Francesco Scavone
Biology @Stanford | Kopito lab | ubiquitin & UBLs, UFM1, ribosome, protein quality control, autophagy, endoplasmic reticulum | Foodbank volunteer | 🐘:@scavone@mas.to
📷: Sicilian landscape
- Reposted by Francesco ScavoneI’m excited to share my first-author paper, with co-first author @rongqinxiaoxiao.bsky.social, now out in @nature.com. We developed a live-cell single-particle tracking platform to see how TRiC & prefoldin engage proteins during co- and post-translational folding. 1/9 www.nature.com/articles/s41...
- Reposted by Francesco Scavone🚀 Just published: New1 shields mRNAs from no-go decay — by preventing ribosome crashes at specific codons. What’s new? New1 (eEF3 homolog) stops ribosomes from stalling at C-terminal AAA/AGG/CGU codons. No New1 = collisions + Hel2 recruitment + Cue2-mediated no-go decay.
- Reposted by Francesco ScavoneStructure of the adaptor protein 4 (AP-4) complex revealed! AP-4 exists in a dynamic equilibrium between closed and open conformations in solution. ARF1 does not shift this equilibrium but is required—together with cargo—for membrane recruitment. rdcu.be/e1ozM
- Reposted by Francesco ScavoneAfter a long review process, I'm excited that our paper is finally in print: www.cell.com/cell/fulltex... TL;DR: We use CRISPR screens in iPSC-derived neurons to find a new tau E3 ligase and a relationship between oxidative stress, the proteasome, and tau proteolytic fragments. More below 👇
- Reposted by Francesco ScavoneOur paper is (finally) out in Cell today! CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis www.cell.com/cell/fulltex... Great collaborative effort - read more from first author @asamelson.bsky.social below:
- After a long review process, I'm excited that our paper is finally in print: www.cell.com/cell/fulltex... TL;DR: We use CRISPR screens in iPSC-derived neurons to find a new tau E3 ligase and a relationship between oxidative stress, the proteasome, and tau proteolytic fragments. More below 👇
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- Reposted by Francesco Scavone‼️Towards the end of #AusUb25, we caught up with Jonathan Pruneda to discuss ubiquitin signalling and its relationship with host-pathogen interactions. It was an amazing conference and such a great chance to meet people from across the ubiquitin field 🤩
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- Reposted by Francesco ScavoneAbsolutely honoured to see our work featured on this month’s cover! 🎉🧬 Artwork by the incredibly talented @doroteaartscience.bsky.social 👇
- 🏖️Our August issue's out! Cover: Flexibility of #autophagy initiation 👉🏼Review on #metabolism in #embryogenesis #devbio 👉🏼Review on #DNArepair 🔬 #ribosomes #neurodevelopment #mitophagy #ferroptosis #stemcells #epigenetics #leukemia #antitumor immunity & more! www.nature.com/ncb/volumes/...
- Reposted by Francesco Scavone
Proud to share what we hope will be a reference study on human oocyte proteostasis. Young-donor eggs show globally reduced organelle activity vs. somatic cells, with an even steeper drop as they mature. 👇 for @zaffagg3 's detailed explanation www.embopress.org/doi/full/10....- 📣 Paper alert!! Excited to share the 2nd research paper from my time in the @bokelab.bsky.social at @crg.eu, out today in the @embojournal.org! We asked: "how do human oocytes regulate proteostatic organelles under physiological conditions?" 1/
- Reposted by Francesco Scavone#cryoEM confluence in Munich #womeninSTEM
- Reposted by Francesco Scavonenew preprint alert from the lab: bringing some order into the wide and wild diversity of archaeal histones. Who has acidic histones? who has multiple histones? what histone combinations do archaea employ and why? find all of this and more here www.biorxiv.org/content/10.1...
