Hana Polasek-Sedlackova
Group leader at the Institute of Biophysics, Czech Academy of Sciences | Czech Science Foundation Junior Star fellow | ERA & EMBO IG fellow | Deciphering DNA replication & genome integrity in normal & cancer development.
- Reposted by Hana Polasek-SedlackovaDear Colleagues, Delighted to share that our first lab paper is now published in Nature.!!! 😊😊😊 We identify a previously concealed “safety limit” in DNA replication program: cells intrinsically rate-limit PCNA via PAF15, shaping strand-specific and global replication dynamics. 👉 rdcu.be/e1bBt
- 🚀 Qlife Winter School 2026: Quantitative Approaches to DNA Damage and Repair! Hands-on workshops for Master's, PhD, postdocs & junior scientists. 🗓 Apply by Jan 19, 2026! #QlifeWinterSchool #QuantitativeBiology #LifeScienceTraining
- Reposted by Hana Polasek-SedlackovaThrilled to share our latest work - now available in full in Nature Communications - led in my lab by @vcherdy.bsky.social, with key collaborative contributions from @nitikataneja.bsky.social . rdcu.be/eVrxX
- Reposted by Hana Polasek-SedlackovaHana Polášek-Sedláčková @hanasedlackova.bsky.social from our Institute of Biophysics is one of 28 group leaders who just became @embo.org Young Investigators for the next four years. #LifeSciences www.avcr.cz/en/media/pre... Details ➡️ www.embo.org/press-releas...
- Thrilled to be selected as one of the 28 EMBO Young Investigators for 2026! It is an incredible honor, and I am especially proud to be the second scientist from Czechia chosen since the programme began in 2001. Happy to join Tomas Pluskal in representing our country in the EMBO YIP.
- Congratulations to the new cohort of 28 EMBO Young Investigators! They are joining an international network of more than 800 life scientists – Welcome! Read the press release here: embo.org/press-releases/twen…
- Huge thanks to all my mentors, supervisors, colleagues, my lab, and the Institute of Biophysics! Grateful beyond words. 💚✨
- Truly delighted to host @agagambus.bsky.social next week in Brno for an IBP Guest Lecture! Her groundbreaking work and genuinely human approach to science have always been a great inspiration to me. Come and join us for her lecture at IBP 👇
- 📢 Excited to share that our study has just been published in Nature Communications! We uncover how CRL4-DCAF12 regulates the cellular levels of MCMBP, a chaperone essential for assembling nascent MCM2-7 complexes, to ensure accurate and error-free genome duplication.
- 💡 Our previous work showed that MCM equilibrium is maintained by two distinct protein pools – parental and nascent MCMs (www.nature.com/articles/s41...). In this new study, we reveal how CRL4-DCAF12-mediated degradation of MCMBP provides a new quality control step in this process.
- 👉During MCM biogenesis, MCMBP facilitates the assembly and transport of newly synthesized MCM subcomplexes into the nucleus.
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View full threadRead the full article here: www.nature.com/articles/s41...
- Reposted by Hana Polasek-SedlackovaA comprehensive genetic catalog of human double-strand break repair | Science www.science.org/doi/10.1126/...
- Reposted by Hana Polasek-Sedlackova@natsmb.nature.com has published three pieces on scientists returning to their country of origin after working abroad. www.nature.com/nsmb/article... Two more will come out. The most recent one by @hanasedlackova.bsky.social hits home for me, a very interesting read. www.nature.com/articles/s41...
- Reposted by Hana Polasek-SedlackovaICYMI: New online: Returning home: there and back again in Czechia
- 📢 Excited to share my World View article, just published in @NatureSMB, where I reflect on returning to Czechia and building research group grounded in collaboration🤝, resilience and curiosity💡. www.nature.com/articles/s41...
- This journey has been possible thanks to kind support of my mentors, dedicated colleagues, generous funding #GACR, #ERAfellowship, #EMBO and inspiring environment at our institute! 🙏
- 👉 There are many chapters and experiences behind this journey. This article touches only some of them. If you are considering establishing lab in Czechia feel free to reach out.
