Richard Held
Neuroscientist / synaptic physiologist interested in protein topography and cellular signal transduction. Ephys in the past, cryoET now, mouse genetics forever.
scholar.google.com/citations?hl=en&use…
- Set my deadlift PR for the year 🤫
- Holiday triptych
- June does christmas right
- Holds up: www.mcsweeneys.net/articles/the...
- Absolutely insane paragraph
- Reposted by Richard HeldThe Nobel Prize committee should announce the World Cup winner tomorrow
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- I wrote a bit about practical considerations for using #cryo-ET labels and some things to work on if we want to find any protein in any cell. authors.elsevier.com/a/1mA6h,LqAr...
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- Not bad, not great
- So it begins
- Clearly a poorly resolved flexible domain here
- Finally got to go through this carefully. An interesting paper and a tremendous amount of work. www.science.org/doi/10.1126/...
- I'm not entirely on board with the proposed temporal sequence but I think the size of the dataset convincingly demonstrates the presence of "small" and "large" membrane proximal vesicle populations. A more tempting model imo would be that these small vesicles preferentially fuse asynchronously.
- Gonna need somebody to explain daylight savings to my dogs
- I think about this tweet on a near-daily basis
- This is what made America great
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- Heart rate data or RC voltage step?
- Saw someone designing a cloning strategy using chatgpt and for some reason that's kinda my breaking point in this particular discussion. How much thinking really needs to be outsourced for the sake of efficiency and who does that really serve in the end?
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- Reposted by Richard Held"The money the government sends to Harvard is, in effect, not a subsidy to advance the university’s mission. It’s a payment for the role Harvard plays in advancing the research mission of the United States." www.nytimes.com/interactive/...
- Boston is the best city to run in. This is a take I'm willing to fight over.
- Having come up through US academia the rug pull of the last few months has been pretty extreme. Wondering how much longer I (& everyone else) get to do science sucks but with some dead-man's logic it also makes me really appreciate the years that I got to spend asking questions for their own sake.
- Reposted by Richard HeldProud to share our latest paper. doi.org/10.1016/j.cr... Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue. Structural biology in tissue is well and truly here! @rosfrankinst.bsky.social
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- A formidable opponent in the fight against productivity.
- Reposted by Richard HeldNew method toward modeling cells and viruses! HMFF enables integrative membrane modeling and simulation guided by electron microscopy data. Created by Valentin Maurer and Marc Siggel @embl.org @cssbhamburg.bsky.social, in collaboration with @WPezeshkian lab (1/n) www.biorxiv.org/content/10.1...
- Reposted by Richard HeldYesterday, the NIH R35 “Outstanding Investigator” grant to fund scientists in my lab studying antibiotic resistance was terminated for reasons not related to the content of the science, or any actions taken by me or members of my lab
- Ok microtubule people, what is going on here? Cross section projection images, two different areas and surrounded by intact microtubules:
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- Reposted by Richard HeldWe have updated our #MemBrain v2 preprint with a lot more details about the MemBrain-pick and MemBrain-stats modules, as well as some application examples! Stay tuned for the upcoming thread by lead author @lorenzlamm.bsky.social! 🧠🧵 #CryoET #TeamTomo www.biorxiv.org/content/10.1...
- Reposted by Richard HeldThrilled to share these experiments on shaping focused ion beams to explore different ways of milling. Here, we explore using different beam geometries to generate cellular thin sections/lamellae. The video shows elongating the beam on a charging spot burn www.biorxiv.org/content/10.1...
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- Could run this article for every flight he takes
- Maybe Jeffrey Goldberg is in all of our signal chats and we just have to step back from our lives for a bit to really notice him there.
- Reposted by Richard HeldHere's our newest study: we reveal the molecular principles of neuronal excitation by glutamate, and how physiological temperatures influence this process. www.nature.com/articles/s41... www.nature.com/articles/d41... Led by @anish-mondal07.bsky.social, published in @natureportfolio.nature.com
- Reposted by Richard HeldThere are days in life that shake you. I’m shattered 💔 to share that I just found out that the US Government terminated my 2024 NIH Director’s Early Independence Award (~$2 million), threatening my long-promised assistant professor job at Columbia University & academic career... 1/🧵
- Setting up exciting new crosses like
- Soapbox: acknowledging that the establishment of pax americana is often a morally complicated legacy does not require you to say there is no "wrong side of history." Nuance =/= nihilism and whataboutism is designed to paralyze you into believing you can never act in a principled manner.
- As flying in the US gets increasingly dicey, we will have to fall back on our rail system in order to travel safely. *whispered sidebar* oh I see...
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- Somewhere between this Derek Lowe quote and the Kendrick halftime show, you will find my current vibe
- Reposted by Richard HeldOver on LinkedIn, the head of the Executive Secretariat of the NIH -- a central part of NIH leadership 🧪🩺-- resigned with a lettter worth reading www.linkedin.com/posts/nathan...
- Window shopping for Scientifica rigs like pressing my nose against a Ferrari dealership
- Going to patch for the first time since 2018 (🤯). How long do we think it takes to get a decent trace? Like riding a bike, I'm sure 😬
- Federal employees (even probationary) have rights and should immediately appeal if they are fired. www.mspb.gov
- My small selfish bit of rage – insignificant compared to the existential threats to US science – is that the work of almost half my life is going really well and taking off into new directions and I would love to be happy and optimistic about that.
- Life is offering up an abundance of lemons, therefore:
- Reposted by Richard HeldExcited to share our new preprint "Dynamic nanoscale architecture of synaptic vesicle fusion in mouse hippocampal neurons" www.biorxiv.org/content/10.1... We combine optogenetic stimulation of neurons with in situ cryo-ET to characterize membrane dynamics during synaptic vesicle fusion ❄️🔬⏱️
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- Reposted by Richard HeldThis is the end of science as we know it if it stands—15% IDCs for all NIH Grants. grants.nih.gov/grants/guide...
- Getting ready to fly back to the states after a great conference:
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