Despite daily disruptions, scientists try to use logic and creativity to help fellow humans.
Thus I would like to report our latest work describing microbial-independent deep assembly and screening, a method that goes from primers to mammalian cell assays in 1 day.
www.biorxiv.org/content/10.1...
Rapid optimization of protein function in mammalian cells via microbe-independent deep assembly and screening
Random mutagenesis and deep mutational scanning (DMS) are widely used to optimize proteins by oversampling large libraries in microbial cells, selecting cells expressing favorable variants, and sequen...
