Jens Schmidt
Cancer Biologist, Husband, Father. Interested in Genomic Integrity, Telomeres, Autophagy, and Single Molecule Microscopy.
theschmidtlab.com
- New pre-print from the lab! Lead by Mariia Mikhova and in collaboration with Kefei Yu’s lab @msumgi.bsky.social we dug into the molecular mechanism of class switch recombination in B cells using simultaneous RNA and protein single-molecule imaging. 1/n www.biorxiv.org/content/10.6...
- CSR is a large scale intrachromosomal deletion that leads to the expression of different antibody isotypes (IgG, IgA, etc.). This deletion requires specific induction of DNA breaks in highly repetitive switch regions that precede the antibody isotype coding regions. 2/n
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- Reposted by Jens SchmidtNew @natcomms.nature.com paper today from Tracy Bryan's lab @cmri.bsky.social: "Nuclear actin and DNA replication stress regulate telomere maintenance by telomerase". Happy our lab could play a small part. Congrats Tracy, Ash Harman, Noa Lamm and the whole team. www.nature.com/articles/s41...
- Reposted by Jens SchmidtNew lab preprint! ERCC6L2 disease is a recessive bone marrow failure syndrome caused by mutations in the putative DNA helicase ERCC6L2. Using mouse genetics, biochemistry and AF3 we uncover ERCC6L2-MRI as a KU-regulatory complex stimulating NHEJ at staggered DSBs: www.biorxiv.org/content/10.1...
- New Print Alert! A fun collab with Kathy Meek at MSU. Led by talented bioinformatician Giovanni Pascarella we demonstrate that Ku70/80 binds intronic antisense Alu elements to inhibit cryptic splice sites. For details see the thread below! 1/n www.biorxiv.org/content/10.1...
- Reposted by Jens SchmidtOur paper in Science is out! @souravagrawal.bsky.social, @rlynn.bsky.social, @susvirkar.bsky.social, and the rest of the team show human RPA is a telomerase processivity factor essential for telomere maintenance. This reshapes our thinking about telomerase regulation. www.science.org/doi/10.1126/...
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- Reposted by Jens Schmidt1/10 Genome maintenance by telomerase is a fundamental process in nearly all eukaryotes. But where does it come from? Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria. www.biorxiv.org/content/10.1...
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- Reposted by Jens SchmidtOur protocol for gene editing is online and all plasmids are on @addgene.bsky.social! CRISPR away everybody and get in touch if you need anything 🤩
- Reposted by Jens SchmidtNew preprint! We solve a mystery you didn't know existed. Mitotic cells lack new transcription but require ongoing translation. Interphase mRNA half life is only 2-4 hrs. So how do cells arrest in mitosis for hours without depleting their transcriptomes? www.biorxiv.org/content/10.1...
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- Reposted by Jens SchmidtThe 3rd Berlin Single Molecule Biophysics Course will run from 8th to 12th September at the HU-Berlin, Institute of Biology. Learn about single channels, single molecule TIRF, theory and analysis. Apply by 7th July at bsmbc@icloud.com. Fee waivers and bursaries available. ! Please share widely !
- Reposted by Jens SchmidtAre you looking for a faculty job? We are hiring in my department at Roswell Park NOW. Contact me for details as I await the formal ad. (Please RT.)
- Reposted by Jens Schmidt🧪 #neurology #neuroskyence tinyurl.com/bp5d8386 Another beautiful example of NIH-SPONSORED bedside to bench and back to bedside work from an outstanding clinician scientist. This work is producing some of the most compelling responses to treatment in what is arguably the worst pediatric brain tumor.
- Reposted by Jens SchmidtNew preprint from the lab @kaelamakins.bsky.social Genetic interactions between 53BP1-RIF1 and DNA-PKcs for DSB repair outcomes. www.biorxiv.org/content/10.1...
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- Reposted by Jens Schmidt🧵1/ 🚨Check out our significantly updated preprint, now out on @biorxivpreprint.bsky.social. Together with Sasi Conte lab, we characterize a novel role for intrinsically disordered regions (IDRs) in promoting RNA-binding selectivity in RNA binding proteins (RBPs): www.biorxiv.org/content/10.1...
- Reposted by Jens SchmidtAnd on other news. My first contribution to science as a postdoc with @stirlingchurchman.bsky.social is out! We discuss the knowns and unknowns of post-transcriptional splicing. It was really fun to write with the great @karinechoquet
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- Reposted by Jens SchmidtHappy to share our latest pre-print! We solved the cryoEM structures of human DNA repair factor MRN bound to DNA and to TRF2. Main work of Yilan, Filiz @filizkuybu.bsky.social and Hengjun. Great collab within the @sfb1361.bsky.social and @genecenter-lmu.bsky.social 🧬🔨 www.biorxiv.org/content/10.1...
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- Thanks @franbottanelli.bsky.social for leading this effort! Addition genome editing plasmids are available on our labs addgene page. www.addgene.org/Jens_Schmidt/
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- Reposted by Jens SchmidtI maxed out my credit card, emptied my savings account, and took out a loan to move from Alabama to Bethesda, MD. I don’t even qualify for unemployment since I’ve only worked at NIH for a month. I will be financially and medically devastated. Seeking suggestions for anywhere that’s hiring!!
- Reposted by Jens SchmidtMaking intrabodies from antibodies just got easier! Learn how we made 𝟭𝟵 intrabodies to bind and light up peptides and histone modifications in live cells. And thanks to Academia, all sequences are freely available. (video credit: Yuko Sato @YukoSatoT2) (1/15) www.biorxiv.org/content/10.1...
- In rare good news these days Hängen-Dasz has brought back pralines and cream! Even better, it was on sale!
- Reposted by Jens SchmidtExcited to share James Jusuf's preprint: By integrating Micro-C with SuperRes Live-Imaging we can calibrate genomics&imaging to perform absolute quantification of looping (e.g. this loop is present 3%) We quantify mESC 36k loops: <loops> are generally rare (2.3%) www.biorxiv.org/content/10.1...
- Reposted by Jens SchmidtWe used cryoET and biophysical approaches in vitro together with molecular biology in differentiated cells to show that chromatin compacted by PRC1-CBX8 is porous and accessible while PRC1 is dynamic in it. 🧪🧬 #cryoET #Epigenetics #Polycomb www.nature.com/articles/s41...
