Jaroslav Icha
Light microscopy at Bruker Fluorescence Microscopy division.
#lightsheet #multiphoton #superresolution #screening
Cell biologist in my previous life. Alumnus @mpicbg.bsky.social
@turkubioscience.bsky.social
- Deadline for abstract submission in one week! I'm sharing this reminder because right after the conference we have an open demo day at DZNE Bonn, which will include voltage imaging on Bruker 2P microscope and Ca imaging in freely moving mice with Inscopix miniscopes www.bruker.com/en/news-and-...
- We are hosting a demo day for @brukercorporation.bsky.social two photon microscopes and Inscopix miniscopes. 📅 March 26th 2026 📌 DZNE in Bonn Whole day event with hands on sessions, presentations and discussions with our existing 2P users. Register: www.bruker.com/en/news-and-...
- We (Bruker Fluorescence Microscopy) will run a satellite event on March 26th. A mixture of a hands on demo of our 2P and miniscopes and our user meeting. One can attend independently of the conference, so this is a free event for anyone who can make a daytrip to Bonn. www.bruker.com/en/news-and-...
- 💡 Join the 8th Bonn Brain Conference March 23–25, 2026 | DZNE Bonn, Germany Explore the latest in brain states, neural circuits, and behavior — with keynote talks by leading neuroscientists including Cori Bargmann and Edvard Moser @edvardmoser.bsky.social
- First @brukercorporation.bsky.social (and mine) lightsheet microscope sale in Poland and it is straight away our most complex lattice system even with an IR laser photomanipulation module. Joint project with our distributor in Poland Labsoft.
- The Luxendo InVi Lattice Pro is a good lattice system for imaging facilities like the one at the Nencki Institute because of its versatile sample mounting strategies and flexibility in configuration - lasers, cameras.
- I expect exciting results from this microscopein the next few years. The momentum in polish science is building up and they are entering the first world league (sadly, unlike the other V4 countries Czechia, Slovakia, Hungary, where I rather see stagnation; politics play a role).
- I meant to post it long time ago but things have been busy. Still, this article is worth sharing even if not breaking news. Researchers at Tsinghua University developed a new approach to tissue clearing, VIVIT, using among other instruments, the Bruker MuVi SPIM www.sciencedirect.com/science/arti...
- Are you interested in long time lapse imaging of organoids? In this example, retinal organoids were imaged in high throughput for 3 days. The aim was to collect sufficient data to be able to use machine learning for predicting organoid differentiation just from brightfield images.
- Done on the Bruker FM Acquifer IM microscope: www.bruker.com/en/products-... preprint: www.biorxiv.org/content/10.1...
- For those at Seeing is Believing in Heidelberg right now, do join our workshop, which is about to start soon at 11:30! www.embl.org/about/info/c... Focus on organoid time lapse imaging, with high throughput options as well. #EESImaging
- We have just installed a third HIVE server in Czechia. After Prague and Brno, we are adding Olomouc! www.bruker.com/de/products-... Researchers often get HIVE to handle data from Luxendo as well as other companies' light-sheet scopes but it is a rather universal solution for big data management.
- Happy to be at the #V4SDB 2025 developmental biology conference. Amazing quality of the talks and a beautiful venue in the High Tatras in Slovakia. @v4sdb.bsky.social v4sdb2025.img.cas.cz/programme/
- Really nice thread about the infrared co-illumination to reduce photobleaching of GFP. I was aware of the Ludvíkova et al paper but didn’t realize that lightsheet is the main modality, which would benefit from it.
- We have just installed the high throughput microscope Acquifer IM from Bruker in the lab of Prof. Buchtova in Brno, Inst. of Animal Physiology and Genetics. This microscope is a versatile widefield fluorescence microscope, which is suited for imaging larger samples like organoids or fish embryos.
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View full threadThis installation is special for me because: - it is actually my first microscope sale since my role and territory changed within Bruker/Luxendo - it is in my home country – Czechia - it will be frequently used for zebrafish applications, which is close to my heart (I did 5 years of this in my PhD)
- If you are located in Brno and the surroundings and you would like to increase the throughput and automate your imaging experiments, I’m sure you can get in touch with Prof. Buchtova to give it a try.
- The IM expert in our team @jgehrig.bsky.social did most of the heavy lifting in microscope set up and training how to use it. I contributed rather with the literal heavy lifting :-) more about the device: www.bruker.com/en/products-...
