Juan S. Bonifacino
Cell biologist at NIH researching mechanisms of protein and organelle transport, and related diseases. Special interest in neurons and neurodevelopmental disorders. Passionate about science, nature, travel, and music. Views are my own.
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- Structure of the adaptor protein 4 (AP-4) complex revealed! AP-4 exists in a dynamic equilibrium between closed and open conformations in solution. ARF1 does not shift this equilibrium but is required—together with cargo—for membrane recruitment. rdcu.be/e1ozM
- Highly recommend! Great mentor and environment.
- Our wonderful technician Xiaolin (Lin) Zhu is retiring after 27 years of dedicated service in our lab. We will miss her greatly, but we are happy for her as she begins this well-deserved new chapter in her life.
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- This year I had the chance to see Edvard Munch’s The Sick Child painting in Oslo. It’s a powerful reminder of what our Institute, NICHD, NIH, works to prevent and cure.
- Reposted by Juan S. BonifacinoMy lab in the NIH Intramural Program (Bethesda, MD) will be recruiting postdoctoral fellows over the next year with flexible start dates. We work on nanoscale cellular imaging of the plasma membrane and related organelles. Please reach out if you’re interested. www.training.nih.gov/jobs/pdp-053...
- Latest preprint from our lab reports that the distinct pH of anterograde (less acidic) and retrograde (more acidic) lysosomal vesicles in the axon depends on assembly of the V1 and V0 domains of the vacuolar H+ ATPase, mediated by the metazoan RAVE complex www.biorxiv.org/content/10.6...
- Interesting findings linking RAB14 to the EARP complex and the lipid transfer proteins SHIP164 and UHRF1BP1 on endosomes!
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- Excited to share our latest manuscript by @saikat2025.bsky.social and an international team, reporting variants in the EARP- and GARP-interacting protein EIPR1 associated with a neurodevelopmental disorder. Will send you the PDF if you can’t access the article: academic.oup.com/brain/articl...
- Big thanks to Amelia Glazier and @jcellsci.bsky.social for including me in ‘First Persons’ through the decades – 100 years of notable scientists in Journal of Cell Science journals.biologists.com/jcs/article/... Happy to be in such great company!
- Thank you, @mishtudey.bsky.social and @cp-cellchembiol.bsky.social for featuring this interview with @depaceraffa.bsky.social and Chad Williamson in connection to our article on BLOC-1 and BORC dlvr.it/TN9mpn
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- Reposted by Juan S. Bonifacino🚨🚨🚨 Please repost We are looking for postdocs to join our lab at NIH. Apply: www.nichd.nih.gov/research/atNICHD/Investigators/rocha/apply Learn more about training at NIH : www.training.nih.gov/research-tra...
- Excited to share our new review with @depaceraffa.bsky.social, @saikat2025.bsky.social, and Chad Williamson on the BLOC-1 and BORC complexes—key regulators of endolysosomal processes and linked to several genetic diseases. #NIH #Lysosomes #RareDiseases authors.elsevier.com/a/1lg4i8jWWJ...
- Happy to share an exciting study from Yihong Ye’s lab at NIH, with a minor contribution from our lab: ceroid lipofuscinosis-4 (CLN4)-linked DNAJC5 mutations cause lysosomal damage as a driver of neurodegeneration in iPSC-derived neurons. CHIP safeguards lysosomes via microautophagy 👉 rdcu.be/eChof
- New from @depaceraffa.bsky.social from our lab #NICHD #NIH in collaboration with Adeline Vanderver @childrensphila.bsky.social and colleagues reporting mutations in the BLOC1S1 subunit of the BLOC-1 and BORC complexes in children with a neurodevelopmental disorder www.medrxiv.org/content/10.1...
- 👉 New publication ahead of print from our lab: tinyurl.com/ye7pntte We show that the protein SPG21, mutated in hereditary spastic paraplegia 21, localizes to endolysosomes via RAB7A, where it promotes mTORC1-dependent TFEB phosphorylation, reducing expression of a subset of TFEB regulated genes
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- Reposted by Juan S. Bonifacino🚨 If you haven’t seen it yet… 📢 The NIH BioArt Source provides a library of FREE professionally designed illustrations and icons, available for anyone to use. They can be Downloaded in High Definition. Check it out at bioart.niaid.nih.gov
- Nice to meet @manorlaboratory.bsky.social in Canada and learn of his work on label-free imaging!
