj.com With @dlwagner.bsky.social we discuss Wang et al.’s landmark paper on multiplex base-edited non-viral CAR-NK cells. Promising efficiency but safety challenges! jitc.bmj.com/content/13/9...
- Reposted by Dimitrios L. WagnerPlasmacytoid dendritic cells can transition to become cDC2s under to influence of TNF, while type-I interferon reduces TNFR expression and thereby inhibits transition. These transitioned cDC2 seem fully functional. 🧪 ⑂ Hornero et al 2025 in Nat Immunol www.nature.com/articles/s41...
- Reposted by Dimitrios L. Wagner23 tools to work with (single-cell) TCR/BCR-seq immune repertoire data 🧵 👇
- Reposted by Dimitrios L. WagnerDoomscrolling pause to chat CRISPR libraries! Preprint describes our new, data-driven approach to combine on-target and off-target predictions much more intelligently for *selecting* guides, which we use to develop our newest Cas9 knockout library, Jacquere. Thread: www.biorxiv.org/content/10.1...
- Reposted by Dimitrios L. WagnerHorisberger et al separate Lupus nephritis patients in 3 groups by blood: healthy-like, interferon-dominant and CD8 T cell-dominant. The CD8 T cell-dominant group had the highest biopsy activity score and highest response rate after 1 year; the healthy-like the most chronic damage. 🧪 ⑂ #NephSky 1/
- Reposted by Dimitrios L. WagnerA synonymous mitochondrial variant is negatively selected against in short-lived CD8 effectors – How? The variant changes tRNA pairing from Watson-Crick-Franklin to wobble, thereby reducing translational efficiency in high energy demand. 🧪 ⑂ Lareau et al – 2025 www.pnas.org/doi/10.1073/...
- Reposted by Dimitrios L. Wagner🚨 First-ever FOCIS Journal Club! 🎓 Blood Immunophenotyping Identifies Distinct Kidney Histopathology and Outcomes in Patients with Lupus Nephritis 🗓️ Oct 13 | 🕚 11 AM CT 🎤 Dr. Rao & Dr. Horisberger 💥 FREE for all! (Non-members need code)
- So accurate, it hurts
- Reposted by Dimitrios L. WagnerJournals in August
- Reposted by Dimitrios L. WagnerIn Lupus nephritis patients, neutrophils have increased surface LAMP1, which is externalized after activation. Soluble LAMP1 is increased both in the blood and urine of patients, correlating with histological activity. #NephSky 🧪 Our paper in @eurjimmunol.bsky.social doi.org/10.1002/eji....
- Reposted by Dimitrios L. Wagner
- Reposted by Dimitrios L. WagnerQ4ddPCR (May the Fourth Be Precise): A Flexible, 4-Target Assay for High-Resolution HIV Reservoir Profiling biorxiv.org/content/10.1101/202…
- Reposted by Dimitrios L. WagnerMy colleagues at Nature Biomedical Engineering @natbiomedeng.nature.com are hiring an associate or senior editor. Ideal applicant will have expertise in immunology, immunotherapy, or immuno-oncology. Please feel to ask me questions! springernature.wd3.myworkdayjobs.com/SpringerNatu...
- Reposted by Dimitrios L. WagnerResting MAIT cells generally express the non-cytotoxic Granzyme K, but rapidly switch to the cytotoxic Granzyme B upon stimulation. Consequently, previously stimulated MAITs kill much more target cells than resting ones. 🧪 Kurioka et al – 2015 – @mucosalimmunol.bsky.social doi.org/10.1038/mi.2...
- Reposted by Dimitrios L. WagnerIt's my first #newPI roll call on BlueSky! Calling all new #cancer PIs who just started or who are about to start their labs! Please say hi and what you work on so I can follow you if I don't already! 😃
- Reposted by Dimitrios L. WagnerBye forever, WeTransfer.
- Reposted by Dimitrios L. WagnerOxidized mitochondrial DNA induces NLRP3 activation in pDCs. This leads to enhanced pDC function beyond IFNa production so that they produce IL1b, IL6 and IL21, inducing Tfh differentiation and autoimmunity (in 🐭). Xian et al – 2025 @natureportfolio.nature.com www.nature.com/articles/s41... 🧪⑂
