Tom McLean
Structural Biochemist. Research Officer at WEHI. Future DECRA Fellow at Monash.
- Reposted by Tom McLeanFirst results chapter from Kelly-Rose Tulley’s PhD is published. It was a great team effort to get this finished. This work supports previous results on S. coelicolor OrrA and suggests its function is highly conserved. #microsky #streptomyces 1/2
- Defining the OrrA regulon and its role in development and antibiotic production in Streptomyces venezuelae NRRL B-65442 biorxiv.org/content/10.64898/20…
- Too excited by the science to include the link to the paper! Here it is: journals.asm.org/doi/10.1128/...
- Delighted to see the main work from my PhD finally published in @mbio.bsky.social! It all started with the observation that deleting the cutRS two-component system in S. venezuelae caused this amazing explorer phenotype in the presence of glucose. But what was going on?! (1/n)
- Delighted to see the main work from my PhD finally published in @mbio.bsky.social! It all started with the observation that deleting the cutRS two-component system in S. venezuelae caused this amazing explorer phenotype in the presence of glucose. But what was going on?! (1/n)
- This mutant also overproduced the antibiotic chloramphenicol, leading to a very potent anti-Gram-postitive activity on plate assays. However, this still didn't give us any clues as to what linked CutRS to the phenotype. (2/n)
- My favourite piece of data in the whole paper came after we quantified the biomass difference between the WT and ∆cutRS mutant on glucose media, showing it is around 4x more massive. It also uses up all the glucose in the plate, close to gram, in just 9 days! (3/n)
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View full threadAfter all that, i just want to thank all the amazing co-authors but particularly @ainsley-beaton.bsky.social for helping me get to the bottom of this story, and my PhD supervisor @matthutchings.bsky.social for always believing in the project!
- Reposted by Tom McLean📄 Delighted to share that the preprint of my main PhD work is out now! 👏 A huge thank you to everyone involved, @tommclean.bsky.social, Govind Chandra, Ngat Tran (both not on BlueSky) and especially my PhD supervisor @tunglejic.bsky.social 👏 🧵 below! www.biorxiv.org/content/10.6...
- Check out this work by the amazing @leahmcphillips.bsky.social from during her PhD!!
- Stable inheritance of the Streptomyces linear plasmid SCP1 by dual ParABS partition systems biorxiv.org/content/10.64898/20…
- Reposted by Tom McLeanOur preprint is now published in PNAS! This came together thanks to a great collaboration with Antoine Hocher and a strong team effort from the Le Lab. Thank you to the reviewers and to everyone who helped improve it. I hope ParB aficionados will enjoy it. www.pnas.org/doi/10.1073/...
- Reposted by Tom McLean#straya Bin Chicken: targeted metagenomic coassembly for the efficient recovery of novel genomes. www.nature.com/articles/s41...
- Reposted by Tom McLeanWhen people celebrate the individual genius of folks in science, they should also mourn the collective loss of genius of folks who were actively discouraged or disadvantaged from a career in science because of the same person(s)
- Reposted by Tom McLeannew preprint from our group & Antoine Hocher: www.biorxiv.org/content/10.1... A fantastic collaboration with Antoine, with Jovana Kaljevic' initiated the collaboration and drives the project.
- Reposted by Tom McLeanVersatile NTP recognition and domain fusions expand the functional repertoire of the ParB-CTPase fold beyond chromosome segregation biorxiv.org/content/10.1101/202…
- Reposted by Tom McLeanEver wondered why some antibiotics are made by Streptomyces on agar plates but not in liquid cultures? Read this work on redox control of antibiotic biosynthesis. Led by katienoble241.bsky.social and Rebecca Devine, and in collaboration with @barriewilks.bsky.social journals.asm.org/doi/10.1128/...
- After 8 wonderful years in Norwich, 3 at UEA and 5 at the JIC it’s time to say goodbye. I will miss each and every amazing person I’ve had the pleasure of working with or meeting here. It truly is a fine city.
- Nice to see our recent work on the fascinating KorAB system be featured on the back of @johninnescentre.bsky.social Advances! Read the full paper here: www.nature.com/articles/s41...
- Check out our lab's new work on a CTP-independent ParABS system. Some fantastic work from Kirill et al!
- Finally published! Many thanks to the reviewers and editor whose comments & suggestions improved this work significantly more than the first preprint version!!! www.pnas.org/doi/10.1073/...
- Have a read of this simply phenomenal work on the weird and wonderful GTAs from star Fellow (and office mate) Emma Banks et al
- 1/ Excited to share our latest work on gene transfer agents (GTAs) in Caulobacter crescentus, led by Emma Banks in collaboration with Pavol Bardy and Mai Nguyen from York!!! See a brief thread below. shorturl.at/o6S1w
- Reposted by Tom McLean"That telomere phages are so prevalent means that they are a selective force, one that we know little about. We now want to understand how the telomere-toxin is secreted and also understand how this ‘telocin’ wheedles its way into unsuspecting bacterial neighbors” www.science.org/doi/10.1126/...
