Chancie T
Senior Scientist @ Nxera Pharma 🇬🇧
Lab alumni: @cpaulino.bsky.social & @membraneenzymology.bsky.social (Slotboom) @ RUG 🇳🇱 & @mitocarriers.bsky.social (Kunji) @ MRC MBU/Cambridge University 🇬🇧
#MembraneProteins, #Transporters, #GPCRs, #cryoEM, #SBDD
- Reposted by Chancie THere, we critically review the structures of the mitochondrial pyruvate carrier (MPC) in apo and ligand-bound states, highlighting controversies with regard to the orientation in the inner membrane, interpretation of the transport states, and modelling of the inhibitors. doi.org/10.1016/j.ti...
- Reposted by Chancie TOur review on the peculiar properties of mitochondrial carriers of the SLC25 family out: portlandpress.com/biochemj/art...
- Check out our new preprint on the bacterial secondary active #transporter SbmA.
- We have captured occluded and inward-facing states by #cryoEM elucidating the conformational changes associated with proton binding and substrate translocation.
- The conformational states resemble those found in primary active transporters of the #ABC superfamily, and our bioinformatic analysis shows that SbmA-like peptide transporters (SLiPTs) likely evolved from an ancestral ABC transporter.
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View full threadPleased to share that the 200 kV reconstructions in this paper have been released today: 1. www.emdataresource.org/EMD-50994 2. www.emdataresource.org/EMD-50995 3. www.emdataresource.org/EMD-50996 4. www.emdataresource.org/EMD-50997 5. www.emdataresource.org/EMD-50998
- Reposted by Chancie TSolène’s 4.5 years of postdoctoral work in our lab (she started just before the corona pandemic) has now been published. A heroic effort that revealed -at the single molecule level- that ATP drives the dissociation of an ECF transporter complex under turnover conditions. See rdcu.be/elTKc
- Reposted by Chancie TA colder frontier in cryo-EM 🧪🔬 Chris Russo’s group, inc. Joshua Dickerson, adapted #cryoEM to work at liquid helium temperatures (13 kelvins), where every frame captured contains more information than the equivalent using liquid nitrogen (81 kelvins). Read more: tinyurl.com/mwwcunkc #LMBResearch
- Reposted by Chancie TOur paper is finally out: Molecular basis of pyruvate transport and inhibition of the human mitochondrial pyruvate carrier | Science Advances www.science.org/doi/10.1126/... #mitochondria #cryo-EM
- Reposted by Chancie TThe work was a collaboration with Nate Traaseth. Hope the method will help others solve structures of small membrane proteins in multiple conformations. The fusion strategy can be seen as a tribute to the late Ron Kaback, who attempted a similar approach with crystallizing the LacY 30 years ago.
- Reposted by Chancie TAt the heart of biology is the mantra: structure dictates function.Biological structures are also beautiful in their intricacy. David Goodsell brought this into public view. As he leaves "Molecule of the Month". here's a huge thank you for helping me see more clearly🧪1/2 cdn.rcsb.org/rcsb-pdb/gen...
- Reposted by Chancie TUsing 33 pathogenic variants of citrin we identify crucial elements of the carrier domain required for transport and show that the N-terminal domain is not involved in calcium regulation of transport, but causes a mitochondrial import defect, when mutated. www.sciencedirect.com/science/arti...
- Reposted by Chancie TOur new paper is out! #mitochondria #transporters #adeninetranslocase #SLC25 www.science.org/doi/10.1126/...
