Daniel Jost - Physical Biology of Chromatin group
CNRS Senior Researcher - Group Leader at Laboratory of Biology and Modeling of the Cell, Ecole Normale Supérieure de Lyon.
Physical biology of chromatin: modeling the spatio-temporal dynamics of eukaryotic genomes.
- 🚨 New preprint from the lab. Combining modeling and data analysis of SisterC data in yeast, we investigate cohesion of replicated chromosomes and show that sister chromatids are loosely and asymmetrically aligned in G2/M. Check the tweetorial below ⬇️ www.biorxiv.org/content/10.6...
- How does cohesin keep sister chromatids together and organize them after DNA replication? Cohesin isn’t just a “glue” for sister chromatids (cohesion function)—it also shapes their 3D organization via loop extrusion. But how do these two functions interact?
- Our study combines: ✔️ Polymer modeling ✔️ Data analysis of Genome-wide contact maps (WT vs. mutants) of SisterC, Hi-C and Micro-C datasets.
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View full thread🤔 Open question: If sister chromatids are partially and asymmetrically aligned, how does the cell ensure accurate repair and segregation?
- SMC and recombination enthusiasts: we updated our work describing the loop extrusion properties of budding yeast condensin and its function in biasing donor usage for mating-type switching. Lots of cool new data, check it out! www.biorxiv.org/content/10.1...
- Our Mini Review went online last night in Current Opinion in Genetics & Development "Block copolymer concepts of how transcription organizes the stem cell genome" doi.org/10.1016/j.gd... Fig. 1: Stem cell–typical organization of the genome and transcription, and the block copolymer sorting concept.
- 🚨Our work on the impact of DNA replication on 3D genome is out in Genome Biology: replication-dependent loop extrusion by sister-forks, wave of replication, no evidence for large-scale replication factory. Great collab with @aurelepiazza.bsky.social. More here: link.springer.com/article/10.1...
- 🚨 new preprint from the lab. Combining modeling, new Hi-C data in yeast and data analysis, our study offers new insights into the spatial and dynamic organization of chromatin during replication in eukaryotes. Check the tweetorial below ⬇️ www.biorxiv.org/content/10.1...
- BIG ANNOUNCEMENT📣: I haven’t been this excited to be part of something new in 15 years… Thrilled to reveal the passion project I’ve been working on for the past year and a half!🙀🥳 (thread 👇)
- New preprint from the lab !! Loop extrusion may provide mechanical robustness to chromatin. Great work by Hossein Salari. @cnrs.fr @lbmcinlyon.bsky.social www.biorxiv.org/content/10.1...
- Chromatin gets stiffer when pulled gently but softer when yanked hard—thanks to loop extrusion. SMCs may play the role fo shock absorbers, protecting the genome from weak mechanical perturbations but allowing adaptation for persistent strong stresses.
- "A la découverte des chromosomes", la conférence immersive imaginée par @djost-physbiol.bsky.social est en ligne sur la chaine Youtube du CNRS. www.youtube.com/watch?v=CHh_... @cnrsbiologie.bsky.social @cnrs-rhoneauvergne.bsky.social @ensdelyon.bsky.social
