Wei-Chung Allen Lee
circuit motifs of action selection, execution, & refinement | functional connectomics | assoc prof | lee.hms.harvard.edu
- Reposted by Wei-Chung Allen LeeOur new paper showcasing molecular connectomics with pan-expansion microscopy is out in @natbiotech.nature.com! www.nature.com/articles/s41... This wonderful collaboration with @bewersdorflab.bsky.social was led by Ons M'Saad (now founder/CEO of Panluminate) and @allisonphysics.bsky.social. (1/5)
- Reposted by Wei-Chung Allen LeeHappy to share the first paper from my journey at @psich.bsky.social towards X-ray connectomics, now out in @natmethods.nature.com: www.nature.com/articles/s41...
- Meet Wangchu and connectomics in the spinal cord this afternoon at LBP077.04 / LBP036 #SFN25
- Stop by Wangchu's poster Tuesday (11/18) afternoon to learn about circuit motifs supporting touch processing in the spinal cord.
- Reposted by Wei-Chung Allen LeeExciting news for #drosophila #connectomics and #neuroscience enthusiasts: the Drosophila male central nervous system connectome is now live for exploration. Find out more at the landing page hosted by our Janelia FlyEM collaborators www.janelia.org/project-team....
- Reposted by Wei-Chung Allen LeeIt was a good experience to step back and briefly take stock of the amazing progress in connectomics since I started working on this stuff (20 years ago!) thanks as well to @natrevneuro.nature.com for the constructive editorial interactions.
- Synaptic connectomics: status and prospects — a Comment article by Davi D. Bock @dddavi.bsky.social #neuroscience #neuroskyence www.nature.com/articles/s41...
- Reposted by Wei-Chung Allen LeeX-ray nanotomography can be your new friend for neuronal imaging. In our new #preprint (tinyurl.com/nanoXneuro) we present advances that push the spatial resolution limit for X-ray holographic nanotomography (XNH). We can now resolve synapses. 🧵
- Reposted by Wei-Chung Allen LeeMy PhD project is out as a preprint! We combined 2P and synchrotron X-ray to understand mouse olfactory bulb circuits, linking physiology to structure in 3 animals! doi.org/10.1101/2025... 🙌 @carlesbosch.bsky.social, @apacureanu.bsky.social, @andreas-t-schaefer.bsky.social, @esrf.fr, @crick.ac.uk
- Couldn't agree more. There’s still so much to be uncovered in the dataset. One thing that wasn’t emphasized before is how it enables access to specific circuit motifs down to identified cells. 12 examples are highlighted in the preprint, but again just the tip of the iceberg.
- How is the nervous system organized to coordinate behavior? To approach this massive question, a team led by @asbates.bsky.social, @jasper-tms.bsky.social, @mindyisminsu.bsky.social, & Helen Yang present the BANC: a Brain and Nerve Cord connectome. Preprint: doi.org/10.1101/2025... 🧪#Neuroskyence
- Public access to the first fly connectome that spans the whole CNS - BANC!: codex.flywire.ai?dataset=banc Different from prior connectomes - it is brain + cord (think spinal cord) We use it to ‘embody’ the system and find it resembles ‘subsumption architecture’ doi.org/10.1101/2025...
- The BANC is the first connectome that explicitly links the brain to the nerve cord, & through it, to the body. It offers a new “embodied” perspective for connectomes, one that changes how we think about neural networks for control, behavior, & even artificial intelligence. bsky.app/profile/mott...
- I think the "embodiment" of this connectome is going to be such a hit: For instance take the interactive "body part" maps on Codex, where you can simply click on your favorite external or visceral part and it will show you all of the neurons associated it! codex.flywire.ai/app/body_par...
- The dataset includes ~160,000 cells, segmented nuclei and mitochondria, synapses and neurotransmitter predictions, and annotations linking the CNS to peripheral sensory, motor, and visceral systems. Info: banc.community Data: codex.flywire.ai?dataset=banc Viewer: ng.banc.community/view
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View full threadKey annotations from: Tony Azevedo, @tuthill.bsky.social , @zandawala.bsky.social, @hokuba.bsky.social, Steffi Hampel, @andrew-seeds.bsky.social, Kathi Eichler @jefferis.bsky.social, Michael Pankratz, @fleyes.bsky.social, & Marie Suver. Renderings by @amysterling.bsky.social and Arie Matsliah
- With @megyounger.bsky.social's lab, et al., we present the first connectomics work in the disease-vector mosquito Aedes aegypti, revealing how its brain is wired to detect host cues. Preprint: doi.org/10.1101/2025... #Neuroscience #Connectomics #vEM #VectorBiology 🧪
- New preprint from @darbly.bsky.social @briandepasquale.bsky.social and my labs! If you love (or hate) mosquitoes, have a look: doi.org/10.1101/2025... We used TEM to describe the circuitry used for CO2 detection by mosquitoes. As usual with mosquitoes, nothing is as expected! 🌬️🦟
- Female Ae. aegypti mosquitoes track humans by sensing host cues including CO₂ humans release. CO₂ activates mosquitoes and sensitizes them to other cues like odor and heat, but how is this implemented in its brain?
- We used large-scale electron microscopy to reconstruct the wiring and connectivity of the CO₂-responsive microcircuit (Glomerulus 1) and its neighbors in a mosquito brain.
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View full threadThanks to those that supported the work including: @wellcometrust.bsky.social, @klingensteinorg.bsky.social, @simonsfoundation.org, @sloanfoundation.bsky.social, Searle Scholars Program, the Smith Family Foundation, and the Pew Charitable Trusts.
