Hannah Josefine Jüllig

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  Hannah Long hannahlong.bsky.social · Jan 28
  📣 I'm excited to share our latest preprint! We adapt and characterise a neurosphere-based CNCC differentiation protocol, and demonstrate utility for quantitative phenotyping and craniofacial disease modelling! 🧫 Read about Array-CNCC here: www.biorxiv.org/content/10.6... @uoe-igc.bsky.social
  
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• Reposted by Hannah Josefine Jüllig
  
  Ewa ewaoz.bsky.social · Jan 19
  📣 Preprint alert! I am happy to share that our neural crest manuscript is now available on BioRxiv! www.biorxiv.org/content/10.6...

  Array-CNCC: precise aggregation and arrayed plating facilitate quantitative phenotyping of human cranial neural crest cells and craniofacial disease modelling

  Facial development is highly sensitive to genetic and environmental perturbation, with craniofacial malformation associated with over one-third of congenital birth defects. The face arises during an early and largely inaccessible window of embryonic development, with a large contribution from transient and multipotent cranial neural crest cells (CNCCs). Assessment of the molecular and cellular mechanisms driving normal and disordered human facial development therefore relies greatly on the use of in vitro cellular models. Here, we adapted a neurosphere-based CNCC differentiation protocol to facilitate robust quantification of early specification and migration events. Introduction of single-cell aggregation with arrayed plating enabled standardisation of neurosphere size, growth and patterning. Inclusion of fibronectin coating enhanced the efficiency of neurosphere attachment and synchronicity of CNCC migration timing. To demonstrate application of the Array-CNCC method, we developed a strategy for mosaic co-culture, which can facilitate differentiation of wildtype untreated cells directly alongside cells exposed to distinct drug treatments or genetic alterations. Finally, we present a screening approach which we use to test the impact of distinct extracellular matrix components on neurosphere morphology, CNCC migration and gene expression. Together, the Array-CNCC method is highly amenable to quantitative phenotyping and screening approaches, enabling enhanced craniofacial disease modelling with both cellular and molecular readouts. ### Competing Interest Statement The authors have declared no competing interest. Medical Research Council, MC\_UU\_00035/12, MC\_ST\_00035 Wellcome Trust, https://ror.org/029chgv08, 227712/Z/23/Z

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  Hannah Josefine Jüllig hannahjuellig.bsky.social · Nov 22, 2025
  A very nice highlight of our recent paper!
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    Nature nature.com · Nov 20, 2025
    Researchers have identified gene-regulatory variants that might have contributed to Neanderthals’ beefy jaws — offering a window on how the human face developed go.nature.com/3Ke6StJ
    

    Neanderthal DNA reveals how human faces form

    Subtle genomic variations between humans and Neanderthals provide clues to how DNA shapes our facial features.

    go.nature.com
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  Development handle.invalid · Nov 10, 2025
  Neanderthal DNA enhances our understanding of face development This Research Highlight showcases the work from Hannah Long @hannahlong.bsky.social, Kirsty Uttley @kirstyuttley.bsky.social, Hannah Jüllig @hannahjuellig.bsky.social and colleagues: journals.biologists.com/dev/article/...
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  Hannah Josefine Jüllig hannahjuellig.bsky.social · Nov 10, 2025
  Excited to share our new paper out today in Development! Check out Hannah’s fantastic post below for a summary of the findings ✨ journals.biologists.com/dev/article/...
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    Hannah Long hannahlong.bsky.social · Nov 10, 2025
    📣 Paper alert! I am delighted that our paper exploring the impact of Neanderthal-derived variants on the activity of a disease-associated craniofacial enhancer has been published in Development today! journals.biologists.com/dev/article/...
    
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