- Comparing multiphoton (2p/3p) imaging systems is often done poorly. labrigger.com/blog/2026/01... E.g., PSFs can be measured differently by different labs, staining quality is by far the biggest determinate on how multiphoton images will look at depth, and so forth. (1/3)Jan 21, 2026 20:55
- The key issue is that the authors who try to make comparisons don't have access to all of the systems and can't make apples-to-apples comparisons. But they present an illusion of that, using anecdotes in place of rigorous comparisons. Which makes the comparisons wrong. (2/3)
- Fortunately, there's a resource to improve benchmarking of multiphoton systems. So things can get better. Here's the paper www.nature.com/articles/s41... And here's the free PMC version PDF (pmc.ncbi.nlm.nih.gov/articles/PMC...) (3/3)