- Reposted by Wei-Chung Allen LeeNew preprint: "The geometry of the neural state space of decisions", work by Mauro Monsalve-Mercado, buff.ly/42wVHD5. Surprising results & predictions! (Thread) We analyze neuropixel population recordings in macaque area LIP during a reaction time, random-dot motion 1/
- Reposted by Wei-Chung Allen Lee(1/30) New preprint! "Symmetries and continuous attractors in disordered neural circuits" with Larry Abbott and Haim Sompolinsky bioRxiv: www.biorxiv.org/content/10.1...
- Thanks Albert, @jefferis.bsky.social, & Joergen for a wonderful visit. Future of connectomics is bright at the @mrclmb.bsky.social.
- Begets equity abcnews.go.com/US/massachus...
- Reposted by Wei-Chung Allen Lee[This post could not be retrieved]
- Let’s go!!
- Reposted by Wei-Chung Allen Lee[This post could not be retrieved]
- Reposted by Wei-Chung Allen LeeExcited about our minisymposium at @SfNtweets! Join us Wednesday afternoon to hear about the functional organization of lateral septum - from molecules to circuits and computations! Session details here: abstractsonline.com/pp8/#!/20433... #sfn2024 #neuroscience
- Amazed seeing @bdsc.bsky.social in person the other week. Remarkably, they maintain >100,000 fly lines - with geo-redundancy - and provide them to users around the world! A vital scientific resource.
- Reposted by Wei-Chung Allen LeeCome see two of our lab projects tomorrow morning at SfN. Fabricio will present behavior, closed loop opto, and EMG in a really cool mouse jumping behavior: E32 Praising (Jerry) will present Neuropixel recordings from the spinal cords of awake behaving mice performing simple reflexes: G12
- Reposted by Wei-Chung Allen LeeUCSF Francisco researcher Corey Harwell talks about his own biography first Louisville. He is showing data about the town, maps of ethnicities. 🧠🧪 Took part in MARC program at Tennessee State = Maximizing Access to Research Careers. Also: summer research program stints.
- Reposted by Wei-Chung Allen LeeOur fly brain connectome papers are now live (www.nature.com/immersive/d4...), and is getting traction (www.bbc.co.uk/news/article...). With ~139k neurons, >8k cell types, synapses annotated, transmitters predicted, ~134 evo-devo units denoted, so much is now possible in the fly.
- Reposted by Wei-Chung Allen LeeThe fly now has a full #connectome, in its most basic form a directed + weighted graph. But what are its connection ‘signs’, excitatory/inhibitory/modulatory? Our new, now published work moves towards this answer, predicting #neurotransmitters from EM! 1/n www.cell.com/cell/fulltex...
- Reposted by Wei-Chung Allen LeeHard to overstate how pleased I am that the FlyWire consortium converted our FAFB ("Full Adult Fly Brain") EM volume into a connectome, and that the resulting science has been (and will be) so impactful. Thanks to everyone involved. #neuroscience www.nature.com/immersive/d4...
- The essential parts list.
- And our contribution to FlyWire, cell typing the connectome with @jefferis.bsky.social and colleagues, is here: www.nature.com/articles/s41...
- Excited to see this out: rdcu.be/dzfgM Congratulations Aaron Kuan (now with a new lab at Yale), Giulio Bondanelli, and all who made this years-long work on neural circuits supporting decision-making possible! #neuroscience
- Beyond the preprint (summarized elsewhere): x.com/darbly/statu... new analyses and predictions, including that opponent inhibition between neurons with activity at different times contributes to activity sequences.
- Looking for a 2024 calendar? catalog.ninds.nih.gov/publications...
- Reposted by Wei-Chung Allen Lee3D-Imaging of synapses in neuronal tissues with synchrotron X-ray ptychography https://www.biorxiv.org/content/10.1101/2023.11.16.567403v1
- Reposted by Wei-Chung Allen LeeGood morning #SfN23 people! Our lab has 3 posters this morning (Sunday Nov 12) and 2 tomorrow morning (Monday Nov 13). We'd love to see you and share our latest on fly vision, circuits, and behavior.
- A neat technical aspect of this study is that we went back to the same TEM grids imaged a decade earlier and re-imaged them at sub-nanometer resolution for additional information. A distinct advantage of sample-preserving EM and as Sydney Brenner noted data that is complete, accurate, and permanent.
- Another spectacular paper about cilia and their structural and positional diversity in the brain. Wonder why oligodendrocytes and microglia don't have 'em but astrocytes do. From Carolyn Ott at Janelia 🧪 🧠🟦 www.biorxiv.org/content/10.1...
- Congratulations to Annie, Qiyu and team! Beautiful end organ reconstructions provide a unified model of mechanosensory transduction. www.cell.com/neuron/fullt...
- Remarkable in vivo recordings from Pacinians
- Also see this complementary work: www.biorxiv.org/content/10.1...
- Reposted by Wei-Chung Allen LeeCerebellar circuits for disinhibition and synchronous inhibition https://www.biorxiv.org/content/10.1101/2023.09.15.557934v1
- Reposted by Wei-Chung Allen LeeSynaptic and peptidergic connectomes of the Drosophila circadian clock https://www.biorxiv.org/content/10.1101/2023.09.11.557222v1
- Reposted by Wei-Chung Allen LeeNetworks of descending neurons transform command-like signals into population-based behavioral control https://www.biorxiv.org/content/10.1101/2023.09.11.557103v1