- Reposted by Francesco ScavoneThe ESCRT complexes are a fascinating membrane cutting machine with roles in countless cell pathways, but they are also ubiquitous in disease and offer new opportunities as drug targets. See our new review with Alyssa Coyne, Marta Miączyńska, and Harald Stenmark at tinyurl.com/yex3trem
- Reposted by Francesco ScavoneCheck out our latest preprint with the Qi lab on the structure and disease physiology of the human ERAD complex. Outstanding work by Leo, a cell physiologist whom we successfully tempted into structural biology! Preprint: www.biorxiv.org/content/10.1...
- Highlight on our recent work on UFMylation by @dimitristypas.bsky.social on @NatureSMB: www.nature.com/articles/s41...
- Reposted by Francesco ScavoneHuge thanks to @gthibault.bsky.social and Julie Hollien for organizing a warm, welcoming FASEB ER meeting filled with incredible science! Excited that I will carry the torch as a co-organizer for the 2027 meeting….can’t wait! @faseborg.bsky.social
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- Reposted by Francesco ScavoneThrilled this paper is out! We solved the first cocrystal structure of FSP1 with an inhibitor (FSEN1), providing mechanistic insight & a foundation for medchem. Led by Amalia Megarioti & Sitao Zhang. A terrific collaboration with Da Jia. Brief Skytorial! 1/9
- Reposted by Francesco ScavoneExcited to present first publication from the Joshi lab, now in @jcb.org Here, we show that phosphatidic acid regulates Pex30 distribution at ER subdomains for lipid droplet biogenesis by binding to dysferlin domain. rupress.org/jcb/article/...
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- New paper from our lab! 🥳🎉 "UFMylation orchestrates spatiotemporal coordination of RQC at the ER" @Science Advances www.science.org/doi/10.1126/...
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- Reposted by Francesco ScavoneOur PKA-autophagy story by outstanding graduate student Ashley Segura (co-led with Rose Citron) published in @embojournal.org, dissects a deep connection between autophagy and PKA in neuronal signaling, with implications for neurological disease. Great collaboration with @manciaslab.bsky.social!
- AKAP11 recruits PKA holoenzymes to #autophagosomes in a bidirectional functional association, both promoting downstream PKA signaling and increasing AKAP11-PKA complex degradation @robzonculab.bsky.social @ucberkeleyofficial.bsky.social www.embopress.org/doi/full/10....
- Reposted by Francesco Scavone"The bridge-like lipid transport protein VPS13C/PARK23 mediates ER–lysosome contacts following lysosome damage" out now in Nature Cell Biology rdcu.be/ehbaL
- Structure of a Gcn2 dimer in complex with the large 60S ribosomal subunit www.pnas.org/doi/abs/10.1...
- Highly conserved ribosome biogenesis pathways between human and yeast revealed by the MDN1-NLE1 interaction and NLE1 containing pre-60S subunits academic.oup.com/nar/article/...
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- Reposted by Francesco ScavoneHappy to have contributed in a small way to this spectacular study from @aakritijain.bsky.social & @robzonculab.bsky.social identifying LyLAP as a protease for TM domains in the lysosome. Upregulated in PDAC, LyLAP safeguards lysosomes from membrane damage. www.science.org/doi/10.1126/...
- Reposted by Francesco ScavoneFantastic study out today by superstar postdoc @aakritijain.bsky.social! It identifies the unresolved function of LyLAP, a protein associated with poor prognosis in PDAC. It is not a phospholipase but a membrane protein protease! Happy that I could help through our untargeted lipidomics platform
- SO excited to share that a major part of the postdoctoral work in @robzonculab.bsky.social lab is now published: “Leucine aminopeptidase LyLAP enables lysosomal degradation of membrane proteins” www.science.org/doi/10.1126/...
- Reposted by Francesco ScavoneBryce Brownfield's review on the structural biology of Golgi trafficking has been published! authors.elsevier.com/a/1kqrY3PA3s... Bryce is now a postdoc in the @buzbarstow.bsky.social lab, we miss him dearly!
