Luca Giorgetti lab @FMI
We study transcriptional regulation and chromosome folding using an interdisciplinary approach combining wet- and dry-lab methods.
https://giorgettilab.org
@fmiscience.bsky.social
- Reposted by Luca Giorgetti lab @FMI⚠️ The final work of two former PhD students Till @tschwammle.bsky.social and Verena @verenamutzel.bsky.social is out! ➡️⬅️ They dissect how memory can arise from antisense transcription using mathematical modelling 💻, genomics 🧬 and synthetic biology ⚒️! link.springer.com/article/10.1...
- Would anyone out there be able to share some Mus Spretus DNA with us? 🐀🧬 Any tissue of origin, for PCR amplification of selected genomic regions. Many thanks in advance!
- Reposted by Luca Giorgetti lab @FMI📢 Please help spread the word: We’re hiring a Head of Student & Postdoc Affairs to coordinate our international PhD program, provide guidance & career counseling, lead training programs, collaborate on EDI initiatives, and manage alumni relations. Apply at www.fmi.ch/education-ca...
- Reposted by Luca Giorgetti lab @FMIWe just released IRIS (7+yrs project), a tech we believe will transform cell biology by pairing high-resolution cell images with matched #scRNAseq, letting us interpret cellular form by its molecular ground truth. Huge tx to @JohannesBues, @JoernPezoldt, @CamilleLambert et al. shorturl.at/zgY8Z
- And this is a fantastic result too
- Reposted by Luca Giorgetti lab @FMI(23) If you move SRR2 further than ~20kb from the Sox2 promoter, it cannot support cohesin-independent action of the distal enhancer. ~20kb was the cuttof we say at Car2 for cohesin-independent action of enhancers. Makes sense!
- This is a fantastic result!
- (22) Strikingly, you can replace SRR2 with other weak enhancers to restore cohesin-independence at Sox2 > SRR2 is not special > proximal and distal elements likely generally synergize in a cohesin-independent way (thank you @chribue.bsky.social for highlighting the Map4k3 E2 enhancer!)
- Reposted by Luca Giorgetti lab @FMIThe hinge bypass gate paper is finally out! doi.org/10.1038/s414... We show how loop-extruding SMC complexes can maintain DNA entrapment while bypassing obstacles on DNA — including transcription machinery & potentially other SMCs. A lucky convergence of 3 projects lead to the initial discovery!
- Reposted by Luca Giorgetti lab @FMI📣 SAVE THE DATE Next X-inactivation meeting in Sapporo, Japan, 19-23 October 2026. Visit x-inactivation-meeting.org to join our mailing list. 🧬 speakers @dandergassen.bsky.social @marnieblewitt.bsky.social @heard65.bsky.social @crougeulle.bsky.social @sexchrlab.bsky.social @zhouqi1982.bsky.social
- Reposted by Luca Giorgetti lab @FMIOur collab w. V Goel, @nicholas-aboreden.bsky.social , J Jusuf, G Blobel, L Mirny, @irate-physicist.bsky.social out in @natsmb.nature.com www.nature.com/articles/s41... Was co-submitted with @allanaschooley.bsky.social @jobdekker.bsky.social whose paper should also come out soon Brief thread 👇
- been lurking here a while but time to spend less time in the bad place Here's my latest work in collab w/ Viraat Goel, @andersshansen.bsky.social, Leonid Mirny, Nick Aboreden, Gerd Blobel et al. Dynamics of microcompartment formation at the mitosis-to-G1 transition www.biorxiv.org/content/10.1...
- Reposted by Luca Giorgetti lab @FMIWhat is a promoter? And how does it work? We very happy to share our latest work trying to understand enhancer-promoter compatibility. I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters www.biorxiv.org/content/10.1...
