Roman Doll
PhD Student in Medical Sciences at the University of Oxford.
If it has a genome, we can do genome editing on it, right? 🧬
- Reposted by Roman DollMake your gene knockouts more efficient with multiplexed Cas12a sgRNAs. Our new paper is out now, with tools available from @addgene.bsky.social , www.plasmids.eu and @vdrc-flies.bsky.social. www.nature.com/articles/s41... #CRISPR #geneediting #Drosophila 🧪🧬✂️🔬🪰 Summary 🧵 below.
- Excited to share that a part of my PhD work is online now on BioRxiv! www.biorxiv.org/content/10.6... We developed a genome editing approach to target the JAK2 V617F mutation and demonstrate its potential to alleviate MPN hallmarks in primary patient cells. Thread 👇
- JAK2 V617F is a common haematological driver mutation, but JAK2 inhibitors fail to clear mutant cells in most patients with myeloproliferative neoplasms (MPNs).
- Check out the latest work from the @davieslab.bsky.social led by Hangpeng!
- Our latest paper has just been published in Cell! doi.org/10.1016/j.ce... We developed a new method called MCC ultra, which allows 3D chromatin structure to be visualised with a 1 base pair pixel size.
- Reposted by Roman Doll[Not loaded yet]
- Reposted by Roman DollOur new preprint is out - please share! We asked: How can we make CRISPR knockouts in vivo more efficient? And set up an assay that enables detection of CRISPR cutting with 100s-1000s of sgRNAs over entire chromosome arms in living animals. Grab a ☕ and let’s dive in. 🧵🧪 1/n #CRISPR #geneediting