- Reposted by Francesco ScavoneSuper excited to share our latest work on deciphering the #Ubiquitin Code “𝗨𝗯𝗶𝗥𝗘𝗔𝗗 𝗱𝗲𝗰𝗶𝗽𝗵𝗲𝗿𝘀 𝗽𝗿𝗼𝘁𝗲𝗮𝘀𝗼𝗺𝗮𝗹 𝗱𝗲𝗴𝗿𝗮𝗱𝗮𝘁𝗶𝗼𝗻 𝗰𝗼𝗱𝗲 𝗼𝗳 𝗵𝗼𝗺𝗼𝘁𝘆𝗽𝗶𝗰 𝗮𝗻𝗱 𝗯𝗿𝗮𝗻𝗰𝗵𝗲𝗱 𝗞48 𝗮𝗻𝗱 𝗞63 𝘂𝗯𝗶𝗾𝘂𝗶𝘁𝗶𝗻 𝗰𝗵𝗮𝗶𝗻𝘀” @cp-molcell.bsky.social 1/8 www.cell.com/molecular-ce...
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- Reposted by Francesco ScavoneI wrote about the harmful impacts of NIH funding cuts on the health and economy of Indiana. www.indystar.com/story/opinio...
- Reposted by Francesco ScavoneExcited to share our latest work! We found kinesin Kar3 is required for ER protein quality control. This 10-yr project involved 78 students, including undergrad & master's students in my lab & students enrolled in CURE I helped design, & 2 excellent colleagues. www.molbiolcell.org/doi/10.1091/...
- Reposted by Francesco ScavoneMitochondria a safe haven for aggregation-prone proteins? #neurodegeneration. #Parkinson. Find our latest manuscript in @biorxivpreprint.bsky.social www.biorxiv.org/content/10.1...
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- Here is the link to the final version of our manuscript on K63-polyubiquitin and ATG9A in membrane trafficking.(molbiolcell.org/doi/10.1091/...)
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- Our work on K63-polyubiquitin in membrane trafficking is out! Check out how we use a high affinity and specific sensor for K63-polyUb to identify K63-polyubiquitylated plasma membrane proteins in clusters of vesicles containing ATG9A. Link: www.molbiolcell.org/doi/10.1091/...
- What a journey... 🚀 Many thanks to Sharon Lian for all the help with experiments and Tingting Yao and Bob Cohen for their mentoring and funding. The beautiful CLEM images were obtained by Evaa-Liisa Eskelinen.
- Reposted by Francesco ScavoneAutophagy UK & Proteostasis UK joint meeting -June 3-5. Exploring the lifecycle of cellular components from synthesis to degradation. Going to be a great meeting and we look forward to welcoming you to Dundee! Exciting lineup of speakers and opportunities for ECRs Register here: tinyurl.com/mr3p49f9
- Really cool work identifying RIOK3 as a Ubiquitin-binding factor for 40S ribosome degradation: www.cell.com/molecular-ce...
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- Reposted by Francesco ScavoneToday in @nature.com we share our back-to-back stories with Ning Zheng’s lab revealing chemical-genetic convergence between a molecular glue degrader & E3 ligase cancer mutations. 1/5
- Reposted by Francesco ScavoneDear #plantsci community, PLEASE HELP or RT! We are looking for a leaf specific promoter for a tissue specific CRISPR experiment in Arabidopsis. Any suggestions?
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- Reposted by Francesco ScavoneIt’s incredibly hard to study lipids in biological membranes on the nanoscale. You need near-perfect information on both membrane ultrastructure and lipid density, which usually isn’t attainable. Lipid-CLEM brought to you by @mathilda95.bsky.social changes that. www.biorxiv.org/content/10.1...
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- Cool model of polyubiquitin chain length dictating ribosome stall resolution rdcu.be/d8iMr #ribosome #RQC
- Long-term imaging of individual ribosomes reveals ribosome cooperativity in mRNA translation: Cell www.cell.com/cell/fulltex...
- Reposted by Francesco ScavoneAmazing stuff from @marvintanenbaum.bsky.social! Long-term imaging of individual ribosomes reveals ribosome cooperativity in mRNA translation: Cell www.cell.com/cell/fulltex... #RNAsky #RNABiology