- Reposted by Luca Giorgetti lab @FMIThe TArgeted Cohesin Loader (TACL) paper was just published. Happy that we were able to contribute to this really exciting project! If you want to learn how targeting cohesin to defined loci in the genome affects the local chromatin environment and transcription, look no further! rdcu.be/eLiT5
- Reposted by Luca Giorgetti lab @FMIWe are recuiting two new Associate Professors here in Oxford Biochemistry. Come join us! Reach out to me if you have any questions. Please repost! tinyurl.com/mr3m7bd3
- Reposted by Luca Giorgetti lab @FMICome join us in Geneva for everything epigenetics and gene regulation. It will be a great meeting! Please repost! www.keystonesymposia.org/conferences/...
- Reposted by Luca Giorgetti lab @FMI⚠️ Paper alert: Using a novel CRISPR screening approach, we mapped the entire regulatory network controlling Xist—key for X-chromosome inactivation. 👉 We discover how sex and development signals are decoded at a single gene locus. www.nature.com/articles/s41... 👇 Bluetorial
- Reposted by Luca Giorgetti lab @FMI[Not loaded yet]
- Reposted by Luca Giorgetti lab @FMIThe latest work from ours and @vram142.bsky.social lab is out! True teamwork to visualize nascent chromatin with strand resolution, using a fully reconstituted system. Very proud of superstar-PhD student Bruna, and @palindromephd.bsky.social. Learning so much from Vijay’s amazing technologies! RT
- Some (+)ve news to lighten another heavy weekend: our latest preprint (c/o Mattiroli + Ramani labs) is up! www.biorxiv.org/content/10.1... A tour-de-force by 1st authors Bruna Eckhardt & @palindromephd.bsky.social, focusing on chromatin replication. RTs welcome; tweetorial in 3,2...(1/n)
- Reposted by Luca Giorgetti lab @FMI1/ 🧵 I am excited to share a new preprint from our group: Single-cell Dam&T-seq during mouse corticogenesis reveals how genome–lamina interactions regulate long neuronal genes. doi.org/10.1101/2025... #Neurodevelopment #Epigenetics @hubrechtinstitute.bsky.social @oncodeinstitute.bsky.social
- Really excited to share our latest work led by @mattiaubertini.bsky.social and @nesslfy.bsky.social: we report that cohesin loop extrusion creates rare but long-lived encounters between genomic sequences which underlie efficient enhancer-promoter communication. www.biorxiv.org/content/10.1... A🧵👇
- 2/ We started by asking: How often, and for how long, do two genomic sequences meet in the cell nucleus within an arbitrary encounter radius? And how does this depend on the loop extrusion activity of cohesin?
- 3/ Previously, @andersshansen.bsky.social's lab and us (@piamach.bsky.social @pavelkos.bsky.social ) showed that CTCF loops last ~10-30 minutes. But how about any other encounters, i.e. those that are not stabilized by CTCF?
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View full thread19/ Read about this and much more in our preprint: www.biorxiv.org/content/10.1... Big shoutout to @laureplantard.bsky.social and Laurent Gelman from the @fmiscience.bsky.social facility for imaging & advanced microscopy!
- Reposted by Luca Giorgetti lab @FMINew work from my lab with the Brown lab on how genome folding changes during motor neuron maturation, and how this process goes awry in ALS motor neurons. Led by Dr. Ozgun Uyan. www.biorxiv.org/content/10.1...
- Reposted by Luca Giorgetti lab @FMIAnother FMI Annual Meeting is in the books! After a night of follies and plenty of dancing, today brought the announcement of our internal prize winners, more inspiring talks, and a vibrant poster session. Already looking forward to next year! 🙌
- Reposted by Luca Giorgetti lab @FMI📣The Turco group is seeking a computational biologist to analyze large omics datasets. Work on cutting-edge 3D organoid models of the human placenta and be embedded in our Computational Biology Platform (part of @sib.swiss). Apply here: www.fmi.ch/education-ca...
