SinghLab@SNIoE
Regenerative & Stress Biology Lab, Shiv Nadar University, Delhi-NCR, India
Regenerative Plasticity | Zebrafish | In vivo Fluorescent Imaging | Liver Regeneration | Starvation Response | Alcoholic Liver Disease
- As part of the Faculty Development Program at Shiv Nadar University , received these 10 commandments for faculty. This is the best advice I have seen, and certainly hits the nail on the head. Especially about focusing and getting the first study out early on.
- 🚨 Just out in Nature Communications: Cholangiocytes contribute to hepatocyte regeneration after partial #liver #injury during growth in #zebrafish We challenge old assumptions on liver regeneration! 🔗 nature.com/articles/s41... 🧵(1/8)
- The liver can regenerate itself—a fact known since the myth of Prometheus. But when hepatocytes are damaged, can cholangiocytes step in to rebuild the liver even if some hepatocytes remain? We built a new zebrafish model to test it. (2/8)
- Enter CellCousin: a new zebrafish tool we designed to trace liver regeneration. 🟢 Spared hepatocytes 🔴 Ablated hepatocytes 🔵 De novo hepatocytes (not hepatocytes before injury) This lets us precisely map where new hepatocytes come from after injury. (3/8)
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View full threadIf you’re interested in liver regeneration, cholangiocyte biology, or cell fate plasticity, we’d love to hear your thoughts! #LiverRegeneration #Zebrafish #RegenerativeBiology #CellPlasticity (8/8)
- Reposted by SinghLab@SNIoE🫀Job alert 🧬 come and be our colleague! Join a BBSRC-funded consortium aiming to understand how the human heart develops - you will be contributing multimodal single cell & spatial transcriptomics data using zebrafish and human cardiac organoids as models. Learn more: www.heartdevelopment.org (1/4)
- Pleased to have contributed commentary to this recent article by Maria Bolevich for Interesting Engineering, which explores the science of tissue #regeneration and its potential applications in medicine. Read the full article here: interestingengineering.com/health/body-...
- We just posted our new preprint, spearheaded by Garnik Hovhannisyan in collaboration with Esteban Gurzov Lab! biorxiv.org/content/10.1... #zebrafish #liverregeneration #DevBio #LineageTracing
- What is CellCousin? It uses stochastic recombination and NTR-based ablation to remove only a subset of hepatocytes. 🟢 Spared cells 🔵 New (De novo) hepatocytes This allows direct comparison of lineage contributions during liver repair.
- What’s new in CellCousin2? ✅ DDCreER: chemically inducible Cre with low leakiness ✅ NTR2.0: ablation with 10× less metronidazole ✅ Cleaner, more precise regeneration readouts The system is modular and adaptable to other tissues or models.
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View full threadHuge thanks to the team and funding support FNRS, DBTIndia that made this happen 🙌 We hope CellCousin2 helps others studying regeneration, cell fate, and tissue repair. All plasmids are available on @addgene.bsky.social
- Stunning 3D fluorescent images of the human #tooth and the first cellular/molecular map of tooth #decay! Hoang Thai Ha from Nicolas Baeyens Lab nails it with tissue clearing + imaging. www.biorxiv.org/content/10.1...
- Reposted by SinghLab@SNIoEInterested in understanding how neural-pancreas interplay contributes to blood sugar control? We have an exciting PhD project to study galanin regulation of pancreatic #islet hormone secretion in live #zebrafish www.exeter.ac.uk/research/ins...
- Be part of the great PhD community at the Living Systems Institute, University Exeter @exeter.ac.uk. Applications for LSI-funded PhD studentships are open until Monday, 9th June 2025. Apply now! www.exeter.ac.uk/research/ins...
- Liver regeneration after killing part of the liver! In this image, the same liver from same #zebrafish is imaged before (left) and after (right) regeneration. The newly born liver cells are in blue. Project led by @selifeski.bsky.social
- We celebrated a big milestone in our lab: our first #Undergrad intern presented her work at lab meeting! She helped develop two new #liver #injury models, and is on her way to becoming a fantastic scientist. Thanks to PhD student Garnik for mentoring her so well! 👏🧪 #TeamScience
- Whole mount EdU staining of 5 day old #zebrafish.
