Vanderbilt Cell Imaging Shared Resource (CISR)
Vanderbilt Cell Imaging Shared Resource (CISR). Microscopy for all of VU and VUMC. Light microscopy and electron microscopy. Image analysis. medschool.vanderbilt.edu/cisr/
- We're using #FluorescenceFriday to manifest some warmth here in Nashville! Still tens of thousands of people without power, still lots of ice on the roads and trees, and still below freezing. Enjoy this little cell pseudo-colored with Inferno for the nucleus and Orange Hot for the mitochondria. 🔥🧊☀️❄️
- New paper alert for #FluorescenceFriday! Full of confocal imaging from Gu, Jacobson, & Kaverina Labs. "Paracrine Hormonal Signals From Islet α-Cells Regulate Microtubule Dynamics in β-Cells to Promote Insulin Secretion in Mouse and Human Islets" pubmed.ncbi.nlm.nih.gov/41511443/
- First #MicroscopyMonday of 2026! Happy New Year!
- Happy Holidays from the CISR! May all your days and fluorophores be BRIGHT. #MicroscopyMonday
- For the last (workday) #FluorescenceFriday of 2025, let's recap another amazing year in the CISR! We appreciate all our CISR labs, users, faculty, and staff! Thanks for a great 2025, and here's to a great 2026! #Wrapped #Microscopy First, you imaged for 11,082 microscope hours! 1/9
- Looks like the usual suspects for Most Popular Microscopes. 2/9
- CISR staff were busy with microscope trainings! 3/9
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View full threadWe can't wait to continue working with all our CISR researchers in 2026! Happy Holidays! 9/9
- Time for the annual sharing of our favorite Christmas Microscope Joke: The Z-mas Tree! Source unknown. #Microscopy #Confocal #Volume
- Reposted by Vanderbilt Cell Imaging Shared Resource (CISR)Oh where, oh where does my protein localize? Oh where, oh where can it be?🎵 Check out our Subcellular Localization Collection page, with fluorescent protein markers for organelles and cellular structures to help you find your way 🔬
- For #FluorescenceFriday, we're adding #Football. Good luck to Vanderbilt's first ever Heisman finalist, QB Diego Pavia! High-resolution nucleus football to scale (2 microns), Pavia not to scale. #Heisman #DiegoPavia #2Turnt
- Reposted by Vanderbilt Cell Imaging Shared Resource (CISR)The next Special Edition #VirtualPub "Imaging Organoids" will take place on Jan. 30, 1-3 pm CET. Speakers from academia & industry will introduce a range of methods used to image organoid systems from lung buds to mini-brains. Join us! Register 🔽 www.eurobioimaging.eu/special-edit...
- Today is #WorldDiabetesDay & #FluorescenceFriday! One example from Vanderbilt diabetes research. Image from Darian Carroll's graduate work in Gannon lab. NHP pancreas: insulin (green), mitochondria (magenta), DAPI (blue). @healthcare.nikon.com @betacellbelle.bsky.social @vubasicsciences.bsky.social
- Reposted by Vanderbilt Cell Imaging Shared Resource (CISR)Just 4 days until the start of I2K and its 33 totally free image analysis tutorials and events! Please share with your "home networks", especially early career researchers - the videos will be amazing and high-impact no matter how many people attend live, BUT (1/x)
- I think all imaging scientists and microscopy core facility personnel already know we have the greatest jobs in the world, but it's nice to see it written. #microscopy #imaging 🔬😊💯 Vanderbilt friends, come see us to "look at random things through fancy microscopes"!
