Akaash Kumar
Interdisciplinary scientist interested in technology development, microscopy and biological sciences.
Multispectral live cell imaging of membrane trafficking.
MRC LMB, Cambridge, UK.
- 🚨Publication alert🚨 My first, first-author paper is now out in @natphoton.nature.com! Our paper describes an iterative spectral unmixing algorithm and eight-channel camera-based hardware we developed enabling unmixing of low SNR live-cell data at video rates. www.nature.com/articles/s41...
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View full threadWe demonstrated the ability to simultaneously image trafficking of five different endogenous receptor classes using de-novo binders from David Baker's lab, by adding the multispectral technology to a state-of-the-art single objective light sheet microscope, capturing data at 3 volumes per second.
- In future work, I am now using our multispectral technology to study membrane trafficking pathways in cytotoxic T cells and related immunotherapies, to decode how T cells are able to eliminate virally-infected/cancer cells and what happens in patients when these processes go wrong. Updates to come!
- The technology we developed is compatible with any camera-based microscope and by using polycistronic plasmids and live-cell dyes (e.g. @spirochrome.com) we can image multiple organelles in live cells simultaneously.
- Today's light sheet video of actin dynamics in cytotoxic t cells... so much fun to come!
- Thank you very much for the feature and the article!! A nice surprise before the weekend...
- New approach with an unmixing algorithm & 8-channel image acquisition enables multispectral live-cell imaging 📷: @akaashkumar.bsky.social et al @jamesdmanton.bsky.social @cellbiol-mrclmb.bsky.social @mrclmb.bsky.social in @biorxivpreprint.bsky.social ➡️: bpod.org.uk/archive/2025... with Ant Lewis
- Congratulations @lara-kruger.bsky.social ! We wish you all the best!
- Lets goo (very soon)... Merci beaucoup to the exquisite @deriverylab.bsky.social at the amazing @mrclmb.bsky.social. I had a lovely, lovely time!
- Simultaneous multispectral imaging of seven organelles in live cells using our multispectral spinning disk confocal microscope! #FluorescenceFriday You can read more about this technology in our updated pre-print: www.biorxiv.org/content/10.1...
- Very happy that my work on developing multispectral live cell imaging technology has been recognised by the Cambridge Centre for Physical Biology! Thanks for the award!
- Congrats to @akaashkumar.bsky.social , a postdoc in the lab, for winning the PhD Award from the Cambridge Centre for Physical Biology. His PhD research worked on the development of a multispectral imaging system to image up to 8 fluorophores similtaneously for tackling complex biological questions!
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- Thank you!
- It's woefully incomplete, but I've made the start of a starter pack for folks interested in microscopy. Suggestions for who else to include (including yourself!) are very welcome. go.bsky.app/SaYfAX3at://did:plc:5t5g4zki76auoldxeo7wkfxt/app.bsky.graph.starterpack/3l4jewk43z32o
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- Hi Mike, @dmikeando.bsky.social could I be added to this please?
- Some personal news: my wife and I are finally homeowners! We got the keys to our home in Cambridge today, very exciting times!
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- Likewise! Took me a while aha hope you're well!
- Interested in microscopy and/or image analysis? Check out my newest starter pack of super folks.(which also links to the previous, 150-awesome-full one! Both also link to other imaging-y starter packs as I find them) go.bsky.app/MU8c2A2at://did:plc:o7tynxmyxsuhoeknkm7r2dul/app.bsky.graph.starterpack/3lavzay5wnr2o
- Would love to be added to this, for all things multispectral microscopy
- My turn to create a starter pack for scientists using live imaging in their research! Of course, I’ve likely missed many—please let me know who else I should include. Thanks! go.bsky.app/KYYr7xc #liveimaging #livevellimaging #microscopyat://did:plc:5wkrpvrno3grto7uyb6qbo5o/app.bsky.graph.starterpack/3lbenbiqtws2i
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- Aha thank you very much!
- Would love to be added. Many updates on all things multispectral live cell imaging!
- Ok, I made a starter pack for membrane trafficking and organelles (broadly defined). Feel free to self-nominate! go.bsky.app/RbtJjt7at://did:plc:qqgx2dooaid3j2qgu4d5xbxc/app.bsky.graph.starterpack/3laoqqodbfc2j
- Thanks for this, super useful for new joiners to bluesky (like me)! Could I be added to this group please?
