GenomiqueENS
Genomics core facility of IBENS @ibensens.bsky.social - Ecole Normale Supérieure @normalesup.bsky.social - @psl-univ.bsky.social
#Nanopore #transcriptomics #SingleCellRNAseq #eucaryotes #low-input
website: genomique.biologie.ens.fr
- Our expertise in single-cell transcriptomics in organisms with fragmentary annotation is illustrated by this new study in chick, revealing new roles for Cdkn1 in the neurogenic transition.
- Are you working with exotic/non-model species? 🐸🐠🐓🦋🐝 Your annotation needs improvement to ensure RNA-seq or single-cell RNA-seq counts are correctly handled (for exemple the 3’UTRs)?
- 🧬Searching for a Nanopore RNA-seq protocol that generates longer reads than the manufacturer’s protocol, and applicable for low-input material ? 🤔
- 🎉 We are thrilled to announce that our protocol “Library construction for ONT long read cDNA”, developed at our GenomiqueENS core facility, is available on protocol.io. dx.doi.org/10.17504/protocols.io.4r3l21oepg1y/v1
- This protocol, combining SMART-Seq® mRNA kit (Takara Bio Europe) and EXP-PCA001 and SQK-PCB114 kits (@nanoporetech.com) allows full-length transcripts to be obtained.
- 🎉 We are very proud of Ali Hamraoui who defend his PhD thesis on bioinformatics approaches to study isoforms with single-cell long-read technologies.
- 🚀 Our new preprint is now live on bioRxiv! “A systematic benchmark of bioinformatics methods for single-cell and spatial RNA-seq Nanopore long-read data” 🔗 Read here: www.biorxiv.org/content/10.1... 🔬 About the study We present the first comprehensive benchmark of bioinformatics tools 👇
- 🎉 Our core facility has just renewed its certifications ISO 9001 and NFX-50-900 ! These certifications are particularly important with regards to our users, but also as we are member of @fr-genomics.bsky.social.
- For our 1st post on Bluesky, we are excited to announce the publication of our new #bioinformatics tool #Asarusim for #SingleCellRNAseq coupled with @nanoporetech.com sequencing ! 🎉 🎊 👉 article : academic.oup.com/bioinformati... 👉 GitHub : github.com/GenomiqueENS... cc @fr-genomics.bsky.social