Christopher Whitford
Engineering streptomycetes at the Novo Nordisk Foundation Center for Biosustainability
NNF Postdoctoral Fellow
Scholar: tinyurl.com/yw82k2cr
- Reposted by Christopher Whitford#OpenCloning is a an Open Source alternative to SnapGene/Benchling that supports automation and integration with other software ✅ Free 🔓 Open Source 🧬 More cloning methods than SnapGene 🤖 Can be automated with python 👨🔬 Built by a researcher — for researchers! 👉 Check it out at opencloning.org
- Check out this new amazing preprint by David, @tuesparholt.bsky.social , @thombooth.bsky.social, and @tilmweber.bsky.social!
- Bacterial telomeres are common, just not so much in RefSeq 'complete' genomes. But they can be added by the new tool David Faurdal wrote. I am thrilled to see this out as a preprint here: www.biorxiv.org/content/10.1... @tilmweber.bsky.social @thombooth.bsky.social
- New paper alert!! Led by Lucas, we automated all the design steps for genome engineering experiments in streptomycetes. If you are using our Streptomyces CRISPR toolbox, you can now design hundreds of experiments in a matter of minutes. @tilmweber.bsky.social @kblin.bsky.social ....
- All design parameters are automatically tracked, making it very easy to reproduce & compare experimental outcomes. All workflows further come with html protocols, so it's super easy to just get started in the lab.
- Interested in engineering streptomycetes? Struggling with existing genome engineering tools? Then check out our newest paper on CASCADE-Cas3 based genome engineering in streptomycetes! Some highlights below:
- 1/8 We found that type I CRISPR systems (like CASCADE-Cas3) are much more widespread in streptomycetes than type II CRISPR systems (e.g. Cas9). CASCADE-Cas3 also has a TA rich PAM, which translates to way fewer potential off-target sites.
- 2/8 The minimal type I-C CASCADE-Cas3 is fascinating. A complex consisting of Cas5, Cas7, and Cas8 binds the target site and recruits the processive nuclease Cas3, which generates recombinogenic overhangs! Sounds great for homologous recombination!
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View full thread8/8 Finally, a big thank you to all co-authors - Peter, David, Renata, Tetiana, and @tilmweber.bsky.social ! This has been a fun collaboration!
- Interested in conducting your research in a great and innovative environment? Interested in doing so in a politically stable country (as opposed to e.g. the US of A)? Denmark is actively recruiting researchers from abroad. Can only recommend! research.state-of-denmark.com?utm_campaign...
- Reposted by Christopher WhitfordIt’s been a tough few weeks. My 10yo daughter was diagnosed with a very rare, aggressive cancer called interdigitating dendritic cell sarcoma (IDCS). I’m reaching out to identify clinicians/patients who have encountered pediatric IDCS or other (non-LCH) dendritic or histiocytic sarcomas cases.
- Reposted by Christopher WhitfordIf you’re at a European institution and are looking to poach American scientists, this would be a very good week to do it
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