- Our latest by the very talented postdoc Dr. Samridhi Pathak, “Differential gene expression drives cell-cycle-dependent transition from monopolar to bipolar growth in fission yeast.” Now available on @biorxiv-cellbio.bsky.social 1/x
- Fission yeast is a great model for cell polarity research. These cells start out monopolar and on reaching a certain size become bipolar. Previously we showed that the cell ends compete with each other and bipolarity ensues when cells grow, make more material and overcome the competition. 2/x
- However, G1/S-arrested cells fail to become bipolar even at a larger cell size. This suggests that bipolarity is cell-cycle dependent. The basic polarity factors including Cdc42 and its regulators do not seem to behave differently in G1/S suggesting that the regulation may be at a systemic level.3/xAug 16, 2025 19:47
- To identify the factors promoting the transition from monopolar to bipolar growth, we analyzed differential gene expression patterns between G1/S and G2 phase cells. We used the cdc10-129 mutants that arrest in G1/S under restrictive conditions. 4/x
- Using a combination of GSEA and KEGG analysis, protein-protein interaction networks and validation with cell biology we identified that the nutritional stress response pathway that promotes G1-arrest is upregulated in G1/S phase and prevents precocious bipolar growth. 5/x
- G2 phase cells upregulate cellular component biogenesis, cell-wall organization, and plasma membrane maintenance pathways to facilitate bipolar growth. 6/x
- Our results suggest that the stress response pathway needs to be downregulated not only for cell-cycle progression but also for the transition to bipolarity. These findings highlight the interplay between cell cycle, growth, stress response and polarity pathways.7/end www.biorxiv.org/content/10.1...
