Allegretti Lab
Cellular structural biology (cryo-EM/ET, CLEM, biophysics) | nature, society, sport and fatherhood | Group Leader at MRC_LMB | mrclmb.ac.uk/research-leaders/ma…
- Reposted by Allegretti LabWe are excited to share our new preprint which is now available to read on biorXiv: doi.org/10.64898/202... 🎉🎉🎉
- We are thrilled to share our new pre-print on the molecular anatomy of nuclear proteasome in human sperm cells! #In-cellcryo-ET + #SPA + #LM! @piotrkolata.bsky.social @a-dsantos.bsky.social, @tomdendooven.bsky.social!! doi.org/10.64898/202...
- We discovered giant membrane-less islands of proteasomes in human sperm nuclei, containing 20S and 20S-PA200 populations! We solved their in situ structure at sub-nanometer resolution (up to 6.8 Å) and in vitro at near-atomic resolution (1.8 Å)!
- Reposted by Allegretti LabHoly cow the Zhou lab at UCLA is cooking with Isonet 2 - www.biorxiv.org/content/10.6... Averaging-free direct visualization of ribosome translational state! #teamtomo #cryoet
- Reposted by Allegretti LabI’m excited to announce that I will be starting my research group on computational enzyme design at the Generative Biology Institute, Oxford, UK (@eitoxford.bsky.social)! PhD applications are open, deadline is Jan 8th (www.chem.ox.ac.uk/genbio-dtp). Email kiarash.jamali@eit.org with any questions!
- Reposted by Allegretti LabVery sad news, John Gurdon has died. A developmental biologist's developmental biologist, Nobel prize winner His work is the foundation of much of today's dev & stem cell bio. An inspiration to many, including me. Always asking questions & wanting the answers www.magd.cam.ac.uk/news/profess...
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- Reposted by Allegretti LabExciting to see our protein binder design pipeline BindCraft published in its final form in @Nature ! This has been an amazing collaborative effort with Lennart, Christian, @sokrypton.org, Bruno and many other amazing lab members and collaborators. www.nature.com/articles/s41...
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- Reposted by Allegretti LabWe are excited to announce the first in-cell structure of a LINC complex within a native nuclear envelope at subnanometer resolution!! #In-cell cryo-ET + #atomistic MD simulations! @tomdendooven.bsky.social et al!! www.biorxiv.org/content/10.1...
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- Dear all, we are looking for a research assistant in protein biochemistry / cell biology to support our work on nuclear reshaping in human cells. Here the link to the call, please spread: mrc.tal.net/vx/mobile-0/...
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- Reposted by Allegretti Lab🚨 JOB ALERT 🚨 We're hiring a Research Assistant – no PhD needed! Join us to explore the molecular mechanisms of receptor sorting in endosomes. Experience in protein expression/purification, cloning & cell culture desired 🧫🔬 Apply now 👉 www.nature.com/naturecareer... #STEMCareers #ResearchJobs
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- Reposted by Allegretti Lab“The Website” is here! It can be intimidating getting into analysis of #CryoET data, and software is evolving all the time👩💻🚀. @phaips.vd.st & @florentwaltz.bsky.social put our lab’s workflows into an online guide. We hope this helps lower the bar to joining #TeamTomo. Feedback much appreciated! 🧪🧶🧬🔬
- Hey #TeamTomo, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why @florentwaltz.bsky.social and I made this website! tomoguide.github.io Follow the thread 1 /🧵 #CryoET #CryoEM 🔬🧪
- Reposted by Allegretti Lab“Join the Pigino Lab! We’re hiring a PhD student to explore primary cilia biology using advanced imaging, spatial ‘omics, and AI. Apply now at www.cilia-ai.eu and be part of the Cilia-AI Doctoral Network!”