- Post no. 2 on lightsheet microscopy in neuroscience. Imaging whole cleared brains This is probably the most obvious use of lightsheet but I want to highlight something specific, read on. First, why is lightsheet uniquely suited for imaging whole cleared brains?
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View full threadThe movie: Dumas et al., 2024, Research Square (preprint) www.researchsquare.com/article/rs-4... Whole mouse brain in situ hybridization to detect Pitx2 mRNA (a homeodomain transcription factor, magenta) autofluorescence (gray), DISCO-based clearing, 4x detection
- Imaging done on Bruker Luxendo LCS SPIM. www.bruker.com/en/products-... There is a range of lightsheets for cleared tissue imaging. One special feature of LCS is that it is amenable to high throughput and some of our users do exactly that. You need to image 100s of mouse brains? No problem!
- The whole brain in situ hybridization protocol to detect RNAs expressed in different regions of the brain was optimized in the laboratory of Per Uhlen lab at Karolinska Institute in Stockholm. www.science.org/doi/10.1126/...
- One very useful application is to detect transcripts of immediate early genes. There is a wide range of stimuli that triggers expression of IEGs in neurons. Their expression is a good proxy to record which neurons were affected/activated by a stimulus, irrespective of the nature of the stimulus.
- - creative mounting strategies tailored for whole cleared tissues. Important for fragile, expanded samples. - isotropic resolution in xyz can be achieved (multiview acquisition) - data handling is established (tiled acquisition setup, stitching, registration, fusion of stacks, visualization)
- The specific thing I want to highlight is that in the cleared brains one can detect not only proteins (stained with antibodies or tagged by a fluorescent protein) but also RNAs through in situ hybridization.
- - speed of acquisition. This matters when we talk about imaging centimeters3 of tissue. - low photobleaching of fluorescent labels during acquisition (only one plane is illuminated) - tissue stays intact. Thanks to long working distance objectives, you can image through a big piece of tissue.
- Looking forward to the conference in Wroclaw! I'll be there with our distributor Labsoft to talk mainly about Bruker's multiphoton and lightsheet microscopes and their applications in neuroscience.
- 📣 The #PTBUN invites the neuroscience community to the 17th International Congress of the Polish Neuroscience Society 🧠 📍Wroclaw, Poland | 🗓️ Sept 2–5, 2025 Join them for plenary lectures, symposia, and interdisciplinary exchange in a city of rich scientific heritage. #Neuroscience #Wroclaw2025
- If you want to run a (drug) screen on something bigger than just 2D cells - like zebrafish embryos or organoids, we have a perfect instrument for you, the Acquifer IM. My colleagues summarized this into a new app note. Take a look: www.bruker.com/en/products-...
- I’ve decided to do a few posts throughout the summer about using lightsheet microscopy in neuroscience, as part of my own learning. Today: calcium imaging Lightsheet with its widefield detection can be really fast and gentle to the sample, enabling 100s of frames per second imaging.
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View full threadvideo: (Walsh et al., Cell Reports, 2024) Ca2+ activity (GCaMP6m) in LIF treated human cerebral organoids 2 min time-lapse shown at 10x speed acquired at 10 fps www.cell.com/cell-reports... Imaged on Bruker Fluorescence Microscopy Luxendo TruLive 3D microscope www.bruker.com/de/products-...
- I think lightsheet could perform particularly well in experiments combining long term developmental time-lapse of organoids interleaved with short calcium imaging experiments. It is hard to imagine any other microscope that could do it.
- There, the authors could image the entire brain volume every 1.3 s for an hour with cellular resolution thanks to the speed and low phototoxicity of the lightsheet.
- An example I want to highlight here is from a publication that improved cerebral organoid cultivation and included some functional imaging – it makes sense to ask how “good” the generated neurons are in terms of amplitude and frequency of Ca2+ spiking.
- You won’t get as deep into your tissue of interest as with two-photon microscopy but many samples are rather transparent or you can measure close to the surface. Prime example being the zebrafish larvae, the iconic Ahrens&Keller publication from 2013 comes to mind www.nature.com/articles/nme...
- A great study showing the power of the zebrafish model. Methodologically rich from RNAseq, CRISPR screen to live and expanded sample imaging. It shows that nowadays you don't have to only image what is happening in development but you can also get to the mechanistic details, all using zebrafish.