- Special thanks to belongs to editorial and production team of @natsmb.nature.com for their excellent work highlighting this important topic! ✨️
- Reposted by Hana Polasek-Sedlackova🧑🔬🎙️ When you publish with JCB, you can reach more of your community by submitting a short video! Want to promote your research in your own voice? 🎦 See our guidelines: rupress.org/jcb/pages/vi... ▶️ Watch video summaries from our authors: www.youtube.com/@JCellBiol
- Reposted by Hana Polasek-SedlackovaAnother reason to consider HaloTag as an alternative to GFP for tagging
- A Biotin-HaloTag ligand enables efficient affinity capture of protein variants from live cells. New tools developed by Anoop Kumar Yadav, Abhijeet Jadhav, Hana Polasek-Sedlackova @hanasedlackova.bsky.social and colleagues: rupress.org/jcb/article/... #Technology #Chromatin #epigenetics #CellCycle
- Reposted by Hana Polasek-SedlackovaWe're very proud that our recent PhD graduate (and now postdoc) Rosie Wilkins' study on RNase H2 overexpression in human cells has been published in Oncogene. www.nature.com/articles/s41...
- Reposted by Hana Polasek-SedlackovaA Biotin-HaloTag ligand enables efficient affinity capture of protein variants from live cells. New tools developed by Anoop Kumar Yadav, Abhijeet Jadhav, Hana Polasek-Sedlackova @hanasedlackova.bsky.social and colleagues: rupress.org/jcb/article/... #Technology #Chromatin #epigenetics #CellCycle
- Reposted by Hana Polasek-SedlackovaThank you, Melina, for the interview!
- 📢 For all HaloTag lovers💙💚💛❤️💜🩶 we are excited to share that our work, in which we developed a new biotin-HaloTag ligand, is now published in @jcb.org! Full link here: rupress.org/jcb/article-...
- 👉 We showed that strategic adjustment of the linker length is an effective strategy in tailoring HaloTag ligands.
- 👉 This was achieved by a combination of in silico design, chemical synthesis, and cell biology.
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View full thread🙏 We also thank all our founders #GACR, #ERAfellowship, #EMBO, and last but not least, the editors and production team of @jcb.org!
- Reposted by Hana Polasek-SedlackovaYadav, Jadhav, @hanasedlackova.bsky.social et al present a novel cell-permeable biotin-HaloTag ligand. The reported ligand expands the HaloTag toolbox by enabling in vivo labeling & providing capabilities for efficient affinity capture of protein variants from live cells rupress.org/jcb/article/...
- Reposted by Hana Polasek-SedlackovaIn @jcb.org, the @hanasedlackova.bsky.social lab presents a novel cell-permeable biotin-HaloTag ligand. The reported ligand expands the HaloTag toolbox by enabling in vivo labeling & providing capabilities for efficient affinity capture of protein variants from live cells rupress.org/jcb/article/...
- Reposted by Hana Polasek-SedlackovaIt is finally out 👍😀. Great job of the team (mostly absent from Bsky) and @m-saponaro.bsky.social CDK-driven phosphorylation of TRAIP is essential for mitotic replisome disassembly and MiDAS url: academic.oup.com/nar/article/...
- Reposted by Hana Polasek-SedlackovaApply to our PhD program!!
- 🧬 Looking for a PhD position in molecular or developmental biology? 🔬 ✉️ The Hubrecht Institute now has its very own PhD program! Applications are open and will be accepted until September 15th. Read more here 👉 www.hubrecht.eu/hipp/
- Reposted by Hana Polasek-SedlackovaFinally out in Nature Chem Bio: SNAP-tag2 for faster and brighter protein labeling www.nature.com/articles/s41... Thank you Steffi and Veselin.
- Reposted by Hana Polasek-SedlackovaThis review discusses the cellular pathways mediating Targeted Protein Degradation approaches and points to some gaps in our understanding of the biology of novel therapeutic tools such as PROTACs. doi.org/10.1038/s420...
- Reposted by Hana Polasek-SedlackovaThis editorial details why we think it's a good idea to standarise reporting of microscopy data and what we are doing towards that. I also feel that it is both important and a step towards helping the community. www.nature.com/articles/s41...
- Reposted by Hana Polasek-SedlackovaPostdoc positions open in my lab (Francis Crick Institute) to study 1. initiation of DNA replication. 2. chromatin replication/epigenetic inheritance. Great for biochemists, biophysicists and cryo-EM/cryo-tomography scientists. Deadline 3 August 2025. crick.wd3.myworkdayjobs.com/External/job...