- Great explanation for #MicroscopyMonday on when to use or not use a Maximum Intensity Projection during your image analysis. "Extracting 3D information from 2D projections is akin to estimating the volume, surface, and content properties form a crushed beverage can"
- Ever wondered if analyzing your images by using intensity Z-projections impacts your data? Happy to announce my guest article in Microscopy & Analysis on that topic. It's a very short read but worth for everyone dealing with image analysis! buff.ly/KJ147tI #Microscopy #ZProjection #ImageAnalysis
- Happy #FluorescenceFriday and #HappyHalloween! Spider web or microtubule web? Let's check the scale bar. 5 microns. Great lesson in the importance of a #scalebar. (Microtubules to scale, spider not to scale) 🎃👻🕷️🕸️
- We're a week behind, but excited to see so many Vanderbilt and CISR researchers with winning images in the 2025 #NikonSmallWorld contest! Congrats to James Hayes (4th, first image here), Dylan Burnette (10th), Zach Sanchez (20th), and Amy Engevik (IOD)! #FluorescenceFriday www.nikonsmallworld.com
- James's and Dylan's images were acquired with the CISR's Nikon SDC SoRa, funded through an NIMH S10 grant. @healthcare.nikon.com @vubasicsciences.bsky.social
- Dylan Burnette (10th) @mag2art.bsky.social
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View full threadAmy Engevik (Image of Distinction). Amy has her own lab now, but we claim her as a former CISR user and Vanderbilt postdoc! @amyengevik.bsky.social
- The CISR is hosting a webinar and demonstration of the Zeiss LSM910 with Lightfield 4D microscope. Join the webinar next Thursday, Oct. 30 at 11 AM. Then contact Jenny Schafer with CISR or Cumberland Dugan with Zeiss to sign up for a demo time the week of Nov. 10. @zeiss-microscopy.bsky.social
- Happy #FluorescenceFriday! Trying out a new color combo for today, Friday, October 3. Mitochondria (MitoTracker) in turquoise, nucleus (DAPI) in orange. Confocal Airyscan, tile, MIP. 🧡💚🔬
- Reposted by Vanderbilt Cell Imaging Shared Resource (CISR)🌍🎗️ Today is #WorldCancerResearchDay. Cancer impacts millions of people around the world and our scientists are working to tackle cancer from every angle: genes, metabolism, drug discovery, and more. Learn more about cancer research being done at our school 👉 medschool.vanderbilt.edu/basic-scienc...
- Reposted by Vanderbilt Cell Imaging Shared Resource (CISR)#HappyFluorescenceFriday! #microscopycommunity- want to learn open source image analysis or share your knowledge to help others? We’ve got a FREE virtual workshop Nov 17-19! Now accepting workshop session applications! Learn more & sign up: buff.ly/esGIotD
- Great opportunity for a "Train the Trainer" workshop for light microscopy facility people! Check it out here. #microscopy #cores
- Join VI4 for Mega Microbe on September 27! This is always a fun event for kids in the Nashville area. CISR-EM will be showing off some cool EM instruments and will show how beautiful these tiny bacteria can be! 🔬🦠🏫
- Looking forward to this joint conference in Montreal! Global BioImaging (GBI) & BioImaging North America (BINA). Registration ends soon! Dr. Jenny Schafer will be representing the CISR. Say hello! #GBIxBINA2025 #BioimagingCommunity #HybridConference #Imaging2025 globalbioimaging.org/exchange-of-...
- Drumroll please 🥁 We're excited to announce the winners of the 2nd Annual CISR Life Is Beautiful Image Contest! 🔬🏅 1 - James Hayes, Burnette/Merryman Labs 2 - Rekha Nagarajan, Tyska Lab 3 - James Costanzo, Gama Lab Read about these researchers, projects, and microscopes here. tinyurl.com/4r6xwe8h
- Tomorrow! First Tuesday of the month. CISR Office Hours. 9:00-11:00 AM. Come see us in 704 Light Hall and/or T-2216 MCN, and ask anything microscopy related!

- Happy #FluorescenceFriday! Submissions have closed, and NEXT WEEK we will be announcing the WINNERS of the 2nd Annual Life Is Beautiful CISR Image Contest! Stay tuned! To get everyone excited, let's enjoy these 3 beautiful winners from last year! 🔬🎨🏆
- Nashville has been under a #HeatAdvisory all week, so how about a #RedHot LUT for #FluorescenceFriday? 🌞😎🥵Might be a cell, might be an artistic representation of what it feels like in Tennessee right now. 🤷
- Hello #MicroscopyMonday! Second, TEM & SEM from the @tyskalabactual.bsky.social lab and first author @operkins.bsky.social with CISR-EM's Dr. Evan Krystofiak of clathrin-coated pits in intestinal crypts (click for more!). @cp-cellreports.bsky.social @vubasicsciences.bsky.social tinyurl.com/yhue25bv
- Hello #MicroscopyMonday! How about 2 new papers with CISR-EM team & Vanderbilt researchers? First, TEM from the @gouldlab.bsky.social with CISR-EM's Dr. Evan Krystofiak to show changes in fungal cell wall architecture. @pnas.org @awillet.bsky.social @vubasicsciences.bsky.social tinyurl.com/23rncw78
- Last day to submit your images to our second annual CISR Life Is Beautiful Image Contest! Submit now. Submit more than one. Share with your lab mates. WIN PRIZES. Happy #FluorescenceFriday! 🔬🧬🎨🏅💰 vanderbilt-university.formstack.com/forms/cisr_l...
- Four days left! Submit your images today! Win prizes! medschool.vanderbilt.edu/basic-scienc...