- Reposted by Luca Giorgetti lab @FMII am excited to announce that we are looking for a Lab Manager @fmiscience.bsky.social in Basel. If you want to be part of a growing team investigating development and tissue formation, and are enthusiastic about helping set up a new lab, please check out the role: www.fmi.ch/education-ca...
- 🚨 Job Alert: we seek a Lab Manager for @juliabatki.bsky.social's new group (start Mar 2026), which will study cell fate regulation during organ development. Join the Batki's group to drive experiments & lab operations! Apply at: www.fmi.ch/education-ca...
- Become our colleague at @fmiscience.bsky.social! The FMI is a very special place to start your lab: a vibrant international institute with world-class research groups and facilities, core funding, fantastic trainees, great colleagues😅, and a collegial culture. Get in touch if you're interested!
- 🚨 We're hiring, please share! The FMI seeks a tenure-track Group Leader (Assistant Prof) in Structural Biology 🔬 Innovative scientists in genome regulation, RNA metabolism, or protein homeostasis—especially using cutting-edge approaches—apply now at www.fmi.ch/education-ca...
- Reposted by Luca Giorgetti lab @FMIThis is not a HiC map! Ever wondered if multiple enhancers get activated simultaneously? We measured chromatin accessibility on thousands of molecules by nanopore to create genome-wide co-accessibility maps. Proud of @mathias-boulanger.bsky.social @kasitc.bsky.social Biology in the thread👇
- Activity of most genes is controlled by multiple enhancers, but is there activation coordinated? We leveraged Nanopore to identify a specific set of elements that are simultaneously accessible on the same DNA molecules and are coordinated in their activation. www.biorxiv.org/content/10.1...
- Reposted by Luca Giorgetti lab @FMINew preprint with @gfudenberg.bsky.social We find the rate of cohesin loop extrusion in cells is set by NIPBL dosage and tunes many aspects of chromosome folding. This provides a molecular basis for NIPBL haploinsufficiency in humans. 🧵👇 www.biorxiv.org/content/10.1...
- Reposted by Luca Giorgetti lab @FMIWe are hiring a scientist to lead our 'Build' team at the Seattle Hub for Synthetic Biology. @alleninstitute.org Job posting below: alleninstitute.org/careers/jobs... Please reach out if you have any questions!
- Reposted by Luca Giorgetti lab @FMIExcited to share the latest work from the lab led by @eharo84.bsky.social, in which we have used synthetic biology to explore the mechanisms by which different types of long-range enhancers ensure robust and precise developmental gene expression www.biorxiv.org/content/10.1...
- Reposted by Luca Giorgetti lab @FMIWe found a new asymmetry in the large-scale chromosome structure: sister chromatids are systematically shifted by hundreds of kb in the 5′→3′ direction of their inherited strands! The work was led by Flavia Corsi, in close collaboration with the Daniel Gerlich lab. www.biorxiv.org/content/10.1... 1/
- Reposted by Luca Giorgetti lab @FMIOur paper describing the Range Extender element which is required and sufficient for long-range enhancer activation at the Shh locus is now available at @nature.com. Congrats to @gracebower.bsky.social who led the study. Below is a brief summary of the main findings www.nature.com/articles/s41... 1/
- Reposted by Luca Giorgetti lab @FMIFinally out! 🥳 Our paper showing how a transposable element (TE) insertion can cause developmental phenotypes is now published @natgenet.nature.com 🧬🦠🐁 Below is a brief description of the major findings. Check the full version of the paper for more details: www.nature.com/articles/s41588-025-02248-5
- Reposted by Luca Giorgetti lab @FMIOur work bridging enhancer-promoter proximity to phenotypic outcomes in vivo is out! Shout out to @olimpiabompadre.bsky.social, to Marie Kmita's lab, and to all the co-authors. www.nature.com/articles/s41...
- We are looking for a research assistant to join our lab and support ongoing projects with genome engineering in mouse embryonic stem cells! Please get in touch if you are interested. www.fmi.ch/education-ca...
