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- Since all Illumina sequencing involves some PCR (bridge amplification), I also wonder at what length Illumina reads start to fail with homopolymers. Can they reliably sequence 20-mers? 40-mers? 60-mers? It's a hard question to answer if every sequencing tech struggles with these...
- Definitely hard to measure, but here is our best attempt from a new benchmarking paper to be preprinted in the next few weeks. Element appears to be the current king of homopolymers. Illumina does worse than HiFi beyond 20 bp. (This is all measured on human DNA)Sep 5, 2025 12:55
- It's wild how different the illumina error % is between the two plots, at 15 nt homopolymer one shows 2% error and the other 20% error (phred 7 vs ca 17). Or am I missing something? bsky.app/profile/albe...
