Somehow missed this one. It appears to be yet another important study from the Kaeser group: "GRAB signals aren't pure; there is always cross-talk, and sometimes the cross-talks become problematic. And no, HEK cell measurements don't immediately translate in vivo..."
www.biorxiv.org/content/10.1...
Innervation density governs crosstalk of GPCR-based norepinephrine and dopamine sensors
GPCR-based fluorescent sensors are widely used to correlate neuromodulatory signaling with brain function. While experiments in transfected cells often reveal selectivity for individual neurotransmitt...