- I'm excited to share that I will join School of Natural Sciences, Shiv Nadar University as an Associate Professor! Looking forward to working with amazing students and being part of a thriving research community.
- Reposted by SinghLab@SNIoECome help us build an inclusive community, for those finterested! Did they mention it’s FREE?!? 🧪
- Delighted to receive FOXO Transcription Factors edited by Dr. Wolfgang Link! FOXO genes are key in stress response & metabolism. Inés Garteizgogeascoa Suñer details a knock-in #zebrafish foxo1a reporter: pubmed.ncbi.nlm.nih.gov/39565585/ Check out the book for key advances!
- Had a great time meeting the students at Freedom Employability Academy (FEA: feaindia.org ) today! Inspiring to see their enthusiasm and growth. Excited to support their journey towards meaningful careers! #Mentorship #Education
- Reposted by SinghLab@SNIoEWe demonstrate that this biphasic regulation limits the neutrophil-driven acute inflammatory phase, preventing chronic inflammation, fostering a lesion environment with mechano-structural properties conducive to regeneration.
- Reposted by SinghLab@SNIoEExcited to share the latest work from our lab: www.biorxiv.org/content/10.1...
- Reposted by SinghLab@SNIoEFantastic collaboration with the labs of Sven Falk, @singhlab.bsky.social, Jochen Guck, @franzelab.bsky.social, Kanwarpal Singh, and Federico Marini. @fau.de @mpi-scienceoflight.bsky.social Johannes Gutenberg University University of Cambridge @iribhm.bsky.social, @mcmasteruniversity.bsky.social
- Reposted by SinghLab@SNIoEWe are openly recruiting at all levels at the Tornini Lab at the University of California, Los Angeles (UCLA)! Please share with your networks. For more information, please check the info below, visit www.torninilab.org, or email me directly at vtornini at ucla dot edu. Thank you in advance!
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- Reposted by SinghLab@SNIoEFancy joining our lab? apply here for a PhD student position, (DM me for potential postdoc opportunities) www.uu.se/en/about-uu/...
- Reposted by SinghLab@SNIoECheck out our preprint about liver regeneration, showing hepatic duct heterogeneity and cell plasticity using lineage tracing and single-cell transcriptomics. @zebrafishrock.bsky.social @biorxivpreprint.bsky.social @the-node.bsky.social www.biorxiv.org/content/10.1...
- Excited to share our latest work on #liver #regeneration in @biorxiv-devbio.bsky.social ! We uncover how cholangiocytes contribute to hepatocyte regeneration, even in the presence of spared hepatocytes, using our novel CellCousin model in #zebrafish: www.biorxiv.org/content/10.1... 🐟✨ A 🧵👇 (1/12)
- The liver’s regenerative capacity is legendary—think Prometheus. 🔥 But what happens when hepatocytes can't do the heavy lifting? Enter cholangiocytes, which can transdifferentiate into hepatocytes under certain conditions. But how? Why? And when? (2/12)
- Using #zebrafish, a powerful model for studying liver biology due to its transparency and genetic tractability, we developed CellCousin—a system for lineage tracing and quantifying hepatocyte regeneration after injury. (3/12)
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View full threadAnd if you’re interested in regenerative biology, visit our lab's website: sumeetpalsingh.github.io 🚀💡(12/12) #LiverRegeneration #Zebrafish
- Reposted by SinghLab@SNIoE5,562 proteins were found to have tissue-specific enrichment across 32 human tissues. The majority of them (3,088) are enriched at the protein but not RNA level. The brain hosts the largest number of proteins that are only enriched at the protein level but not RNA level.
- What shapes the #immune profile of organs? Study from Ninov Lab #CRTD, led by Nadeem, find that developmental #celldeath define the immune cells of the pancreatic #islet in #zebrafish ! 🎉👏 Read more @embojournal.bsky.social : embopress.org/doi/full/10.... #Diabetes
