Structura Biotechnology
Algorithms, Software and Systems for Cryo-EM. Makers of CryoSPARC.
- Very excited for this in-person #CryoSPARC workshop in partnership with @sickkidsto.bsky.social and the Canadian #CryoEM Nexus! Register today!
- @structurabio.bsky.social and @sickkidsto.bsky.social CryoSPARC workshop in Toronto! April 30/May 1st 2026. Part of the Canadian Cryo-EM Nexus. Please register here: sickkids.cventevents.com/event/a0aebb...
- 🚀 CryoSPARC v5.0 BETA is here! We’re excited to deploy another major #CryoSPARC release to help enable and accelerate #cryoEM data analysis. v5 has a redesigned underlying software system and many new features - highlights in thread! Full changelog: cryosparc.com/updates/v5.0.0
- 2/ Masking during 3D refinement has been significantly reworked. Dynamic mask generation now adapts automatically as resolution changes, reducing the likelihood of overfitting or overestimated resolution, without users needing to modify parameters.
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- Reposted by Structura BiotechnologyExciting collab with Paul Palme, Peter Imming, & Adrian Richter! Guided by @courbongautier.bsky.social's structure of myco ATP synthase bound to a squaramide, they have made SQAs that are less toxic, more potent, more stable than AstraZeneca's SQ31f #MedChem #TB #NTM pubs.acs.org/doi/full/10....
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- 🚀 Hiring: Cryo-EM Application Scientist, Marketing! Develop and deliver high-impact scientific marketing content, illustrate the value of new #CryoSPARC features and #cryoEM advances, and help scientists understand how our tools can help solve their problems. structura.bio/careers/appl...
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- Reposted by Structura BiotechnologyNew lab preprint! @zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes 🧵 tinyurl.com/ukny4ptz
- On a gruelling hunt for rare associated molecules in your #cryoEM particle stack and not sure where to look? Discover practical tips and tricks in our new case study using #CryoSPARC v4.7.1 where we find and refine a low-population interaction partner! guide.cryosparc.com/processing-d...
- 🚀 Introducing fully automated data processing for repeat-target #cryoEM. Using new tools in #CryoSPARC, it is now possible to obtain resolutions & map quality equal to or better than manual processing, with zero user intervention. Preprint: www.biorxiv.org/content/10.1...
- The automation strategy we have developed can be set up once for any specific target class (e.g. active state GPCRs with Nb35) and reused to process multiple datasets fully hands-off. It makes use of minimal prior knowledge through a low-res (15Å) 3D reference map.
- Reposted by Structura BiotechnologyEnd-to-end automation of repeat-target cryo-EM structure determination in CryoSPARC biorxiv.org/content/10.1101/202…
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- Reposted by Structura Biotechnology1/14 Happy (but mostly relieved) to share my dream project with @boudkerlab.bsky.social, now published in @natsmb.nature.com! We used evolution, protein engineering & cryoEM to uncover how ion coupling in glutamate transporters works, and how it evolved.🧵 Free article: go.nature.com/4oRUC1q
- Reposted by Structura BiotechnologyThis one is a bit of a departure from the usual and definitely a work in progress! We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below. Read on for details... 1/x
- Advanced #cryoEM workflows are easy in #CryoSPARC: use the Low-Level Results Interface to combine upstream extracted particles with downstream pose and CTF information, use a volume result from an intermediate iteration of a refinement to create a mask, and more! guide.cryosparc.com/application-...
- Reposted by Structura BiotechnologyHappy to share a new preprint from the Clarke & Vallese (@fravallese.bsky.social) labs reporting #cryoem structures of two RBC stomatin complexes – with AQP1 & UT-B - continuing the SPFH theme from our recent vault preprint! This was a fun one and has been cooking for a while - read on for more! ⬇️
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- Reposted by Structura BiotechnologyExciting work from the Franklin, @ox.ac.uk, and @diamondlightsource.bsky.social has led to a new method for imaging small proteins (<50 kDa) using cryoEM. By using bifunctional, bispecific nanobody scaffolds, the team have successfully solved the smallest protein structure to date (14 kDa).
- Reposted by Structura BiotechnologyDi-Gembodies (dimerized nanobodies) for improved and duplexed CryoEM of small and challenging targets! A collaboration of @cmd.ox.ac.uk, STRUBI, @diamondlightsource.bsky.social, @rosfrankinst.bsky.social Out now in Nature Chemical Biology @natchembio.nature.com www.nature.com/articles/s41...