- 👀 Looking for a PhD position but you are torn between cilia biology and computational AI research? 😜 The Cilia-AI Doctoral Network has you covered! Lots of fantastic projects throughout Europe, also @humantechnopole.bsky.social with us or @gaiapigino.bsky.social - apply now! ‼️ www.cilia-ai.eu
- Reposted by Allegretti LabFinally in peer-reviewed form: Mapping the ultrastructural topology of the corynebacterial cell surface. @bupbuse.bsky.social @vikramalva.bsky.social Please also see concurrent beautiful work from @adriasogues.bsky.social and Han Remaut.
- The unique #CellEnvelope of #corynebacteria: @tbharat-lab.bsky.social &co map the C. glutamicum cell surface, revealing a patchy S-layer & specific assembly of the PS2 protein, thereby informing our understanding of cell envelopes that contain #MycolicAcids @plosbiology.org 🧪 plos.io/3Y6JNgw
- Reposted by Allegretti LabExcited to share our new preprint on co-transcriptional splicing! U1 snRNP is recruited to RNA polymerase II through multiple interaction sites, with elongation factor SPT6 binding directly to the U1 snRNP protein. www.biorxiv.org/content/10.1... #cryoEM #transcription #splicing
- Reposted by Allegretti LabHappy to share our manuscript on the in situ visualization of the copia retrotransposon in its final form today published in @cellcellpress.bsky.social www.cell.com/cell/fulltex.... What’s new?
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- Reposted by Allegretti LabWe have an exciting opportunity for a new independent group leader with an interest in structural/molecular biology and machine learning to join our Division of Structural Studies at the @mrclmb.bsky.social! 🥳 Re-posts appreciated. www.nature.com/naturecareer...
- Ever wondered how the chemotherapeutic drug paclitaxel can target slow-dividing tumours? Check out our preprint Hale et al! @tom-hale.bsky.social found a novel non-mitotic mechanism of action for paclitaxel involving nuclear-cytoskeletal coupling via the LINC complex! www.biorxiv.org/content/10.1...
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- Reposted by Allegretti LabTowards community-driven visual proteomics! Excited to finally share this large-scale curated & annotated dataset of 1829 high-quality #cryoET tomograms of the little green alga that just keeps giving— Chlamydomonas! 🧪🧶🧬🌾🌊🌍 Preprint📜: www.biorxiv.org/content/10.1... A short thread🧵👇
- Reposted by Allegretti LabWonderful to see that our colleague @marta-shahbazi.bsky.social is now an EMBO YIP. www.embo.org/press-releas...
- Reposted by Allegretti LabCrosslinks can sometimes improve AlphaFold3 models. Crosslinking is an experimental method often used as a source of distance restraints in modeling. In AlphaFold3, instead of using restraints, crosslinks can be included as covalent ligands. Thanks to Agnieszka Obarska-Kosinska for the idea.
- Reposted by Allegretti LabCongratulations to @marta-shahbazi.bsky.social, Group Leader in @cellbiol-mrclmb.bsky.social, who has joined the EMBO Young Investigator Programme! Read more here about the programme and Marta’s research here: www2.mrc-lmb.cam.ac.uk/marta-shahba... #LMBNews
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- Reposted by Allegretti LabOk, trying to bring my community back together... I created a starter pack for lipids and membrane protein aficionados! Feel free to share, add and distribute, so we can come together again! 😍 go.bsky.app/AEeXh86at://did:plc:sovndmxxrob7lxmbbggn6dgp/app.bsky.graph.starterpack/3lazyh7nbdq2u
- Reposted by Allegretti LabHere is a starter pack for CryoEM/ET enthousiasts To be completed go.bsky.app/JmQksCat://did:plc:pkzh2psjildyroejswbveobs/app.bsky.graph.starterpack/3lahqziu6gr2l
- Join us! Ill present some of our recent tomo work on sperm cells!
- Ever wondered if nuclear pore architecture and function changes during germ cell differentiation? In human spermatogenesis it does, and radically!! Check out our first lab preprint @AliaCSantos et al! www.biorxiv.org/content/10.1...
