New paper introduces a virus replicon–based genome-wide
#CRISPR KO screening platform that identifies
#HostFactors essential for replication of diverse RNA viruses, from dengue to chikungunya and Ebola.
#Virology
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doi.org/10.1038/s41467-025-…
👤 EVBC member: Jens H. Kuhn
Replicon-based genome-wide CRISPR knockout screening for the identification of host factors involved in viral replication - Nature Communications
Cheng et al., use non-infectious stable viral replicon cell lines in genome-wide CRISPR KO screens across diverse viral pathogens to identify host factors specifically involved in the intracellular stages of viral replication.
