Akaash Kumar
Interdisciplinary scientist interested in technology development, microscopy and biological sciences.
Multispectral live cell imaging of membrane trafficking.
MRC LMB, Cambridge, UK.
- 🚨Publication alert🚨 My first, first-author paper is now out in @natphoton.nature.com! Our paper describes an iterative spectral unmixing algorithm and eight-channel camera-based hardware we developed enabling unmixing of low SNR live-cell data at video rates. www.nature.com/articles/s41...
- The technology we developed is compatible with any camera-based microscope and by using polycistronic plasmids and live-cell dyes (e.g. @spirochrome.com) we can image multiple organelles in live cells simultaneously.
- We demonstrated the ability to simultaneously image trafficking of five different endogenous receptor classes using de-novo binders from David Baker's lab, by adding the multispectral technology to a state-of-the-art single objective light sheet microscope, capturing data at 3 volumes per second.
- In future work, I am now using our multispectral technology to study membrane trafficking pathways in cytotoxic T cells and related immunotherapies, to decode how T cells are able to eliminate virally-infected/cancer cells and what happens in patients when these processes go wrong. Updates to come!
- Reposted by Akaash KumarWe present a simple method to easily increase the imageable depth of an expansion microscopy gel on a typical inverted microscope ten-fold, using some carefully placed FEP film and a water dipping objective lens:
- Maximising imaging volumes of expanded tissues for inverted fluorescence microscopy biorxiv.org/content/10.1101/202…
- Today's light sheet video of actin dynamics in cytotoxic t cells... so much fun to come!
- Thank you very much for the feature and the article!! A nice surprise before the weekend...
- New approach with an unmixing algorithm & 8-channel image acquisition enables multispectral live-cell imaging 📷: @akaashkumar.bsky.social et al @jamesdmanton.bsky.social @cellbiol-mrclmb.bsky.social @mrclmb.bsky.social in @biorxivpreprint.bsky.social ➡️: bpod.org.uk/archive/2025... with Ant Lewis
- Congratulations @lara-kruger.bsky.social ! We wish you all the best!
- Lets goo (very soon)... Merci beaucoup to the exquisite @deriverylab.bsky.social at the amazing @mrclmb.bsky.social. I had a lovely, lovely time!
- Reposted by Akaash Kumar🚨 Paper Alert 🚨 Our preprint describing Elongator as a selective microtubule polymerase that rewrites PTMs on microtubules is now published! Check it out: www.embopress.org/doi/full/10....
- Reposted by Akaash KumarCongrats to @juliemiesch.bsky.social who was awarded a Postdoc Mobility grant by @snf-ch.bsky.social to support her research for two years. Her postdoc research will focus on the biophysical regulation of water buffering in the cell.
- Reposted by Akaash KumarMitochondria consist of networks of cylindrical tubes, right? Not necessarily! - in our new preprint, @gavsturm.bsky.social investigates how mitochondria transiently adopt a beads-on-a-string morphology www.biorxiv.org/content/10.1...
- Reposted by Akaash KumarCongrats to @akaashkumar.bsky.social who has won the PhD Award from the Cambridge Centre for Physical Biology for his work developing a novel microscopy system with @deriverylab.bsky.social & @jamesdmanton.bsky.social in @cellbiol-mrclmb.bsky.social. www.physbiol.cam.ac.uk/akaash-kumar #LMBintheNews
- Simultaneous multispectral imaging of seven organelles in live cells using our multispectral spinning disk confocal microscope! #FluorescenceFriday You can read more about this technology in our updated pre-print: www.biorxiv.org/content/10.1...
- Very happy that my work on developing multispectral live cell imaging technology has been recognised by the Cambridge Centre for Physical Biology! Thanks for the award!
- Congrats to @akaashkumar.bsky.social , a postdoc in the lab, for winning the PhD Award from the Cambridge Centre for Physical Biology. His PhD research worked on the development of a multispectral imaging system to image up to 8 fluorophores similtaneously for tackling complex biological questions!
- Reposted by Akaash KumarIn June, @akaashkumar.bsky.social and @jamesdmanton.bsky.social had their Multispectral live-cell imaging paper released on to the BioRxiv www.biorxiv.org/content/10.1...
- Reposted by Akaash KumarThen, in September @akaashkumar.bsky.social passed his PhD viva, becoming the latest doctor to emerge from the lab! He then won the RMS Early Career Award 2024 in October.
- Reposted by Akaash KumarLater in October, the LMB had their yearly annual symposium with Post-doc Lara Kruger winning the Steitz Prize for her talk and @akaashkumar.bsky.social winning the Perutz Prize for his PhD work!