- The paper is now live: academic.oup.com/mbe/article/... Absolutely delighted to play a role in this fascinating story!
- The second great paper concerns the discovery in Eva Top's group that an enigmatic protein encoded by IncP-1beta plasmids modulates the IncP-1 circuitry in E. coli to make the plasmid a burden in this host: Elg et al recently accepted in Molecular Biology and Evolution doi.org/10.1093/molb...
- Reposted by Tom McLeanThe second great paper concerns the discovery in Eva Top's group that an enigmatic protein encoded by IncP-1beta plasmids modulates the IncP-1 circuitry in E. coli to make the plasmid a burden in this host: Elg et al recently accepted in Molecular Biology and Evolution doi.org/10.1093/molb...
- Reposted by Tom McLean#Postdoc position - at the University of Marburg (Germany), funded within our ERC Advanced Grant "C-SWITCH" Focus: CTP-dependent molecular switches as a new principle of cellular regulation in bacteria (including bacteriophages) Apply by 04 May: stellenangebote.uni-marburg.de/jobposting/2...
- Almost 8 years after starting this project finally happy to share the main research from my PhD! Couldn't have done it without the amazing help from @ainsley-beaton.bsky.social! We untangled a fascinating new redox sensor with the most extraordinary growth phenotype (I'm biased) in Streptomyces!
- Very happy to share this work led by @tommclean.bsky.social and @ainsley-beaton.bsky.social. The conserved Streptomyces sensor kinase CutS senses disulphide bond formation outside the cell. If it fails CutS activates CutR which switches on production of foldase HtrA3 www.biorxiv.org/content/10.1...
- Reposted by Tom McLeanFurther insight into how CutRS functions in Streptomyces and some thoughts on how its redox state detection via conserved dual cysteine residues in the extra cellular domain of a sensor kinase may be present in almost all bacteria @tommclean.bsky.social @matthutchings.bsky.social
- Reposted by Tom McLeanVery happy to share this work led by @tommclean.bsky.social and @ainsley-beaton.bsky.social. The conserved Streptomyces sensor kinase CutS senses disulphide bond formation outside the cell. If it fails CutS activates CutR which switches on production of foldase HtrA3 www.biorxiv.org/content/10.1...
- It's done. I've deactivated my twitter account, good riddance.
- Reposted by Tom McLeanRedox control of antibiotic biosynthesis in Streptomyces. Remarkable work led by @katienoble241.bsky.social and Rebecca Devine demonstrates why antibiotic biosynthesis is often switched on in solid cultures and off in liquid. www.biorxiv.org/content/10.1...
- Reposted by Tom McLeanNice blog from the Lister Institute on our recent work on KorAB and long-range gene silencing on multi-drug resistance plasmid!!! @tommclean.bsky.social @johninnescentre.bsky.social lister-institute.org.uk/biology-is-f...
- Reposted by Tom McLeanCool “Behind the paper” article on our recent KorB-KorA sliding clamp-locking clamp story. I remember Chris Thomas and Lewis Bingle (Birmingham) taught me about these proteins when I was an undergrad, and I kept remembering they said the mechanism was not clear!!! www.nature.com/articles/s41...
- Reposted by Tom McLeanOUT NOW: KorB is a DNA clamp which slides along DNA to mediate long-range gene silencing upon interaction with the clamp-locking KorA protein by Tung Le & co @johninnescentre.bsky.social www.nature.com/articles/s41...
- Super excited to share the peer-reviewed version of the KorAB paper. Amazing to work such talented collaborators and thanks to the reviewers for making the work even better! www.nature.com/articles/s41...
- Reposted by Tom McLeanNew paper in PLOS Biol with @graciellehigino.bsky.social et al.: "Postdoctoral scientists are mentors, and it is time to recognize their work". We argue that ensuring that post-docs receive proper credit for ALL their labour would benefit all academia.
- Finally made the pilgrimage to Micropia! Dragged a whole bunch of friends to learn about microbes with me!
- Reposted by Tom McLeanOur review - Antibiotics from rare actinomycetes, beyond the genus Streptomyces - is now online Thanks to coauthors @jonathanparra.bsky.social @ainsleybeaton1 @ryanfseipke @barriewilksjic @matthutchings.bsky.social and editors Toni Gabaldón & Luiz Carvalho for the invitation
- Reposted by Tom McLeanFirst post in this place 💙our last story on the #ParABS system and the unique behaviour of #ParB during the #Bdellovibrio cell cycle is now alive at #PLOSGenetics! We love the revised version (thanks to the 3 super reviewers & great editorial advice). Read it here dx.plos.org/10.1371/jour...
