New preprint 👉Doudna x Bryant x Jacobsen x Savage collaboration!
Work led by
@zehanzhou.bsky.social, I. Saffarian-Deemyad,
@honglue.bsky.social, T. Weiss
We dissect how stepwise DNA unwinding gates TnpB genome editing, revealing how unwound DNA states enhance cleavage
www.biorxiv.org/content/10.6...
Stepwise DNA unwinding gates TnpB genome-editing activity
TnpB is a compact RNA-guided endonuclease and evolutionary ancestor of CRISPR-Cas12 that offers a promising platform for genome engineering. However, the genome-editing activity of TnpBs remains limit...