- Reposted by Hana Polasek-SedlackovaIf you are interested in working on chromatin and oogenesis in the mouse in beautiful Copenhagen, we are recruiting a postdoc! jobportal.ku.dk/videnskabeli... Please share!
- Reposted by Hana Polasek-Sedlackova📣Check out our recent review (written with Martina Huranova lab) on converging mechanisms controlling #cilia #dynamics during #cell #cycle, #cell #signaling, and cross-talk of ciliary modules. rdcu.be/epVDc
- Reposted by Hana Polasek-SedlackovaSo excited to share that we @natsmb.nature.com are hiring a new editor! If you are interested about working with an intelligent, compassionate and funny team helping scientists publish great research, please consider applying. Deadline June 20th. springernature.wd3.myworkdayjobs.com/SpringerNatu...
- Reposted by Hana Polasek-SedlackovaNext Tuesday's webinar will feature @agagambus.bsky.social and @chowdhurylab.bsky.social. Join us on June 3rd at 5PM Rome / 11AM NYC for a lively discussion on DNA replication and repair! Zoom link: uniroma1.zoom.us/j/95149984403 Become part of our community: docs.google.com/forms/d/e/1F...
- Reposted by Hana Polasek-SedlackovaOh wow. Starting grant 0-10 years post PhD from 2027, Consolidator 5-15
- Planning to apply for #research #funding from the ERC? From the next application rounds, expect changes to the: • proposal structure • evaluation process • extra funding you can request • eligibility for Starting & Consolidator #Grants (from 2027) More 👇 europa.eu/!RPHWvv
- Reposted by Hana Polasek-SedlackovaLastly, many thanks to Sarah Aitken (@s-j-aitken.bsky.social) and Emma Reilly for writing a great News & Views article that summarizes our findings and puts them into perspective. You can find it here: doi.org/10.1038/d415...
- Reposted by Hana Polasek-SedlackovaDelighted to share that the results of my main postdoc project are now published in @nature.com 🔗 doi.org/10.1038/s415...
- Reposted by Hana Polasek-SedlackovaExciting PhD project available in our lab (time sensitive)! 👇
- Reposted by Hana Polasek-SedlackovaWant to learn more about chromatin and its dynamics? Read this review by Charlotte and Jolijn in our lab, aimed at giving an overview on this and the ways this is dealt with during transcription, replication and damage repair. Thanks to Biochemistry for inviting us! doi.org/10.1021/acs....
- Reposted by Hana Polasek-SedlackovaAlso in Issue 8: - Research Highlights on tracking leukocytes, trap formation and pluripotency transitions - Interview with @hanasedlackova.bsky.social - In memoriam – Angelika Noegel - Epithelial cell extrusion at a glance journals.biologists.com/jcs/issue/13...
- Reposted by Hana Polasek-SedlackovaWe are looking for a motivated postdoc to study genomic imprinting in barley. @apecinka.bsky.social @beata-strej.bsky.social
- Truly honored that my career path and laboratory were featured in the 'Cell Scientist to Watch' interview series. A huge thank you to Sara Morais da Silva and @jcellsci.bsky.social for this wonderful opportunity 🙏
- Cell scientist to watch – Hana Polasek-Sedlackova @hanasedlackova.bsky.social In this interview, we learn about Hana’s career, her insights on collaborative research and her engineering hobby. journals.biologists.com/jcs/article/...
- Reposted by Hana Polasek-Sedlackova
- Reposted by Hana Polasek-SedlackovaOur new preprint is now on bioRxiv! 📄 We introduce qAID, an AI-based workflow for automated analysis of DNA replication dynamics. Glad to have contributed a small part to this study.