- Interested in cell migration yet bored of the canonical translocation modes? 😴 Don’t worry — we @nordenlab.bsky.social with @aiopticalbiolab.bsky.social have multipolar migration back in stock! No need to follow the other cells: extend long protrusions and roam more freely🪼 in the crowded brain.
- Photomanipulation. Something available on most Bruker Fluorescence Microscopy microscopes. Multiphotons, Lightsheets, high throughput systems. Over the years we specialized in this and accumulated a lot of practical know-how.
- "Photomanipulation" can take the form of optogenetic stimulation of a whole neuronal circuit simultaneously in a living mouse, cutting actin cortex in a Drosophila embryo with pulsed femtosecond laser, localized wound in a fish fin with a 405 nm laser to trigger immune cel migration and a lot more.
- We have a new tech note about PM in high throughput on the Acquifer IM microscope. Give it a read if relevant for your research. I can guarantee that it works smoothly because that's exactly what we did a few weeks ago in a Polish Zebrafish Society workshop in Olsztyn. www.bruker.com/en/products-...
- Looking forward!
- 📢Only 21 days remain to register for THE #developmentalbiology #conference in Europe, the V4SDB Meeting 2025, that will take place on the 5th-7th September 2025 in 🇸🇰⛰️! Join us: v4sdb2025.img.cas.cz/registration... #morphogenesis #stemcells #evodevo #organoids #modeling #embryogenesis
- If you are interested in FCS, FLIM and related methods: Bruker Luxendo lightsheet microscopes can be equipped with these modalities. It is admittedly rather niche, but very powerful. We already have a lot of experience implementing it. Customer webinar about it⬇️ info.bns.bruker.com/2025-07-08-W...
- Corentin Rousset now introducing Bruker's Vutara VXL microscope to the EMBO course in superresolution microscopy in Prague. Practicals to follow later today with the generous help of Dr. Christoph Spahn. We will try z-stack acquisitions, STORM PAINT and PALM on bacteria. Looking forward!
- An opportunity to test @brukercorporation.bsky.social SMLM microscope Vutara VXL in Prague on 18th and 19th June before our practical at the @embo.org superres microscopy course @imgprague.bsky.social meetings.embo.org/event/25-sup... Let me know if interested.
- Great course that I hope to participate at a bit. @brukercorporation.bsky.social equipment from the organizing institutions will be involved: multiphoton, MRI
- It is really cool to see that someone is building upon an auxin-inducible protein degradation system designed in the Mansfeld lab that we optimized for zebrafish in @nordenlab.bsky.social (Daniel et al 2018) and the Acquifer IM microscope (something I sell these days) used for imaging as a bonus!
- Congatulations on a nice discovery to the Galjart lab! The long term high-resolution imaging of stem cells was done on our InVi Lattice Pro, a lattice lightsheet microscope from Luxendo @brukercorporation.bsky.social Lightsheet with low phototoxicity is the only modality allowing such experiments.
- If you want to learn about the microscope: It is built on our inverted platform InVi SPIM with an advanced illumination module delivering very flexible Gaussian, Bessel, lattice illumination. www.bruker.com/en/products-...
- New from Wittbrodt, Birney labs on genetics of heart rate&development. www.nature.com/articles/s41... This involved imaging heartbeat of A LOT of fish. Possible thanks to pipeline including the high throughput microscope Acquifer IM that I'm fortunate to sell ;-) www.bruker.com/en/products-...
- The YMIA would like to invite everyone interested in microscopy and image analysis to our weekly watch-parties. This Tuesday we cover how to choose the right microscope for your experiment as well as learning how to minimize damage to samples during light microscopy.
- Watchparty is the best rebranding of a „webinar“ i have seen so far :-D Jokes aside, it looks like a great event!
- Amsterdam - Utrecht - Rotterdam - Eindhoven - Bochum - Nijmegen - Leuven I made quite a roadtrip this week. Visiting @brukercorporation.bsky.social Fluorescence Microscopy / Luxendo existing microscope users and future ones as well. Exhausting but fun and learning a lot along the way.
- Multiphoton, lightsheet, superresolution, high throughput imaging, data management. I covered all of them this week. Can't really complain that the job would be monotonous. :-)
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- Registration is still open until April 14th: meetings.embo.org/event/25-sup...
