Jaroslav Icha: It is really cool to see that someone is building upon an auxin-inducible protein degradation system designed in the Mansfeld lab that we optimized for zebrafish in @nordenlab.bsky.social (Daniel et al 2018) and the Acquifer IM microscope (something I sell these days) used for imaging as a bonus!

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Jaroslav Icha handle.invalid
It is really cool to see that someone is building upon an auxin-inducible protein degradation system designed in the Mansfeld lab that we optimized for zebrafish in @nordenlab.bsky.social (Daniel et al 2018) and the Acquifer IM microscope (something I sell these days) used for imaging as a bonus!
- thomthum thomthum.bsky.social · May 26, 2025
  new #preprint: mRNA delivered degron system w/ GFP-nanobody to efficiently reduce GFP-tagged proteins in an inducible manner in 🐟 Case study: reduction of PMM2 (basis for ER-based #glycosylation) causes hypoglycosylation reported from CDG-patients www.biorxiv.org/content/10.1... #CRISPR #RareDisease
  Establishing an auxin-inducible GFP nanobody-based acute protein knockdown system to mimic hypomorphic mutations during early medaka embryogenesis
  
  Creating hypomorphic mutations are crucial to study gene function in vivo, especially when null mutations result in (embryonic) lethality. This is especially the case for enzymes involved in glycosyla...
  
  biorxiv.org
May 26, 2025 09:03
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