New Article! A tripartite cell-free translation system to study mammalian translation
A tripartite cell-free translation system to study mammalian translation
Nature Protocols, Published online: 16 April 2025; doi:10.1038/s41596-025-01155-7This protocol presents a tripartite mammalian cell-free translation system, comprising cytoplasmic and ribosome fractions combined with endogenous or synthetic mRNAs, permitting flexible reconstitution and careful dissection of the mechanisms of translation.
