Cool work from Heath Murray's lab where they use Cas9 nickase to study how ssDNA breaks are repaired in Bacillus subtilis
#MicroSky www.nature.com/articles/s41...Rescuing the bacterial replisome at a nick requires recombinational repair and helicase reloading - Nature Communications
DNA damage can lead to cell death. Here, the authors show that a simple cut on either strand of DNA can inactivate bacterial chromosome replication. Surprisingly, only a core set of recombination prot...
