Yulong Li Lab
Yulong Li lab at Peking University
- (1/3)Excited to share PKU tags—our lab’s latest contribution expanding the toolkit for cell-type labeling with a new shape dimension! Developed at PKU, these genetically encoded tools enable multiplex cell-type labeling compatible with both light and electron microscopy.
- (2/3)By integrating self-assembling protein polymers, spectrally distinct fluorescent proteins, and a nuclear targeting motif, PKU tags provide a versatile palette of cell-labeling tools. With combinatorial strategy, the system can theoretically scale to hundreds of patterns.
- (3/3)Moreover, we showcased the power of PKU tags for in vivo shape-based “barcoding’’ of multiple cell types, delivering high-contrast and easily distinguishable signals in both light and electron microscopy.
- BioRxiv link www.biorxiv.org/cgi/content/...
- We’re bringing our latest progress in neuromodulator imaging to #SfN2025! Don’t miss Dr. Li’s talk (Nov 19) and our lab’s posters (Nov 16). We look forward to connecting, exchanging ideas, and exploring collaborations. #neuroscience #neuroimaging #biosensors @sfn.org
- Glad to see Yu Zheng PhD @zheng-yu.bsky.social highlighting the far-red dopamine sensor for multiplex imaging of in vivo neuromodulation. Happy to share these probes to the community.
- A far-red dopamine sensor unlocks multiplex views of in vivo neuromodulation — a Tools of the Trade article by Yu Zheng #neuroscience #neuroskyence www.nature.com/articles/s41...
- Having trouble getting the probes or dyes? Contact us at yulonglilab2018@gmail.com — we will be more than happy to help.
- 1/ Excited to share a new preprint! Our latest study uncovers how serotonin precisely controls the “time window” for fear learning, ensuring that our brains link cues (CS) & threats (US) only when it’s adaptive. #Neuroscience #FearLearning www.biorxiv.org/content/10.1...
- 2/ The brain needs tight temporal boundaries for associations of cues and threats. Too narrow → We might miss real threats. Too wide → We might create false alarms. The question: What keeps this window just right?
- 3/ We found that serotonin is the key. In trace fear conditioning, systemic serotonin manipulations bidirectionally shift the associable interval. Using our 5-HT3.0 sensor, we showed real-time, in vivo evidence that serotonin release patterns in vCA1 tightly track this time window.
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View full thread6/ Sustained serotonin release in vCA1 keeps 5-HT2C–expressing pyramidal neuron ensembles, linking temporally separated events. When the gap exceeds the brain’s “safe window”, brief serotonin release fails to drive these ensembles, thus preventing maladaptive learning.
- (1/3) Excited to introduce our new GRAB sensors for a series of steroid hormones! These tools enable real-time detection of steroid hormone dynamics in vivo🐭🧠. Happy to share these sensors and welcome any feedback! Please contact yulonglilab2018@gmail.com for information.
- (2/3) Applying Prog1.0 in vivo: We detected both the maternal behavior-associated🤱 and spontaneous PROG signals ☀️🌙in the hypothalamus with Prog1.0 by fiber photometry.
- (3/3) Applying Cort1.0 in vivo: We observed stress-induced 😰CORT elevation in the hypothalamus (e.g., during tail suspension) with Cort1.0 by fiber photometry. Real-time stress hormone readouts, live!
- Excited to share our Science paper on HaloDA1.0-the first genetically encoded far-red dopamine sensor! 🥳It enables powerful multiplex imaging in neurons, brain slices, and live animals.🧠🔬 #GRABsensors #Dopamine www.science.org/doi/10.1126/...
- Amazing work by Yu Zheng @zheng-yu.bsky.social， Ruyi Cai @ruyic.bsky.social, and the whole team. Huge thanks to our fantastic collaborators: Yu Mu, Zhixing Chen (@zhixingchen2.bsky.social), Luke Lavis,Eric Schreiter, Kai Johnsson, and Jonathan Grimm.
- Reposted by Yulong Li LabDeepest thanks to @yulonglilab.bsky.social and all those who attended his seminar on GRAB sensors in neuromodulator dynamics hosted by @maximoprado.bsky.social at @westernu.bsky.social.