- Reposted by Luca Giorgetti lab @FMIHow to find Evolutionary Conserved Enhancers in 2025? 🐣-🐭 Check out our paper - fresh off the press!!! We find widespread functional conservation of enhancers in absence of sequence homology Including: a bioinformatic tool to map sequence-diverged enhancers! rdcu.be/enVDN github.com/tobiaszehnde...
- Reposted by Luca Giorgetti lab @FMI(1/n) Thread @matteomazzocca.bsky.social @domenicnarducci.bsky.social Simon Grosse-Holz @jessematthias.bsky.social preprint Q: how does chromatin move? Using MINFLUX, SPT & SRLCI, we track chromatin dynamics across 7 orders of magnitude in time to provide answers www.biorxiv.org/content/10.1...
- Reposted by Luca Giorgetti lab @FMI1/ Super excited to share my first preprint from @andersshansen.bsky.social Lab! We used MINFLUX to track chromatin (H2B-Halo and Fbn2 locus) at an unprecedented 200 μs, then combined it with SPT to span μs-minutes (H2B) or SPT & Super-Res Live-Cell Imaging (SRLCI) to span μs-hours (Fbn2)
- Reposted by Luca Giorgetti lab @FMICheck out our latest work on the evolution of animal genome regulation out today in @nature.com. Nicely summarized below by @ianakim.bsky.social. www.nature.com/articles/s41... This is a major output from our ERC-StG project Evocellmap @erc.europa.eu at @crg.eu
- I’m very excited to share our work on the early evolution of animal regulatory genome architecture - the main project of my postdoc, carried out across two wonderful and inspirational labs of @arnausebe.bsky.social and @mamartirenom.bsky.social. www.nature.com/articles/s41...
- Reposted by Luca Giorgetti lab @FMII’m very excited to share our work on the early evolution of animal regulatory genome architecture - the main project of my postdoc, carried out across two wonderful and inspirational labs of @arnausebe.bsky.social and @mamartirenom.bsky.social. www.nature.com/articles/s41...
- Reposted by Luca Giorgetti lab @FMIOur latest work now online in Cell: Rewriting regulatory DNA to dissect and reprogram gene expression Our new method (Variant-EFFECTS) uses high-throughput prime editing + flow sorting + sequencing to precisely measure effects of noncoding variants on gene expression Thread 👇
- I am elated to share that our manuscript describing Variant-EFFECTS, a high-throughput technology we developed to precisely and quantitatively measure the effects of CRISPR-mediated edits on gene expression, is now published at @cellpress.bsky.social: authors.elsevier.com/c/1kxgiL7PXu...
- Reposted by Luca Giorgetti lab @FMI[Not loaded yet]
- Reposted by Luca Giorgetti lab @FMIThe importance of moving away from bulk! Occupancy of Pol II at promoters is dramatically different between fly and mouse cells! When looking single molecule! Proud of the team! @kasitc.bsky.social @molinalab.bsky.social doi.org/10.1038/s443...
- Celebrating 10 years of our lab with a new preprint: www.biorxiv.org/content/10.1... How does enhancer location within a TAD control transcriptional bursts from a cognate promoter? Experiments by Jana Tünnermann and modelling by Gregory Roth
- (2/n) We modified our enhancer mobilization assay (www.nature.com/articles/s41...) to enable MS2-mediated visualization of active transcription sites when the SCR enhancer is hopped and reinserted into different random positions around an ectopic copy of the Sox2 promoter inside a ‘neutral’ TAD.
- (3/n) We show that enhancer position controls the promoter burst frequency, but not burst duration and size
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View full thread(10/n) We would love to receive feedback and comments, and looking forward to presenting this work next Monday at the Keystone Symposium on Interplay of Chromatin Architecture and Transcription Regulation www.keystonesymposia.org/conferences/...