- Deadline extended! Submit your images to the Second Annual CISR Life is Beautiful Image Contest! New deadline is July 18. Open to anyone with CISR-acquired images. More details here. medschool.vanderbilt.edu/basic-scienc...
- We'll be off enjoying the sunshine 😎 and fireworks 🎆 tomorrow, so here is our contribution to #FluorescenceFriday for the #4thofJuly! What do you see? Fireworks? Red and white stripes? Or just some good ol' Fluo cells? Confocal Airyscan on Zeiss LSM980. 🔬❤️🤍💙🗽
- The submissions are rolling in for our Second Annual Life Is Beautiful CISR Image Contest! Join in by submitting your beautiful image today in celebration of #FluorescenceFriday! Details here. medschool.vanderbilt.edu/basic-scienc...
- Reposted by Vanderbilt Cell Imaging Shared Resource (CISR)Calling all core facilities 🎉- Timely 'Navigating Strategies for Core Facilities 2025: A Town Hall virtual Forum' 16th June. A joint @royalmicrosoc.bsky.social and @msa-microscopy.bsky.social meeting that welcomes all. See - www.rms.org.uk/rms-event-ca...
- For #MicroscopyMonday, brand new paper from frequent lightsheet lab, Matthew Schrag, and first author Lissa Ventura-Antunes. 3D microscopy on human brains with cerebral amyloid angiopathy. Scale 50 µm. Zeiss Z1 lightsheet @zeiss-microscopy.bsky.social pubmed.ncbi.nlm.nih.gov/40465757/
- Ed the Zebra stopped by for some imaging. And I know Ed will be sure to acknowledge the CISR in his upcoming publication. #zebra #microscopy (FYI: There is a pet zebra on the loose in Rutherford County, TN.)
- Reposted by Vanderbilt Cell Imaging Shared Resource (CISR)You have 2 weeks left to submit your scientific images to our 2025 Image Competition! 🗓️ Deadline: Tuesday, 17 June 🏆 Prizes: US$200 gift card, winning image on our journal cover, and a written interview 📄 How to enter >>> www.biotechniques.com/life-science... #Imaging #Microscopy #Bioimaging 🧪
- Happy #FluorescenceFriday! CISR users - don't forget to submit your beautiful images to our Second Annual Life Is Beautiful Image Contest! Deadline is July 1, 2025. Info and submission link here. medschool.vanderbilt.edu/basic-scienc...
- Reposted by Vanderbilt Cell Imaging Shared Resource (CISR)Mark your calendar and spread the word ... @vubasicsciences.bsky.social @vanderbiltvicb.bsky.social @vucellimaging.bsky.social @vandybiomed.bsky.social #structuralbiology #AI #research #cryoem #cryoet #protein
- Happy #FluorescenceFriday! Since we've been on BlueSky for less than a year, we're sharing memories from one year ago in Nashville when we had a #cidada #broodxix invasion! Confocal Z projection (color coded for depth) of a cicada leg. 🔬🐝🍃
- Here we go again! 🔬🎨🏅 The CISR is pleased to announce our SECOND ANNUAL Life Is Beautiful Image Contest! @vubasicsciences.bsky.social Deadline July 1, 2025. Winners announced August 1, 2025. Details and submission link here! medschool.vanderbilt.edu/basic-scienc...
- Reposted by Vanderbilt Cell Imaging Shared Resource (CISR)🎉 Big congratulations to Vanderbilt’s own Heidi Hamm and Eric Skaar on their election to the National Academy of Sciences! 👏 🔗 www.nasonline.org/news/2025-na... #VanderbiltResearch #NAS2025 #ScienceLeadership #TeamVandy #BiomedicalResearch
- Upcoming CISR events! Office hours (EM, Nikon, general light microscopy) and CryoEM discussion group. Join us!
- Reposted by Vanderbilt Cell Imaging Shared Resource (CISR)💻 Announcing the Boston Bioimage Analysis Course — BoBiAC! Join us this July at Harvard Medical School in Boston for a 6-day deep dive into bioimage analysis with Python, hosted by the IAC and CITE. Apply by May 16th, 2025!
- Thank you, @ritastrack.bsky.social! I'll add that it's a REQUIREMENT to acknowledge grants that support cores. Often NIH S10 equipment and NIH P30 center grants. Funding depends on proper acknowledgments. If you need help w/ M&M or acknowledgments, just ask your friendly, local core scientist!

- Your yearly reminder to acknowledge the core facilities you use and their staff scientists in your papers. These scientists are a crucial part of the scientific ecosystem and to continue to exist they need tangible credit for their work. Plus their associated expertise adds credibility to your work.