Have you been interested combining Perturb-Seq screening with Cas12a’s unique capabilities for multiplexed guide RNA expression? Us too! So, we figured out some useful tricks to make that possible, which you can find in our new pre-print. Short thread below:
www.biorxiv.org/content/10.1...
Degron-modified Cas12a enhances single-cell CRISPR screening
Single-cell CRISPR (Perturb-seq) screens have primarily relied on Cas9 whereas Cas12a, despite its unique effectiveness for multiplex guide expression, remains underexplored. This may be due to Cas12a...
