✨New advance article!
Johansson et al. introduce FLORENCE, Fluorescence labelling in recoded E.coli with non-canonical chemical entities, a SMT labelling method based on genetic code expansion offering an alternative to HaloTag and SNAP-tag systems.
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Optimization of the genetic code expansion technology for intracellular labelling and single-molecule tracking of proteins in genomically re-coded E. coli
Single-molecule tracking (SMT) is a powerful tool for real-time studies of protein interactions in living cells. Dye-labelled SNAP-tag and HaloTag self-labelling proteins have simplified SMT significantly, due to their superior photophysical properties compared to fluorescent proteins. However, due to their
