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Nature Protocols

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Nature Protocols natprot.nature.com · 23h
New Article! Simplified ischemia-free liver transplantation with continuous normothermic machine perfusion
Simplified ischemia-free liver transplantation with continuous normothermic machine perfusion

Nature Protocols, Published online: 06 February 2026; doi:10.1038/s41596-025-01321-xThis Protocol describes a simplified ischemia-free liver transplant procedure, where the portal vein is directly cannulated into the donor portal vein, eliminating the need for an interposition vein.

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Nature Protocols natprot.nature.com · Feb 4
New Article! Designing and testing CRISPRi-based synthetic gene circuits in plants
Designing and testing CRISPRi-based synthetic gene circuits in plants

Nature Protocols, Published online: 04 February 2026; doi:10.1038/s41596-025-01312-yThis protocol details the design principles and procedures for building programmable CRISPRi-based gene circuits, and for testing individual components and the assembled circuits in various plant species using a high-throughput protoplast-based assay.

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Nature Protocols natprot.nature.com · Feb 4
New Article! Conjugation of hydrophobic drugs to motile pRNA 4WJ nanoparticles for spontaneous tumor targeting and undetectable toxicity
Conjugation of hydrophobic drugs to motile pRNA 4WJ nanoparticles for spontaneous tumor targeting and undetectable toxicity

Nature Protocols, Published online: 03 February 2026; doi:10.1038/s41596-025-01306-wThis protocol describes the synthesis, purification and assembly of stable branched 4-way junction (4WJ) RNA nanoparticles conjugated with multiple hydrophobic chemotherapeutic drug molecules to target specific tumors with minimal side effects.

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Nature Protocols natprot.nature.com · Feb 3
New Article! Secreted luciferases as a minimally invasive 3R-compliant tool for accurate monitoring of tumor burden
Secreted luciferases as a minimally invasive 3R-compliant tool for accurate monitoring of tumor burden

Nature Protocols, Published online: 03 February 2026; doi:10.1038/s41596-025-01315-9This protocol details in vitro and in vivo approaches for labeling tumor cells with secreted luciferases to enable precise, longitudinal monitoring of tumor growth in mice, through the analysis of small blood samples.

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Nature Protocols natprot.nature.com · Feb 3
New Article! INFOGEST Quant: standardized in vitro determination of digestibility and DIAAS of dietary proteins based on the INFOGEST static digestion model
INFOGEST Quant: standardized in vitro determination of digestibility and DIAAS of dietary proteins based on the INFOGEST static digestion model

Nature Protocols, Published online: 03 February 2026; doi:10.1038/s41596-025-01307-9This Protocol Extension is an adapted version of the authors’ previous INFOGEST static digestion model (INFOGEST 2.0), with an added workflow for the quantification of total protein digestibility, individual amino acid digestibility and digestible indispensable amino acid score.

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Nature Protocols natprot.nature.com · Feb 2
New Article! Magnetoencephalography in human neuroscience research: planning, piloting, implementation and quality assurance
Magnetoencephalography in human neuroscience research: planning, piloting, implementation and quality assurance

Nature Protocols, Published online: 02 February 2026; doi:10.1038/s41596-025-01295-wA protocol for the planning and implementation of magnetoencephalography experiments across nonclinical and clinical settings, including the methodological considerations required for designing multidisciplinary, population-based studies.

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