- Happy to have made a minor contribution to this exciting study by Tasha Morrison, John O’Shea, and colleagues highlighting the key role of glycosphingolipid metabolism in natural killer and CD8⁺ T cell biology. authors.elsevier.com/c/1l0F9_278y...
- Reposted by Juan S. BonifacinoInterested in chemical mechanisms of membrane proteins ?We have an opening for postbac fellows. Please share and repost. Thanks ! Our research focuses on understanding the chemical and cellular mechanisms of integral membrane enzymes and transition metal transporters. bit.ly/42jWXYD. /n
- Recruitment of postbacs & postdocs in the NIH IRP has resumed. I have no additional info beyond what is publicly stated on the website. www.training.nih.gov/research-tra...
- Reposted by Juan S. BonifacinoJob ad is here! Check it out :)
- NIH PostBac program is open again! And I will be hiring! #JobPostComing www.training.nih.gov/research-tra...
- Our lab at the NIH (Bethesda, MD) is looking for postdoctoral fellows to join our team studying the molecular mechanisms of protein trafficking and their links to neurodevelopmental disorders starting on or after 10-1-2025. Send your application to juan.bonifacino@nih.gov. Please share and repost!
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- Reposted by Juan S. BonifacinoWe've been studying mechanisms of resilience that protect lysosomes from damage and wrote a review on the topic. We consider lysosomal stress and damage on a spectrum. Please help me share as this is my first post 😀 and I don't have many followers! 🙏 Open Access: www.embopress.org/doi/full/10....
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- RIP my fellow "porteña" Olivia Hussey. "Born on April 17, 1951, in Buenos Aires, Olivia lived a life full of passion, love, and dedication to the arts, spirituality, and kindness towards animals." www.cnn.com/2024/12/27/e...
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- “Behind the Paper” commentary on our recent publication demonstrating regulation of Golgi PI4P and downstream processes by a SYS1–ARFRP1–ARL5–ARMH3–PI4KB axis go.nature.com/4fKotEg
- Fantastic lecture by Svante Pääbo on archaic genomics, shedding light on human evolution and the genetic basis of diseases www.youtube.com/watch?v=tt4I... 🦴🧬
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- Fascinating study by Anne Simonsen's lab and colleagues reveals the role of BEACH-domain-containing proteins in cargo sorting at recycling and secretory tubular-vesicular compartments hubs.la/Q02XJ4TY0
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- In remembrance of those lost to COVID-19, I created a Spotify playlist featuring songs by musicians who passed away from the disease: open.spotify.com/playlist/6jE.... So much beautiful humanity gone.
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- Reposting @yousufakhan.bsky.social reference to a preprint demonstrating that programmed ribosomal frameshifting generates a PLEKHM2 proteoform that behaves as a constitutively active adaptor for ARL8-dependent coupling of lysosomes to kinesin-1, with collaboration from our lab tinyurl.com/mtrkzdrm
- Excited to share our latest findings on how the phosphoinositide PI4P pool at the trans-Golgi network (TGN) is regulated through a SYS1-ARFRP1-ARL5-ARMH3-PI4KB pathway, in which ARMH3 (also known as C10orf76) acts as an ARL5 effector to activate PI4KB. rdcu.be/d1dZ2
- Interesting paper showing that yeast adaptor protein complex AP-3 lacks "ear" domains, and that the disordered "hinge" domains are required for budding from the late Golgi (equivalent to the mammalian TGN) journals.biologists.com/jcs/article/...
- Reposted by Juan S. BonifacinoA 25-year-old woman with type 1 #diabetes started #producing her own #insulin <3 months after receiving a #transplant of reprogrammed #stem #cells. She is first person with the disease to be treated using cells that were extracted from her own body. #breakthrough? www.nature.com/articles/d41...
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- Reposted by Juan S. BonifacinoThis is very interesting and highly relevant paper to clinical genetics and rare disease researchers. I wrote the accompanying News & Views piece. Free sharing link here: rdcu.be/d0VlK
- This post is about a commentary that I wrote with Xin Yong about recognition of cargo vesicles by tethering factors involved in endosomes to TGN retrograde transport.
- "The evolution of animal stem cells might have involved the exaptation of a pre-existing set of transcription factors", such as Sox, from unicellular organisms rdcu.be/d0NA9
- One positive aspect of moving to Bluesky @bsky.app has been discovering new users to follow, which has broadened my interests. It's refreshing to have a change of perspective from time to time.
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