- 📢 For all DNA fiber enthusiasts! 🟥🟩 We are thrilled to announce the release of our long-awaited AI-based workflow for the automated and quantitative analysis of DNA fibers. It's an exciting step forward—check it out on bioRxiv! lnkd.in/dQWUPtsP
- 👉 The scanR-based workflow enables automated imaging and multiparameter analysis of thousands of DNA fibers within minutes. ⏱️
- 👉 The analysis provides automated measurements of essential parameters, including DNA fiber frequency, length, and symmetry. 📊
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View full thread📌 Get in touch if you are interested in using workflow in your lab. 📌
- Reposted by Hana Polasek-SedlackovaThrilled to share our lab's first preprint, led by an exceptional PhD student Gita Chhetri and with the support of wonderful collaborators, we find that replisome function is rate-limited by strand-specific PCNA dynamics. Hope you will enjoy reading. www.biorxiv.org/content/10.1... Brief teaser:
- Reposted by Hana Polasek-SedlackovaHow are chromosome breaks repaired and how is their repair influenced and facilitated by nuclear and genome dynamics? Fantastic initiative and collaboration with @karimmekhail.bsky.social, @chiololab.bsky.social, and @lablegube.bsky.social. @natrevmcb.bsky.social Link to article: rdcu.be/edViW
- Reposted by Hana Polasek-SedlackovaExciting seminar series from Genome integrity italian Network (GiiN), chaired by myself and Raffaella Di Micco! Short talks (2x30’), at convenient time for EU/USA attendees. Big shots and raising stars in an expanding field. See flyers + link to receive email alerts. Please repost to your network!👇
- Had an absolutely fantastic time in Essen! Thank you so much, Dominik Boos, for kind invitation and the inspiring discussions that sparked many ideas💡Hemmo Meyer, Michael Ehrmann, Boris Pfander, Simon Popsel, @hellerschmiedlab.bsky.social & students & postdocs 💚 I appreciated every moment 🙏
- Join us tomorrow, February 18, for an exciting @crc1430.bsky.social seminar at @unidue.bsky.social! We’re happy to welcome @hanasedlackova.bsky.social, who will present on 'Molecular Homeostasis of DNA Replication Origins in Human Cells.' #DNAreplication #ResearchCollaboration
- Reposted by Hana Polasek-SedlackovaJoin us tomorrow, February 18, for an exciting @crc1430.bsky.social seminar at @unidue.bsky.social! We’re happy to welcome @hanasedlackova.bsky.social, who will present on 'Molecular Homeostasis of DNA Replication Origins in Human Cells.' #DNAreplication #ResearchCollaboration
- Reposted by Hana Polasek-SedlackovaOur latest work: how can compartmentalization emerge in a eukaryotic genome lacking canonical heterochromatin? By investigating bacterial genomes put in yeast, we show that the presence or absence of transcription is sufficient! #chromatin #3Dgenome #generegulation www.science.org/doi/10.1126/... 👇
- Reposted by Hana Polasek-Sedlackova4-year PhD studentship available in my lab to study helper helicase function in eukaryotic DNA replication. Happy to receive any enquiries directly to me. Come join us and add your figure to the lab lego collection! institute-genetics-cancer.ed.ac.uk/igc-graduate...
- Reposted by Hana Polasek-SedlackovaDead line is approaching. Have a look on the prg.
- Please RT. Join the sixth edition of our Training Unit @institut_curie international course on Genome Instability & Human Disease open to master students, PhDs and researchers! Amazing program. Registration dead line 2nd March 2025 at training.institut-curie.org/courses/geno...
- Reposted by Hana Polasek-SedlackovaPlease RT. Join the sixth edition of our Training Unit @institut_curie international course on Genome Instability & Human Disease open to master students, PhDs and researchers! Amazing program. Registration dead line 2nd March 2025 at training.institut-curie.org/courses/geno...
- Reposted by Hana Polasek-SedlackovaLook forward to welcoming all participants in Frankfurt for some exciting science, building networks and having a good time 🤗🍀
- Reposted by Hana Polasek-SedlackovaI have received a Wellcome Discovery Award to study the initiation of eukaryotic DNA replication, with 8 years of funding at the Francis Crick Institute. Looking for a postdoc in molecular mechanisms, cryo-EM, cryo-FIB and in situ structure? Contact me now! 3 postdoc positions advertised soon.
- Reposted by Hana Polasek-SedlackovaThree must read papers for PhD students. #scisky #PhD #science #research #academicsky 1. The importance of stupidity in scientific research Open Access journals.biologists.com/jcs/article/...
- Reposted by Hana Polasek-SedlackovaAgeing of stem cells reduces their capacity to form tumours #Nature @natureportfolio.bsky.social "Most human cancers initiate at a young age, highlighting the importance of directing cancer prevention efforts towards young individuals." www.nature.com/articles/s41...
- Reposted by Hana Polasek-Sedlackova
- Reposted by Hana Polasek-SedlackovaTTF2 promotes replisome eviction from stalled forks in mitosis https://www.biorxiv.org/content/10.1101/2024.11.30.626186v1