- Reposted by Structura BiotechnologyJust published: Our paper on high-throughput analysis of cyclin docking interactions, led by the group of Norman Davey @icr.ac.uk and with some small-complex-plus-peptide #cryoEM by @natalia-mg.bsky.social from my lab. Congratulations, everyone! www.nature.com/articles/s41...
- Flexibility can be frustrating when processing a #cryoEM dataset. If your target has a wobbly domain, check out our new case study on FaNaC1 for continuous heterogeneity tips in #CryoSPARC! guide.cryosparc.com/processing-d...
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- Reposted by Structura BiotechnologyThat's a wrap! The results of the first #cryoEM heterogeneity challenge are up on biorxiv! biorxiv.org/content/10.110
- When particles have orientation bias, the resulting maps are often streaked along the missing directions. In this case study, we cover why orientation bias produces anisotropic #cryoEM maps and how you may be able to recover from it! guide.cryosparc.com/processing-d...
- Link here! guide.cryosparc.com/processing-d...
- Working with symmetric or pseudosymmetric particles in #cryoEM? This TRPV5 and CaM case study includes practical considerations for using symmetry relaxation and 3D classification when analyzing a pseudosymmetric particle in #CryoSPARC!
- Working with symmetric or pseudosymmetric particles in #cryoEM? This TRPV5 and CaM case study includes practical considerations for using symmetry relaxation and 3D classification when analyzing a pseudosymmetric particle in #CryoSPARC!
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- Looking for a deep dive into 2D classification in #cryoEM? These recent guide pages cover practical aspects & parameter choices when running these jobs in #CryoSPARC, along with the theory behind expectation maximization: guide.cryosparc.com/processing-d... and guide.cryosparc.com/expectation-...
- Reposted by Structura Biotechnology1/x New story - the structure of the Vault cap, at 2.3Å! Matching a beautiful preprint from @sjorsscheres.bsky.social, we show that the cap of the vault particle has C13 symmetry, in contrast to the 39-fold symmetry of the body. Study lead by talented postdoc @huanli00.bsky.social from my group! ⬇️
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- Reposted by Structura BiotechnologyExcited to share our new @science.org paper! Led by postdocs Ruchao Peng and Xin Xu, we used cryo-EM/ET to reveal the influenza ribonucleoprotein complex structure and its strand-sliding mechanism for RNA synthesis, paving the way for new antivirals. www.science.org/doi/10.1126/...
- Grappling with poorly-resolved ligand binding sites in #cryoEM maps? Check out our new case study on a peptide ligand-bound GPCR showing how practical tips and features in #CryoSPARC v4.7 improve locally refined map quality and reveal clearer ligand density! guide.cryosparc.com/processing-d...
- Are you new to #CryoEM, or looking for a review of the basics? You might enjoy our TRPV1 case study! It covers preprocessing, particle curation, refinement, and more! You can also test your skills with the advanced exercises at the end of the study. guide.cryosparc.com/processing-d... #CryoSPARC
- #CryoSPARC v4.7 is out! 🚀 Featuring a new automatic Micrograph Junk Detector that labels and rejects contaminants, new tools for curating subsets of particles, performance and stability fixes, and more! Full changelog: cryosparc.com/updates #cryoEM
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- Reposted by Structura BiotechnologyI’m delighted that we could contribute the #cryoEM data to this incredible antiviral discovery project from the group of @neytsjohan.bsky.social 💊 "A coronavirus assembly inhibitor that targets the viral membrane protein" www.nature.com/articles/s41... A huge congrats to everyone involved! 🎉
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- Reposted by Structura Biotechnology*Deadline extended* Applications for this year's #CryoEM Data Processing Workshop are due March 27! Don't miss this chance to learn about various approaches to deal with conformational/compositional heterogeneity. myumi.ch/lsi-cryo-2025 @jbquerido.bsky.social @shyamalm.bsky.social
- Reposted by Structura BiotechnologyWe are thrilled to announce the 2025 EMBO practical course in cryo-em image processing, Birkbeck College, London, 9-16 Sept 2025. More info & apply: meetings.embo.org/event/25-cry... Organisers Giulia Zanetti & Helen Saibil. Beautiful image from co-organizer @carolynmoores1.bsky.social lab.
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