Amir Rahmani
Super-resolution microscopy enthusiast
Postdoc at the University of Cambridge
Visiting Researcher at the British Antarctic Survey
College Research Associate at Wolfson College
laser.ceb.cam.ac.uk
- Haven’t taken a flight in 14 months ✈️ Won’t take one until April either, which’ll make it 17. Just a reminder that travel choices do add up for CO₂. One person skipping a flight doesn’t ground a plane, but lower demand over time does mean fewer routes and frequencies.
- Crafting New Year (super-)resolutions at cold for 2026 Happy New Year everyone 🎉
- Reposted by Amir RahmaniMerry Christmas to all our students, staff, alumni and friends around the world 🎄 📸 Lloyd Mann
- Reposted by Amir RahmaniBored over the holidays? Give our new preprint on how #microtubule lattice alteration by taxols can regulate #RhoA #signalling via GEF-H1 a read! Perhaps a new mechanism of action for taxol #chemotherapeutics during interphase. www.biorxiv.org/content/10.6... Happy holidays ✨🎄
- Reposted by Amir RahmaniLast project of the year: self blinking JF635 + lattice light sheet + #BigVolumeBrowser to deskew both volume and localization data (and render) @zeiss-microscopy.bsky.social
- ✨ Blinking #nanobodies that work for single-molecule localization 🔬 Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!
- Nice collection of cameras!
- Reposted by Amir Rahmani✨ Blinking #nanobodies that work for single-molecule localization 🔬 Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!
- I was incredibly fortunate in this! The ups and downs were many but all those discussions helped make sense of a few scientific questions that are still ongoing and lead to further questions.
- Reposted by Amir RahmaniTracking coordinated cellular dynamics directly from images -- New method paper by labmates @bgraedel.bsky.social & @macdobry.bsky.social ! Python package & @napari.org plugin, all the good stuff: Paper: doi.org/10.1242/jcs.... Code: github.com/pertzlab/arc... Plugin: github.com/pertzlab/arc...
- Reposted by Amir RahmaniNew paper out! Combining single-objective light-sheet microscopy and time-resolved SPAD array detection, we massively accelerate fluorescence lifetime imaging (FLIM) compared to confocal FLIM, making FLIM applicable to 3D specimen such as organoids and embryos. www.nature.com/articles/s42...
- Reposted by Amir RahmaniMy shirt is cooler than yours
- Fantastic night at Christ’s college for the LAG-MNG Christmas meal! @lasergroup.bsky.social
- I won’t buy a lens from you after seeing you put it upside down. 🙃
- Nature’s optics at its best! A full lunar halo lighting up the night.
- Reposted by Amir RahmaniAnother huge advantage of miOPM is that it's gentle! A light-sheet microscope that's compatible with standard slides and multi-well plates! -> Here we show 3D mitochondrial membrane potential in mammalian cells (GFP/RFP for mito, Cy5 for DNA). The cells move around freely! doi.org/10.1101/2025...
- Reposted by Amir RahmaniPhD studentship available on using our single-molecule flow cytometry technology to detect rare proteins. Apply if you're interested in applying single-molecule techniques to improve diagnostics and to discover new routes for anti-cancer immunotherapy. www.findaphd.com/phds/project...
- Always there for our group meetings!
- Reposted by Amir RahmaniSingle-molecule friends! The prelim. program of next year's sm- #Gordon conference in Switzerland is online: www.grc.org/single-molec... It'll be 3x fantastic: science, people, place in the alps ⛰️. Kevin & I hope to see you all next June: Have a look, tell your friends, and register!
- Reposted by Amir Rahmani
- Thrilled to see interest in this preprint! This work was a major part of my PhD, my hope is that single-molecule flow cytometry can be useful to the community in exploring new applications and discoveries. doi.org/10.1101/2025...
- 👀 What if your flow cytometer could see the invisible? Our new preprint introduces single-molecule flow cytometry (smFC), pushing detection sensitivity 10–80× beyond conventional flow cytometry. www.biorxiv.org/content/10.1...
- 👀 What if your flow cytometer could see the invisible? Our new preprint introduces single-molecule flow cytometry (smFC), pushing detection sensitivity 10–80× beyond conventional flow cytometry. www.biorxiv.org/content/10.1...
- Conventional flow cytometry is powerful but has a blind spot: ❌ It can’t detect proteins expressed at very low levels (<100–1000 molecules/cell). That means many weak but biologically crucial signals are invisible.
- smFC combines: 🔬 High-NA oblique plane microscopy (OPM) 💧 Microfluidics ✨ Superbright, large Stokes shift dyes → enabling optical sectioning + photon efficiency needed for single-molecule detection in flowing cells.
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View full threadHuge thanks to an incredible team who made this possible 🙌 especially Aleks for his great supervision and support.
- Reposted by Amir RahmaniHow many photons are in a GFP? — more than last year, and more than you thought. Here's a simple, cheap, and practical method to break a fundamental limit in fluorescence microscopy. But it only works in light sheet!
- Reposted by Amir RahmaniI was searching for a Python utility for Fourier Ring/Shell Correlation. All I got in a blue sky search were 4 small posts from @christletx.bsky.social ... all I got from chatgpt was installation instructions for non-existent libraries and github repos... anyone know a good tool for this?
- Reposted by Amir RahmaniThe review on live-cell SMT that I contribute to the jmolbiol.bsky.social special issue ‚Imaging of the central dogma‘ is now online as pre-proof: www.sciencedirect.com/science/arti...
- Reposted by Amir RahmaniProduction has started for a new #Snoutscope objective, AMS-AGY v3. It has significantly larger FOV and some other tweaks from v1 and v2. Currently accepting pre-orders. The price will need to increase modestly after deliveries start late October.
- Reposted by Amir Rahmani📽️ G&D Tapes 📽️ G&D author, Noah Helton tells us about their new study in #genesdev, revealing an intriguing link between stress granule formation and the integrated stress response. #OpenAccess @smslmoon.bsky.social Read the full story here: ➡️ tinyurl.com/gd352899
- Excellent choice of photo to begin the talk! Speaker: Dr Meng Lu from Peking University
- Reposted by Amir RahmaniCheck out our latest preprint, which is the latest production from our long-lasting collaboration with Andrey Klymchenko > functionnalizing NPs with humanized antibodies (i.e. Trastuzumab). Work performed by @vmittelheisser.bsky.social & O.Lefebvre in my lab. www.biorxiv.org/content/10.1...
- Reposted by Amir Rahmani[This post could not be retrieved]
- They showed how the conformational dynamics of single molecules can be retrieved from FRET correlation functions in diffusion-based smFRET experiments. www.nature.com/articles/s41...
- Slanted light-sheet array microscopy for large volume imaging at rates exceeding 100 Hz arxiv.org/pdf/2506.13664
- A review on cytoplasm to explore how cytoplasmic organisation contributes to metabolic regulation! pubs.acs.org/doi/full/10....
