Lorenzo Olivi
In love with single molecules 🔬 moving inside tiny things 🦠 and big dumb monsters 🦖 wreaking havoc on the screen 🎬
Postdoctoral researcher @ Laboratory of Microbiology (WUR)
Also on Letterboxd -> boxd.it/5Z17P
- 🧵 Our study on the elusive titration control mechanism of the replication initiator DnaA in Escherichia coli is now out on @natcomms.nature.com! @stephkoe.bsky.social @hohlbeinlab.bsky.social @nicoc-micsynmet.bsky.social @ettema.bsky.social @mib-wur.bsky.social doi.org/10.1038/s414...
- 2 In E. coli, DNA replication starts when DnaA binds to the origin of replication oriC and unwinds it, allowing for the rest of the DNA polymerase to assemble. This initiation step must be finely regulated to ensure that DNA replication happens timely and only once per cell cycle.
- 3 DnaA binds DNA in specific 9 bp motifs called DnaA boxes. These boxes can have high or low affinity, depending on how well they match a specific consensus sequence. There are hundreds of high affinity boxes spread across the chromosome of E. coli. However, oriC mostly contains low-affinity motifs.
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View full thread12 Bonus! DnaA is also a transcription factor, so together with @baybioms.bsky.social we also investigated how changes in its regulation alter E. coli proteome! Surprisingly, impacting titration or switch alone seems to have no impact on the genes known to be regulated by DnaA...
- Definitely an experience to say the least, but stoked to see it in its final form! Can't wait to share more
- Heroic effort by @loreoliv.bsky.social to wrap that one up! Reviewers' comments: similar number of words as in the manuscript... More soon.
- This is the science that really matters
- 1/n Some time ago my colleague, excellent cook, and friend Ivan told me: "Cacio e pepe is the recipe that I screw up more often. Let's make a project studying systematically the physics of that sauce". Prepare to get cheesy, I'm glad to share the Cacio e paper preprint: arxiv.org/abs/2501.00536
- Reposted by Lorenzo OliviThe tech bro fascination with eugenics is so on brand. The idea that you could make accurate, actionable predictions from individual genotypes (with all their complex, non-linear interactions) from averaged, linearly modeled population-level genomic statistics is just another big data fantasy
- Reposted by Lorenzo OliviIn the spirit of which, here some new filter wheels designed by Ian Kercher with more info available here: www.printables.com/model/110377... #OpenMicroscopy
- Reposted by Lorenzo OliviWe strongly suggest that academic publishers and other platforms that host research rapidly implement a Share to Bluesky button for their articles. Here's how: docs.bsky.app/docs/advance... #AcademicSky #HigherEd #Altmetrics
- The best resource for #microscopy is among us on BlueSky! Savior of my #sptPALM designs
- Starting to think I should use our dog Ombra as a metaphor to explain students how fluorescent proteins allow you to tell apart things of interest from the background
