JungmannLab
Our group at LMU Munich and @mpibiochem.bsky.social uses DNA nanotechnology to develop next-generation super-resolution microscopy techniques. #DNAPAINT
- Reposted by JungmannLabHow do you scale super-resolution microscopy to dozens of proteins without linearly scaling imaging time? We introduce Combi-PAINT: a combinatorial DNA-PAINT strategy that breaks the 1-target-per-round bottleneck of Exchange-PAINT: www.biorxiv.org/content/10.6... 1/5
- Reposted by JungmannLabRalf Jungmann, of the @mpibiochem.bsky.social, is the first speaker to step to the BiOS Hot Topics stage at #PhotonicsWest!💡 He is delivering his presentation titled: “From DNA nanotechnology to biomedical insight: towards single-molecule spatial omics” @jungmannlab.bsky.social
- Reposted by JungmannLabNow online: October 2025 Issue. - Focus Issue on #biosensing, - DNA moiré superlattices, - Sugars at Ångström-resolution, - Solid-state #nanopores, - Non-aqueous Li #batteries, - - Neuromorphic vision, - Peptide #hydrogels, - Deep learning for #LNPs and more... www.nature.com/nnano/volume...
- Highly efficient 12-color multiplexing with speed-optimized DNA-PAINT. We are excited to share our latest paper in @natcomms.nature.com, using left-handed DNA to extend speed-optimized DNA-PAINT to 12 targets in a simple and straightforward way! 🧬👈🚀https://www.nature.com/articles/s41467-025-64228-x
- The mirrored design of left-handed oligonucleotides allows the extension of the common 6 speed-sequences R1-R6 with their analogs L1-L6, enabling 12 target multiplexing with a standard secondary label-free DNA-PAINT workflow. (2/6)
- We demonstrate speed-optimized left-handed DNA-PAINT by characterizing the sequence binding kinetics and resolving three main microscopy benchmarking targets, mitochondria, microtubules and nuclear pore complexes with <5 nm localization precision. (3/6)
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View full threadThanks to all who made this possible! @eduardunterauer.bsky.social @evaschentarra.bsky.social @ipachmayr.bsky.social @taishatashrin.bsky.social Jisoo Kwon Sebastian Strauss, @jekristina.bsky.social @rafalkowalew.bsky.social @opazo.bsky.social @forna.bsky.social @lumasullo.bsky.social (6/6)
- Reposted by JungmannLabNext on stage is Eduard Unterauer @eduardunterauer.bsky.social from @jungmannlab.bsky.social reporting spatial proteomics with DNA PAINT #SMLMS2025
- We're excited that the study is now out in Nature Nanotechnology @natnano.nature.com www.nature.com/articles/s41...
- ÅNGSTRÖM-RESOLUTION IMAGING OF CELL-SURFACE GLYCANS 🧬🎨🍬 The glycocalyx, our cells' sugar coat, holds secrets in immunology, cancer, viral infections, and more. Visualizing its molecular architecture was impossible… until now. #glycotime #microscopy www.biorxiv.org/content/10.1...
- Reposted by JungmannLabNew paper online: Ångström-resolution imaging of cell-surface glycans. The molecular organization of sugars in the native #glycocalyx has been resolved at 9 ångström using bioorthogonal metabolic labeling and #superresolution imaging of DNA barcodes. #Glycotime www.nature.com/articles/s41...
- Ever wondered what happens when therapeutic antibodies bind to cancer cells? In our latest study, we used multiplexed 3D-RESI to directly visualize how anti-CD20 antibodies interact with their receptors, revealing their precise arrangement at single-protein resolution. (1/6)
- By imaging intact cells, we could see these therapeutic antibodies in action: Type I antibodies like Rituximab and Ofatumumab formed extended chains of CD20 hexamers or larger, creating platforms compatible with complement protein binding, mediating cancer cell killing. (2/6)
- In contrast, Type II antibodies like Obinutuzumab and H299 induced limited oligomerization to dimers, trimers and tetramers, consistent with their role in promoting direct tumor cell death rather than complement activation. (3/6)
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View full threadCongratulations to everyone involved: @ipachmayr.bsky.social, @lumasullo.bsky.social, @susannereinhardt.bsky.social, Jisoo Kwon, Ondřej Skořepa, Maite Llop, Sylvia Herter, Marina Bacac and Christian Klein. (6/6) Read the full story here: www.nature.com/articles/s41...
- Reposted by JungmannLabCongratulations to Ralf on your election as a new EMBO member: ❕Original press release from @embo.org : www.embo.org/press-releas...
- Great science, great company and stunning views at our Lab retreat on Schloss Ringberg 🏰🧬🔬. Big thanks to our guests Sabrina Simoncelli, Sebastian Kobold, Thomas Schlichthärle, @massivephotonics.bsky.social & students from the @lfmilles.bsky.social and @mlsb-borgwardt.bsky.social Labs for joining!
- Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution We are excited to present our latest work published in @natcomms.nature.com www.nature.com/articles/s41...
- Understanding how proteins assemble into complexes (oligomerize) within their native cellular environments is crucial for deciphering cellular signaling pathways 1/7
- Latest advances in super-resolution microscopy (DNA-PAINT, MINFLUX, RESI) allow the study of molecular arrangements at the level of single proteins, but extracting quantitative information on the 1–20 nm scale through rigorous image analysis remains a significant challenge. 2/7
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